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- Enzyme activity assay
Unknown
Enzyme activity assay - L-Arginine Assay Kit (AB241028)
Estimations of L-arginine in two human serum sample (30 µL) and orange juice (1.6 µL).
Estimations of L-arginine in two human serum sample (30 μL) and orange juice (1.6 μL). L-arginine concentrations were 0.278 mM and 0.069 mM in the two human serum samples, and 5.113 mM in orange juice. Assays were performed following the kit protocol.
- Enzyme activity assay
Unknown
Enzyme activity assay - L-Arginine Assay Kit (AB241028)
Specificity of the detection of L-arginine over other amino acids : D-arginine and other L-amino acids were tested at a 2-fold molar excess (each AA : 40 nmol) vs L-arginine (20 nmol).
- Enzyme activity assay
Unknown
Enzyme activity assay - L-Arginine Assay Kit (AB241028)
L-arginine standard curve.
Product details
L-Arginine Assay Kit (ab241028) provides a quick, specific, and easy method for the measurement of total L-arginine concentrations in a wide variety of samples. In this enzyme-based assay, L-arginine is converted into a series of intermediates, which will further react with a probe producing a stable colorimetric signal (OD: 450 nm).
The kit is simple to use, sensitive and high-throughput adaptable and can detect as low as 1 nmol/well of L-arginine in biological samples.
Arginine assay protocol summary
- add samples and standards to wells - add enzyme mix and background mix, and incubate for 30-min at 37°C - add reaction mix, and incubate for 60-min at 37°C - analyse with plate reader
Other notes
This product was previously called K749 Biovision L-Arginine Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Publications (5)
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Bioactive materials 40:1-18 PubMed38873262
2024
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Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2307256 PubMed38233193
2024
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Frontiers in endocrinology 14:1252727 PubMed37810891
2023
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Oxidative medicine and cellular longevity 2021:5510663 PubMed33791071
2021
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Communications biology 3:611 PubMed33097830
2020
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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