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KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation.

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Detection method

Colorimetric

Assay type

Enzyme activity

Reactive species

Mouse, Human

Assay time

3h

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Images

Target data

What's included?

96 Test
Components
10X Wash Buffer
1 x 28 mL
8-Well Assay Strips (with Frame)
12 x 1 Unit
Adhesive Covering Film
1 x 1 Unit
Capture Antibody, 1000 µg/mL
1 x 10 µL
Co-factor 1
1 x 60 µL
Co-factor 2
1 x 60 µL
Co-factor 3
1 x 60 µL
Detection Antibody, 400 µg/mL
1 x 12 µL
Developer Solution
1 x 10 mL
JARID Assay Buffer
1 x 8 mL
JARID Assay Standard, 50 µg/mL
1 x 20 µL
JARID Substrate, 50 µg/mL
1 x 120 µL
Stop Solution
1 x 10 mL

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KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Colorimetric

Assay type

Enzyme activity

Reactive species

Mouse, Human

Assay time

3h

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation. KDM5/JARID demethylases are JmjC proteins that remove di- and tri-methylation from H3(meK4) but have no activity on mono-methylated residues.

KDM5/JARID Activity Quantification Assay Kit (Colorimetric) (ab113463) is designed for the easy and fast colorimetric measurement of KDM5/JARID activity or inhibition. The antibody-based, immunospecific method directly detects KDM5/JARID-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

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Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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