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AB156910

KDM6A/ KDM6B Activity Quantification Assay Kit (Colorimetric)

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(3 Publications)

KDM6A/KDM6B Activity Quantification Assay Kit (Colorimetric) (ab156910) is a complete set of optimized reagents, designed for measuring activity/inhibition of KDM6A and KDM6B using nuclear extracts or purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues.

View Alternative Names

UTX, KDM6A, Lysine-specific demethylase 6A, Histone demethylase UTX, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein, [histone H3]-trimethyl-L-lysine(27) demethylase 6A

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Functional Studies - KDM6A/ KDM6B Activity Quantification Assay Kit (Colorimetric) (AB156910)
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Supplier Data

Functional Studies - KDM6A/ KDM6B Activity Quantification Assay Kit (Colorimetric) (AB156910)

Demonstration of high sensitivity of KDM6A/KDM6B activity assay achieved by using KDM6A (UTX) recombinant protein with the KDM6A/KDM6B Activity Quantification Assay Kit (Colorimetric).

Key facts

Detection method

Colorimetric

Sample types

Nuclear Extracts, Purified protein, Cell culture extracts, Tissue Extracts, Cell Lysate

Assay type

Enzyme activity

Sensitivity

> 10000 ng

Range

1 - 20000 ng

Assay time

3h

Assay Platform

Microplate reader

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Reactivity data

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Product details

KDM6A/KDM6B Activity Quantification Assay Kit (Colorimetric) (ab156910) is a complete set of optimized reagents, designed for measuring activity/inhibition of KDM6A and KDM6B using nuclear extracts or purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues. There are currently a limited number of methods used for detecting KDM6A/KDM6B activity/inhibition.

The traditional method is based on the measurement of formaldehyde release, a by-product of KDM6A/KDM6B enzymatic reaction, and has significant weaknesses: (1) large amounts (at μg level) of substrate and enzyme are required; (2) nuclear extracts from cell/tissues cannot be used; (3) redox-sensitive KDM6A/KDM6B inhibitiors are not suitable for testing with such methods; (4) high intereference by SDS, DMSO, thiol-containing chemicals, and ions, which are often contained in enzyme solutions, tested compound solvents, and assay buffers; and (5) less accurate than a direct measurement of KDM6A/KDM6B-converted demethylated products.

KDM6A/KDM6B Activity Quantification Assay Kit (Colorimetric) (ab156910) addresses these issues.

Lysine specific demethylase 6A (KDM6A/UTX) and lysine specific demethylase 6B (KDM6B/JMJD3) demethylase lysine 27 on histone H3, thereby playing a central role in histone code. KDM6A and KDM6B are JmjC (Jumonji)-domain-containing proteins and catalyze the removal of di- and tri-methylation from histone H3-K27 by using a hydroxylation reaction with iron and alpha-glutarate as cofactors.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed : 17713478, PubMed : 17761849, PubMed : 17851529). Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27' (PubMed : 17713478, PubMed : 17761849, PubMed : 17851529). Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed : 17851529). Demethylation of 'Lys-27' of histone H3 is concomitant with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A (PubMed : 17761849). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression (By similarity).
See full target information KDM6A

Additional targets

KDM6B
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 10:780176 PubMed35186918

2022

The Histone H3K27me3 Demethylases KDM6A/B Resist Anoikis and Transcriptionally Regulate Stemness-Related Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed Razeeth Shait Mohammed,Mazin Zamzami,Hani Choudhry,Firoz Ahmed,Bushra Ateeq,Mohammad Imran Khan

Nature metabolism 3:1313-1326 PubMed34650273

2021

Non-canonical glutamine transamination sustains efferocytosis by coupling redox buffering to oxidative phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna Merlin,Stoyan Ivanov,Adélie Dumont,Alexey Sergushichev,Julie Gall,Marion Stunault,Marion Ayrault,Nathalie Vaillant,Alexia Castiglione,Amanda Swain,Francois Orange,Alexandre Gallerand,Thierry Berton,Jean-Charles Martin,Stefania Carobbio,Justine Masson,Inna Gaisler-Salomon,Pierre Maechler,Stephen Rayport,Judith C Sluimer,Erik A L Biessen,Rodolphe R Guinamard,Emmanuel L Gautier,Edward B Thorp,Maxim N Artyomov,Laurent Yvan-Charvet

The Journal of clinical investigation 128:233-247 PubMed29202469

2017

H3K27me3 dynamics dictate evolving uterine states in pregnancy and parturition.

Applications

Unspecified application

Species

Unspecified reactive species

Patrice Nancy,Johan Siewiera,Gabrielle Rizzuto,Elisa Tagliani,Ivan Osokine,Priyanka Manandhar,Igor Dolgalev,Caterina Clementi,Aristotelis Tsirigos,Adrian Erlebacher
View all publications
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