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AB272541

Ketone Body Assay Kit

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(6 Publications)

Ketone Body Assay Kit ab272541 is a simple, direct and automation-ready procedure for measuring acetoacetic acid (AcAc) and 3-hydroxybutyric acid (BOH).

We recommend Ketone Body Assay Kit ab318308, which was developed as a replacement for this kit.
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Functional Studies - Ketone Body Assay Kit (AB272541)
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Supplier Data

Functional Studies - Ketone Body Assay Kit (AB272541)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Example of Acetoacetic Acid (AcAc) and 3-Hydroxybutyric Acid (BOH) standard curve.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum, Other biological fluids

Results type

Quantitative

Range

0.12 - 8 mM

Assay Platform

Microplate

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Product details

Ketone Body Assay Kit (ab272541) is a simple, direct and automation-ready procedure for measuring acetoacetic acid (AcAc) and 3-hydroxybutyric acid (BOH). This ketone body assay is based on 3-hydroxybutyrate dehydrogenase catalyzed reactions, in which the change in NADH absorbance, measured at 340nm, is directly related to the AcAc and BOH concentrations.

Sensitive and accurate: Uses 10 μL sample. Linear detection range of 0.12 to 8 mM for each ketone body in 96-well plate assay.

Convenient: The procedure involves adding a single working reagent, and reading the optical density at room temperature.

High-throughput: Can be automated as a high-throughput 96-well plate assay for many samples per day.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ketone bodies sometimes referred to as ketones are small lipid-derived molecules including acetoacetate beta-hydroxybutyrate and acetone each with specific molecular masses. These molecules are produced in the liver during the breakdown of fatty acids and are soluble in water. Ketone bodies transport energy from the liver to other tissues particularly when glucose levels are low. They serve as an alternative energy source for almost all organ systems including the heart and brain and are detectable using ketone test kits or ketosis test kits.
Biological function summary

Ketone bodies function as a significant fuel source during prolonged fasting carbohydrate restriction or strenuous exercise. They do not typically exist as part of a complex but instead circulate freely in the bloodstream. Ketone bodies can cross the blood-brain barrier to supply energy to the brain when glucose is scarce. Their production and utilization are part of normal metabolic processes aimed at maintaining energy homeostasis especially under conditions where glucose availability is limited.

Pathways

Ketone bodies are integral to the ketogenic metabolic pathway which is important in energy metabolism during periods of low carbohydrate intake. They are interlinked with the fatty acid oxidation pathway an important process in energy production. Specific proteins like carnitine palmitoyltransferase I (CPT1) regulate the entry of fatty acids into the mitochondria for oxidation further connecting to ketone synthesis. Similarly the enzyme HMG-CoA lyase is vital during the ketogenesis process highlighting ketone bodies' involvement in metabolic pathways.

Ketone bodies have a significant connection to diabetic ketoacidosis and epilepsy. In diabetic ketoacidosis a dangerous accumulation of ketone bodies occurs due to inadequate insulin action leading to acidosis. The enzyme insulin which normally helps in glucose uptake and utilization plays a central role in this disorder. In epilepsy a ketogenic diet that promotes ketone body production helps manage seizures. The diet's metabolic influence on neurons involves alterations in neurotransmitter levels and energy substrate availability.
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Product protocols

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Target data

See full target information Acetoacetic acid
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of food and drug analysis 33:48-60 PubMed40202407

2025

Protective role of Hsian-tsao ethanol extract against body fat, serum lipid profiles, and hepatic lipid profiles in high-fat diet-fed rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Chang Cho,Gow-Chin Yen,Hsin-Yi Lee,Chun-Tse Tsai,Wei-Tang Chang,Chin-Lin Hsu

eLife 13: PubMed39886919

2025

Role of hepatocyte RIPK1 in maintaining liver homeostasis during metabolic challenges.

Applications

Unspecified application

Species

Unspecified reactive species

Weigao Zhang,Hu Liu,Danyang Zhang,Yuguo Yi,Liang Tao,Yunfeng Zhu,Shuxian Huang,Xunan Zhao,Qianchao Shao,Peiqi Li,Yiwen Weng,Wei Lu,Jianfa Zhang,Haibing Zhang,Yuxin Chen,Dan Weng

Nutrients 16: PubMed38794639

2024

Ketogenic Diet Induced Shifts in the Gut Microbiome Associate with Changes to Inflammatory Cytokines and Brain-Related miRNAs in Children with Autism Spectrum Disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Nina P Allan,Brennan Y Yamamoto,Braden P Kunihiro,Chandler K L Nunokawa,Noelle C Rubas,Riley K Wells,Lesley Umeda,Krit Phankitnirundorn,Amada Torres,Rafael Peres,Emi Takahashi,Alika K Maunakea

Pharmaceutics 14: PubMed36559124

2022

Combined Therapy of Low-Dose Angiotensin Receptor-Neprilysin Inhibitor and Sodium-Glucose Cotransporter-2 Inhibitor Prevents Doxorubicin-Induced Cardiac Dysfunction in Rodent Model with Minimal Adverse Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Donghyun Kim,Gyuho Jang,Jaetaek Hwang,Xiaofan Wei,Hyunsoo Kim,Jinbae Son,Sang-Jae Rhee,Kyeong-Ho Yun,Seok-Kyu Oh,Chang-Myung Oh,Raekil Park

Cells 11: PubMed36230949

2022

2-Deoxy-D-glucose Alleviates Cancer Cachexia-Induced Muscle Wasting by Enhancing Ketone Metabolism and Inhibiting the Cori Cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Wei,Ranran Wang,Junaid Wazir,Kai Lin,Shiyu Song,Li Li,Wenyuan Pu,Chen Zhao,Yong Wang,Zhonglan Su,Hongwei Wang

Cellular and molecular life sciences : CMLS 79:265 PubMed35484438

2022

Impaired regulation of PMCA activity by defective CFTR expression promotes epithelial cell damage in alcoholic pancreatitis and hepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara Madácsy,Árpád Varga,Noémi Papp,Bálint Tél,Petra Pallagi,Viktória Szabó,Aletta Kiss,Júlia Fanczal,Zoltan Rakonczay,László Tiszlavicz,Zsolt Rázga,Meike Hohwieler,Alexander Kleger,Mike Gray,Péter Hegyi,József Maléth
View all publications
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Product promise

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