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AB113464

KDM5/JARID Activity Quantification Assay Kit (Fluorometric)

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KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation.

View Alternative Names

JARID1A, RBBP2, RBP2, KDM5A, Lysine-specific demethylase 5A, Histone demethylase JARID1A, Jumonji/ARID domain-containing protein 1A, Retinoblastoma-binding protein 2, [histone H3]-trimethyl-L-lysine(4) demethylase 5A, RBBP-2

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Functional Studies - KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (AB113464)
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Supplier Data

Functional Studies - KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (AB113464)

Demonstration of high sensitivity of KDM5/JARID activity assay achieved by using A549 nuclear extracts with ab113464

Key facts

Detection method

Fluorescent

Assay type

Enzyme activity

Assay time

3h

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

KDM5/JARID (Lysine-specific demethylase 5) is a family of histone demethyltransferases involved in H3(meK4) specific demethylation. KDM5/JARID demethylases are JmjC proteins that remove di- and tri-methylation from H3(meK4) but have no activity on mono-methylated residues.

KDM5/JARID Activity Quantification Assay Kit (Fluorometric) (ab113464) is designed for the easy and fast fluorometric measurement of KDM5/JARID activity or inhibition. The antibody-based, immunospecific method directly detects KDM5/JARID-converted demethylated products, rather than by-products, in a 96 stripwell microplate format.

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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab113464-96Test", "assetComponentDetails": [ { "size":"1 x 20 µL", "name":"Fluoro Developer", "number":"AB113464-CMP14" }, { "size":"1 x 60 µL", "name":"Co-factor 1", "number":"AB113464-CMP02" }, { "size":"1 x 1 Unit", "name":"Adhesive Covering Film", "number":"AB113464-CMP05" }, { "size":"1 x 12 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB113464-CMP13" }, { "size":"1 x 8 mL", "name":"JARID Assay Buffer", "number":"AB113464-CMP09" }, { "size":"1 x 60 µL", "name":"Co-factor 2", "number":"AB113464-CMP03" }, { "size":"1 x 60 µL", "name":"Co-factor 3", "number":"AB113464-CMP04" }, { "size":"1 x 28 mL", "name":"10X Wash Buffer", "number":"AB113464-CMP11" }, { "size":"12 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB113464-CMP01" }, { "size":"1 x 120 µL", "name":"JARID Substrate, 50 µg/mL", "number":"AB113464-CMP10" }, { "size":"1 x 8 mL", "name":"Fluoro Dilution", "number":"AB113464-CMP06" }, { "size":"1 x 10 µL", "name":"Capture Antibody, 1000 µg/mL", "number":"AB113464-CMP12" }, { "size":"1 x 20 µL", "name":"Fluoro Enhancer", "number":"AB113464-CMP07" }, { "size":"1 x 20 µL", "name":"JARID Assay Standard, 50 µg/mL", "number":"AB113464-CMP08" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab113464-48Test", "assetComponentDetails": [ { "size":"1 x 10 µL", "name":"Fluoro Developer", "number":"AB113464-CMP14", "productcode":"" }, { "size":"6 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB113464-CMP01", "productcode":"" }, { "size":"1 x 6 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB113464-CMP13", "productcode":"" }, { "size":"1 x 4 mL", "name":"JARID Assay Buffer", "number":"AB113464-CMP09", "productcode":"" }, { "size":"1 x 5 µL", "name":"Capture Antibody, 1000 µg/mL", "number":"AB113464-CMP12", "productcode":"" }, { "size":"1 x 10 µL", "name":"JARID Assay Standard, 50 µg/mL", "number":"AB113464-CMP08", "productcode":"" }, { "size":"1 x 30 µL", "name":"Co-factor 3", "number":"AB113464-CMP04", "productcode":"" }, { "size":"1 x 30 µL", "name":"Co-factor 1", "number":"AB113464-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Adhesive Covering Film", "number":"AB113464-CMP05", "productcode":"" }, { "size":"1 x 60 µL", "name":"JARID Substrate, 50 µg/mL", "number":"AB113464-CMP10", "productcode":"" }, { "size":"1 x 4 mL", "name":"Fluoro Dilution", "number":"AB113464-CMP06", "productcode":"" }, { "size":"1 x 30 µL", "name":"Co-factor 2", "number":"AB113464-CMP03", "productcode":"" }, { "size":"1 x 10 µL", "name":"Fluoro Enhancer", "number":"AB113464-CMP07", "productcode":"" }, { "size":"1 x 14 mL", "name":"10X Wash Buffer", "number":"AB113464-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif (PubMed : 18270511). May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation (PubMed : 19430464). May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1 (By similarity). Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells (PubMed : 27427228).
See full target information KDM5A
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websiteProtocolBooklet
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