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AB113462

KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric)

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(2 Publications)

KDM4/JMJD2 (Lysine-specific demethylase 4) is a family of histone demethyltransferases involved in H3(meK9) specific demethylation.

View Alternative Names

JHDM3A, JMJD2, JMJD2A, KIAA0677, KDM4A, Lysine-specific demethylase 4A, JmjC domain-containing histone demethylation protein 3A, Jumonji domain-containing protein 2A, [histone H3]-trimethyl-L-lysine(36) demethylase 4A, [histone H3]-trimethyl-L-lysine(9) demethylase 4A

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Functional Studies - KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (AB113462)
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Supplier Data

Functional Studies - KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (AB113462)

Demonstration of high sensitivity of the KDM4/JMJD2 activity assay achieved by using recombinant KDM4/JMJD2 with ab113462.

Key facts

Detection method

Fluorescent

Assay type

Enzyme activity

Assay time

3h

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

KDM4/JMJD2 (Lysine-specific demethylase 4) is a family of histone demethyltransferases involved in H3(meK9) specific demethylation. The KDM4/JMJD2 family are JmjC proteins that specifically removes tri-methylation from H3(meK9) and H3(meK36) but has no activity on mono- or di-methylated residues.

KDM4/JMJD2 Activity Quantification Assay Kit (Fluorometric) (ab113462) is designed for the easy and fast fluorometric measurement of KDM4/JMJD2 activity or inhibition. The antibody-based, immunospecific method directly detects KDM4/JMJD2-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab113462-96Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Adhesive Covering Film", "number":"AB113462-CMP05" }, { "size":"1 x 20 µL", "name":"Fluoro Developer", "number":"AB113462-CMP09" }, { "size":"1 x 10 µL", "name":"Capture Antibody, 1000 µg/mL", "number":"AB113462-CMP07" }, { "size":"1 x 28 mL", "name":"10X Wash Buffer", "number":"AB113462-CMP06" }, { "size":"1 x 60 µL", "name":"Co-factor 3", "number":"AB113462-CMP04" }, { "size":"1 x 8 mL", "name":"JMJD2 Assay Buffer", "number":"AB113462-CMP12" }, { "size":"1 x 60 µL", "name":"Co-factor 2", "number":"AB113462-CMP03" }, { "size":"12 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB113462-CMP01" }, { "size":"1 x 20 µL", "name":"JMJD2 Assay Standard, 50 µg/mL", "number":"AB113462-CMP13" }, { "size":"1 x 120 µL", "name":"JMJD2 Substrate, 50 µg/mL", "number":"AB113462-CMP14" }, { "size":"1 x 12 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB113462-CMP08" }, { "size":"1 x 20 µL", "name":"Fluoro Enhancer", "number":"AB113462-CMP10" }, { "size":"1 x 8 mL", "name":"Fluoro Dilution", "number":"AB113462-CMP11" }, { "size":"1 x 60 µL", "name":"Co-factor 1", "number":"AB113462-CMP02" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab113462-48Test", "assetComponentDetails": [ { "size":"1 x 30 µL", "name":"Co-factor 1", "number":"AB113462-CMP02", "productcode":"" }, { "size":"1 x 10 µL", "name":"Fluoro Developer", "number":"AB113462-CMP09", "productcode":"" }, { "size":"1 x 5 µL", "name":"Capture Antibody, 1000 µg/mL", "number":"AB113462-CMP07", "productcode":"" }, { "size":"1 x 4 mL", "name":"Fluoro Dilution", "number":"AB113462-CMP11", "productcode":"" }, { "size":"1 x 10 µL", "name":"Fluoro Enhancer", "number":"AB113462-CMP10", "productcode":"" }, { "size":"1 x 30 µL", "name":"Co-factor 2", "number":"AB113462-CMP03", "productcode":"" }, { "size":"1 x 6 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB113462-CMP08", "productcode":"" }, { "size":"1 x 10 µL", "name":"JMJD2 Assay Standard, 50 µg/mL", "number":"AB113462-CMP13", "productcode":"" }, { "size":"6 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB113462-CMP01", "productcode":"" }, { "size":"1 x 60 µL", "name":"JMJD2 Substrate, 50 µg/mL", "number":"AB113462-CMP14", "productcode":"" }, { "size":"1 x 14 mL", "name":"10X Wash Buffer", "number":"AB113462-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Adhesive Covering Film", "number":"AB113462-CMP05", "productcode":"" }, { "size":"1 x 4 mL", "name":"JMJD2 Assay Buffer", "number":"AB113462-CMP12", "productcode":"" }, { "size":"1 x 30 µL", "name":"Co-factor 3", "number":"AB113462-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code (PubMed : 26741168). Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.. Isoform 2. Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain.
See full target information KDM4A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:641 PubMed35869047

2022

Accumulation of oncometabolite D-2-Hydroxyglutarate by SLC25A1 inhibition: A metabolic strategy for induction of HR-ness and radiosensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Kexu Xiang,Christian Kalthoff,Corinna Münch,Verena Jendrossek,Johann Matschke

Proceedings of the National Academy of Sciences of 111:9235-40 PubMed24928520

2014

Chem-seq permits identification of genomic targets of drugs against androgen receptor regulation selected by functional phenotypic screens.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyu Jin,Liuqing Yang,Min Xie,Chunru Lin,Daria Merkurjev,Joy C Yang,Bogdan Tanasa,Soohwan Oh,Jie Zhang,Kenneth A Ohgi,Hongyan Zhou,Wenbo Li,Christopher P Evans,Sheng Ding,Michael G Rosenfeld
View all publications
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