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KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation.

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Images

Functional Studies - KDM5/JARID Activity Quantification Assay Kit (Colorimetric) (AB113463), expandable thumbnail

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Key facts

Detection method
Colorimetric
Assay type
Enzyme activity
Reactive species
Mouse, Human
Assay time
3h

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Target data

Function

Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif (PubMed:18270511). May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation (PubMed:19430464). May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1 (By similarity). Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells (PubMed:27427228).

Alternative names

JARID1A, RBBP2, RBP2, KDM5A, Lysine-specific demethylase 5A, Histone demethylase JARID1A, Jumonji/ARID domain-containing protein 1A, Retinoblastoma-binding protein 2, [histone H3]-trimethyl-L-lysine(4) demethylase 5A, RBBP-2

What's included?

96 Test
Components
10X Wash Buffer
1 x 28 mL
8-Well Assay Strips (with Frame)
12 x 1 Unit
Adhesive Covering Film
1 x 1 Unit
Capture Antibody, 1000 µg/mL
1 x 10 µL
Co-factor 1
1 x 60 µL
Co-factor 2
1 x 60 µL
Co-factor 3
1 x 60 µL
Detection Antibody, 400 µg/mL
1 x 12 µL
Developer Solution
1 x 10 mL
JARID Assay Buffer
1 x 8 mL
JARID Assay Standard, 50 µg/mL
1 x 20 µL
JARID Substrate, 50 µg/mL
1 x 120 µL
Stop Solution
1 x 10 mL

Recommended products

KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation.

Key facts

Detection method
Colorimetric
Assay type
Enzyme activity
Reactive species
Mouse, Human
Assay time
3h
Assay Platform
Microplate reader

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation. KDM5/JARID demethylases are JmjC proteins that remove di- and tri-methylation from H3(meK4) but have no activity on mono-methylated residues.

KDM5/JARID Activity Quantification Assay Kit (Colorimetric) (ab113463) is designed for the easy and fast colorimetric measurement of KDM5/JARID activity or inhibition. The antibody-based, immunospecific method directly detects KDM5/JARID-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

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1 product image

  • Functional Studies - KDM5/JARID Activity Quantification Assay Kit (Colorimetric) (ab113463), expandable thumbnail

    Functional Studies - KDM5/JARID Activity Quantification Assay Kit (Colorimetric) (ab113463)

    Demonstration of high sensitivity of the KDM5/JARID activity assay achieved by using A549 nuclear extracts with ab113463.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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