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KDM6A/KDM6B Activity Quantification Assay Kit (Fluorometric) (ab156911) is a complete set of optimized reagents, designed for measuring activity/inhibition of KDM6A and KDM6B using nuclear extracts or purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues.

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Images

Functional Studies - KDM6A/KDM6B Activity Quantification Assay Kit (Fluorometric) (AB156911), expandable thumbnail

Key facts

Detection method
Fluorescent
Sample types
Nuclear Extracts, Purified protein
Assay type
Enzyme activity
Reactive species
Plants, Mammals, Fungi
Assay time
3h
Sensitivity
= 10 ng

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed:17713478, PubMed:17825402, PubMed:17851529, PubMed:18003914). Demethylates trimethylated and dimethylated H3 'Lys-27' (PubMed:17713478, PubMed:17825402, PubMed:17851529, PubMed:18003914). Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed:17851529). Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed:17825402). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (By similarity).

Additional Targets

KDM6A

Alternative names

JMJD3, KIAA0346, KDM6B, Lysine-specific demethylase 6B, JmjC domain-containing protein 3, Jumonji domain-containing protein 3, Lysine demethylase 6B, [histone H3]-trimethyl-L-lysine(27) demethylase 6B

What's included?

96 Test
Components
10X Wash Buffer
1 x 28 mL
8-Well Assay Strips (with Frame)
1 x 12 Unit
Adhesive Covering Film
1 x 1 Unit
Assay Buffer
1 x 8 mL
Assay Standard, 50 µg/mL
1 x 20 µL
Capture Antibody, 1000 µg/mL
1 x 10 µL
Co-factor 1
1 x 60 µL
Co-factor 2
1 x 60 µL
Co-factor 3
1 x 60 µL
Detection Antibody, 400 µg/mL
1 x 12 µL
Dilution Buffer
1 x 8 mL
Fluoro-Developer
1 x 20 µL
Fluoro-Enhancer
1 x 20 µL
Substrate, 50 µg/mL
1 x 120 µL

Recommended products

KDM6A/KDM6B Activity Quantification Assay Kit (Fluorometric) (ab156911) is a complete set of optimized reagents, designed for measuring activity/inhibition of KDM6A and KDM6B using nuclear extracts or purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues.

Key facts

Detection method
Fluorescent
Sample types
Nuclear Extracts, Purified protein
Assay type
Enzyme activity
Reactive species
Plants, Mammals, Fungi
Assay time
3h
Assay Platform
Microplate reader
Sensitivity
= 10 ng

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

KDM6A/KDM6B Activity Quantification Assay Kit (Fluorometric) (ab156911) is a complete set of optimized reagents, designed for measuring activity/inhibition of KDM6A and KDM6B using nuclear extracts or purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues. There are currently a limited number of methods used for detecting KDM6A/KDM6B activity/inhibition.

The traditional method is based on the measurement of formaldehyde release, a by-product of KDM6A/KDM6B enzymatic reaction, and has significant weaknesses: (1) large amounts (at μg level) of substrate and enzyme are required; (2) nuclear extracts from cell/tissues cannot be used; (3) redox-sensitive KDM6A/KDM6B inhibitiors are not suitable for testing with such methods; (4) high intereference by SDS, DMSO, thiol-containing chemicals, and ions, which are often contained in enzyme solutions, tested compound solvents, and assay buffers; and (5) less accurate than direct measurement of KDM6A/KDM6B-converted demethylated products.

Lysine specific demethylase 6A (KDM6A/UTX) and lysine specific demethylase 6B (KDM6B/JMJD3) demethylase lysine 27 on histone H3, thereby playing a central role in histone code. KDM6A and KDM6B are JmjC (Jumonji)-domain-containing proteins and catalyze the removal of di- and tri-methylation from histone H3-K27 by using a hydroxylation reaction with iron and alpha-glutarate as cofactors.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Functional Studies - KDM6A/KDM6B Activity Quantification Assay Kit (Fluorometric) (ab156911), expandable thumbnail

    Functional Studies - KDM6A/KDM6B Activity Quantification Assay Kit (Fluorometric) (ab156911)

    Typical Results.

    Demonstration of high sensitivity of KDM6A/KDM6B activity assay achieved by using KDM6A recombinant protein with the KDM6A/KDM6B Activity Quantification Assay Kit (Fluorometric).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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