Kinetic Apoptosis Kit (Microscopy)

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AbReview61645****

Functional Studies - Kinetic Apoptosis Kit (Microscopy) (AB129817)

Kinetic Apoptosis and Necrosis Analysis of HT1080 cells.

HT1080 cells were plated at a density of 2000 cells/well in 96 well plates and allowed to adhere overnight. The next day, cells were treated with dilutions of camptothecin, starting at 2500 nM, in order to determine the effect of camptothecin on the apoptotic and necrotic response of HT1080 cells. Our results show an increase over time in the percentage of GFP positive apoptotic cells (A) and PI positive necrotic cells (B). Apoptotic and necrotic cells are normalized to high contrast bright field cell counts. This increase is dependent upon concentrations of camptothecin. The accompanying figure also shows a dose response curve for apoptosis (C) and necrosis (D), demonstrating that both the apoptotic and necrotic response show a dose dependent increase following treatment with camptothecin. The dose response curve is based on 48 hours of camptothecin treatment.

This image is courtesy of an AbReview submitted by Sarah Beckman.

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Functional Studies - Kinetic Apoptosis Kit (Microscopy) (AB129817)

Time-lapse images showing the progressive movement of PS exposure along axons to the cell body as detected by Kinetic Apoptosis Kit (pSIVA-IANBD). Images were taken 10-14 h (3 frames/h) after NGF removal. *Indicates where PI staining was first seen in the cell body. Black and white : pSIVA-IANBD fluorescence. Bottom panel : Merged images of phase contrast, green (pSIVA-IANBD) and red (PI) fluorescence. 100 μm scale bar. Cells were imaged with pSIVA-IANBD and PI present in the culture medium for the duration of the experiment. Figure from Kim et al 2010a.