JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB156911

KDM6A/KDM6B Activity Quantification Assay Kit (Fluorometric)

Be the first to review this product! Submit a review

|

(0 Publication)

KDM6A/KDM6B Activity Quantification Assay Kit (Fluorometric) (ab156911) is a complete set of optimized reagents, designed for measuring activity/inhibition of KDM6A and KDM6B using nuclear extracts or purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues.

View Alternative Names

JMJD3, KIAA0346, KDM6B, Lysine-specific demethylase 6B, JmjC domain-containing protein 3, Jumonji domain-containing protein 3, Lysine demethylase 6B, [histone H3]-trimethyl-L-lysine(27) demethylase 6B, UTX, KDM6A, Lysine-specific demethylase 6A, Histone demethylase UTX, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein, [histone H3]-trimethyl-L-lysine(27) demethylase 6A

1 Images
Functional Studies - KDM6A/KDM6B Activity Quantification Assay Kit (Fluorometric) (AB156911)
  • FuncS

Supplier Data

Functional Studies - KDM6A/KDM6B Activity Quantification Assay Kit (Fluorometric) (AB156911)

Typical Results.

Demonstration of high sensitivity of KDM6A/KDM6B activity assay achieved by using KDM6A recombinant protein with the KDM6A/KDM6B Activity Quantification Assay Kit (Fluorometric).

Key facts

Detection method

Fluorescent

Sample types

Nuclear Extracts, Purified protein

Assay type

Enzyme activity

Sensitivity

= 10 ng

Assay time

3h

Assay Platform

Microplate reader

style
left-column

Complementary Products

Assay Kits

AB1432

HDAC Activity Assay Kit (colorimetric)

Enzyme activity assay - HDAC Activity Assay Kit (colorimetric) (AB1432)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Sample Prep & Detection Kits

AB185913

High-Sensitivity ChIP Kit

ChIP - High-Sensitivity ChIP Kit (AB185913)

  • 0
  • 0
Assay Kits

AB115124

Histone H3 Total Acetylation Detection Fast Kit (Colorimetric)

Functional Studies - Histone H3 Total Acetylation Detection Fast Kit (Colorimetric) (AB115124)

  • 4
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

KDM6A/KDM6B Activity Quantification Assay Kit (Fluorometric) (ab156911) is a complete set of optimized reagents, designed for measuring activity/inhibition of KDM6A and KDM6B using nuclear extracts or purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues. There are currently a limited number of methods used for detecting KDM6A/KDM6B activity/inhibition.

The traditional method is based on the measurement of formaldehyde release, a by-product of KDM6A/KDM6B enzymatic reaction, and has significant weaknesses: (1) large amounts (at μg level) of substrate and enzyme are required; (2) nuclear extracts from cell/tissues cannot be used; (3) redox-sensitive KDM6A/KDM6B inhibitiors are not suitable for testing with such methods; (4) high intereference by SDS, DMSO, thiol-containing chemicals, and ions, which are often contained in enzyme solutions, tested compound solvents, and assay buffers; and (5) less accurate than direct measurement of KDM6A/KDM6B-converted demethylated products.

Lysine specific demethylase 6A (KDM6A/UTX) and lysine specific demethylase 6B (KDM6B/JMJD3) demethylase lysine 27 on histone H3, thereby playing a central role in histone code. KDM6A and KDM6B are JmjC (Jumonji)-domain-containing proteins and catalyze the removal of di- and tri-methylation from histone H3-K27 by using a hydroxylation reaction with iron and alpha-glutarate as cofactors.

style
left-column

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab156911-96Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Adhesive Covering Film", "number":"AB156911-CMP05" }, { "size":"1 x 60 µL", "name":"Co-factor 1", "number":"AB156911-CMP02" }, { "size":"1 x 12 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB156911-CMP01" }, { "size":"1 x 120 µL", "name":"Substrate, 50 µg/mL", "number":"AB156911-CMP14" }, { "size":"1 x 8 mL", "name":"Assay Buffer", "number":"AB156911-CMP12" }, { "size":"1 x 8 mL", "name":"Dilution Buffer", "number":"AB156911-CMP09" }, { "size":"1 x 20 µL", "name":"Assay Standard, 50 µg/mL", "number":"AB156911-CMP13" }, { "size":"1 x 10 µL", "name":"Capture Antibody, 1000 µg/mL", "number":"AB156911-CMP07" }, { "size":"1 x 28 mL", "name":"10X Wash Buffer", "number":"AB156911-CMP06" }, { "size":"1 x 12 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB156911-CMP08" }, { "size":"1 x 20 µL", "name":"Fluoro-Enhancer", "number":"AB156911-CMP11" }, { "size":"1 x 60 µL", "name":"Co-factor 2", "number":"AB156911-CMP03" }, { "size":"1 x 60 µL", "name":"Co-factor 3", "number":"AB156911-CMP04" }, { "size":"1 x 20 µL", "name":"Fluoro-Developer", "number":"AB156911-CMP10" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab156911-48Test", "assetComponentDetails": [ { "size":"1 x 4 mL", "name":"Assay Buffer", "number":"AB156911-CMP12", "productcode":"" }, { "size":"1 x 6 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB156911-CMP01", "productcode":"" }, { "size":"1 x 10 µL", "name":"Assay Standard, 50 µg/mL", "number":"AB156911-CMP13", "productcode":"" }, { "size":"1 x 14 mL", "name":"10X Wash Buffer", "number":"AB156911-CMP06", "productcode":"" }, { "size":"1 x 5 µL", "name":"Capture Antibody, 1000 µg/mL", "number":"AB156911-CMP07", "productcode":"" }, { "size":"1 x 6 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB156911-CMP08", "productcode":"" }, { "size":"1 x 10 µL", "name":"Fluoro-Developer", "number":"AB156911-CMP10", "productcode":"" }, { "size":"1 x 30 µL", "name":"Co-factor 2", "number":"AB156911-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Adhesive Covering Film", "number":"AB156911-CMP05", "productcode":"" }, { "size":"1 x 60 µL", "name":"Substrate, 50 µg/mL", "number":"AB156911-CMP14", "productcode":"" }, { "size":"1 x 10 µL", "name":"Fluoro-Enhancer", "number":"AB156911-CMP11", "productcode":"" }, { "size":"1 x 4 mL", "name":"Dilution Buffer", "number":"AB156911-CMP09", "productcode":"" }, { "size":"1 x 30 µL", "name":"Co-factor 3", "number":"AB156911-CMP04", "productcode":"" }, { "size":"1 x 30 µL", "name":"Co-factor 1", "number":"AB156911-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed : 17713478, PubMed : 17825402, PubMed : 17851529, PubMed : 18003914). Demethylates trimethylated and dimethylated H3 'Lys-27' (PubMed : 17713478, PubMed : 17825402, PubMed : 17851529, PubMed : 18003914). Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed : 17851529). Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed : 17825402). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (By similarity).
See full target information KDM6B

Additional targets

KDM6A
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com