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AB83392

L-Carnitine Assay Kit

4

(1 Review)

|

(6 Publications)

L-Carnitine Assay Kit ab83392 is a simple convenient means of measuring free L-Carnitine in biological samples such as serum.

View Alternative Names

Organic anion transporter 3, S22A8_HUMAN, SLC22A8, Solute carrier family 22 (organic anion transporter), member 8, Solute carrier family 22 member 8, hOAT3

3 Images
Functional Studies - L-Carnitine Assay Kit (AB83392)
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Supplier Data

Functional Studies - L-Carnitine Assay Kit (AB83392)

Example of L-Carnitine standard curve obtained using ab83392.

Functional Studies - L-Carnitine Assay Kit (AB83392)
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Lab

Functional Studies - L-Carnitine Assay Kit (AB83392)

Functional assay - 83392 L-Carnitine Assay Kit.

L-Carnitine measured in biologicals showing concentration (micromolar).

Functional Studies - L-Carnitine Assay Kit (AB83392)
  • FuncS

Lab

Functional Studies - L-Carnitine Assay Kit (AB83392)

Functional assay - 83392 L-Carnitine Assay Kit.

Fluorimetric standard curve : mean of duplicates (+/- SD) with background readings subtracted.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Tissue Lysate, Urine, Plasma, Serum, Other biological fluids, Cell Lysate

Assay type

Quantitative

Range

1 - 200 µM

Assay time

30m

Assay Platform

Microplate reader

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Product details

L-Carnitine Assay Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as serum. The assay transfers an acetyl group from CoA to carnitine and the free CoA formed is further processed with subsequent oxidation of the OxiRed probe to give fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). The normal range for serum L-Carnitine is ~20-100 uM. The detection sensitivity is ~1 uM for the fluorometric assay and ~10 uM for the colorimetric assay.

Other Notes
This product was previously called K642 L-Carnitine Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Carnitine is a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine. It is required for transport of fatty acids into the mitochondrial matrix via the carnitine/acylcarnitine shuttle where beta-oxidation occurs, acetate is generated and the acetate utilized in the TCA cycle for the generation of energy. L-Carnitine is often sold as a nutritional supplement. Carnitine exists in two stereoisomers. Only L-carnitine is biologically active.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab83392-100Test", "assetComponentDetails": [ { "size":"1 x 400 µL", "name":"Carnitine Substrate Mix", "number":"AB83392-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Carnitine Standard", "number":"AB83392-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Carnitine Development Mix", "number":"AB83392-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Carnitine Converting Enzyme", "number":"AB83392-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Carnitine Assay Buffer", "number":"AB83392-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"OxiRed™ Probe", "number":"AB83392-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

L-Carnitine also known as levocarnitine is a quaternary ammonium compound involved in metabolism with an approximate mass of 161.2 g/mol. It facilitates the transport of fatty acids into the mitochondria for beta-oxidation serving a critical role as part of the carnitine shuttle. L-Carnitine is expressed predominantly in tissues with high metabolic activity including heart and skeletal muscles. The compound closely relates to the carnitine acetyltransferase enzyme which aids in the transport of acetyl groups across biological membranes.
Biological function summary

L-Carnitine enhances fatty acid oxidation improving energy production within cells. This compound functions independently not forming part of a protein complex. It also helps maintain the acetyl-CoA to CoA ratio within cells influencing various metabolic reactions. By facilitating the removal of short-chain and medium-chain fatty acids from mitochondria L-Carnitine supports the cell's energy homeostasis and prevents the accumulation of potentially harmful acyl-CoA species.

Pathways

L-Carnitine plays a vital role in energy metabolism and fatty acid catabolism. One key pathway it participates in is the fatty acid oxidation pathway also known as beta-oxidation. Within this pathway L-Carnitine works with enzymes such as carnitine palmitoyltransferase I and II to transport long-chain fatty acids into the mitochondrial matrix. Another pathway involves ketogenesis in the liver where L-Carnitine aids in converting fatty acids into ketones during fasting states stabilizing energy production.

L-Carnitine deficiency links to conditions like cardiomyopathy and skeletal muscle weakness due to its essential role in muscle metabolism. A deficiency may also contribute to disorders such as systemic primary carnitine deficiency impacting heart and skeletal muscles due to impaired fatty acid transport. Associations with proteins such as carnitine transporters (OCTN2) show how a malfunction can lead to metabolic disturbances highlighting the importance of L-Carnitine in maintaining metabolic function.
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Product protocols

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Target data

See full target information Levocarnitine
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1543 PubMed39934163

2025

BBOX1 restrains TBK1-mTORC1 oncogenic signaling in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chengheng Liao,Lianxin Hu,Liwei Jia,Jin Zhou,Tao Wang,Kangsan Kim,Hua Zhong,Hongwei Yao,Lei Dong,Lei Guo,Qian Liang,Cheng Zhang,Fangzhou Zhao,Jun Fang,Hongyi Liu,Shina Li,Lin Xu,Jeremy M Simon,Srinivas Malladi,Payal Kapur,James Brugarolas,Ralph J DeBerardinis,Qing Zhang

Cellular and molecular life sciences : CMLS 82:13 PubMed39719538

2024

Trimethylamine-N-oxide accelerates osteoporosis by PERK activation of ATF5 unfolding.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Han Lin,Wei-Shiung Lian,Re-Wen Wu,Yu-Shan Chen,Shin-Long Wu,Jih-Yang Ko,Shao-Yu Wang,Holger Jahr,Feng-Sheng Wang

Cell death & disease 15:385 PubMed38824126

2024

PGC-1α repression dysregulates lipid metabolism and induces lipid droplet accumulation in retinal pigment epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyan Zhou,Kaan Taskintuna,Jacob Hum,Jasmine Gulati,Stephanie Olaya,Jeremy Steinman,Nady Golestaneh

PloS one 19:e0298942 PubMed38625934

2024

Domestic dogs maintain clinical, nutritional, and hematological health outcomes when fed a commercial plant-based diet for a year.

Applications

Unspecified application

Species

Unspecified reactive species

Annika Linde,Maureen Lahiff,Adam Krantz,Nathan Sharp,Theros T Ng,Tonatiuh Melgarejo

Pulmonary pharmacology & therapeutics 65:101998 PubMed33556627

2021

Comparison of l-Carnitine and l-Carnitine HCL salt for targeted lung treatment of pulmonary hypertension (PH) as inhalation aerosols: Design, comprehensive characterization, in vitro 2D/3D cell cultures, and in vivo MCT-Rat model of PH.

Applications

Unspecified application

Species

Unspecified reactive species

Maria F Acosta,Priya Muralidhran,Michael D Abrahamson,Carissa L Grijalva,Megan Carver,Haiyang Tang,Christina Klinger,Jeffrey R Fineman,Stephen M Black,Heidi M Mansour

Scientific reports 6:20299 PubMed26831370

2016

Longitudinal metabolic imaging of hepatocellular carcinoma in transgenic mouse models identifies acylcarnitine as a potential biomarker for early detection.

Applications

Unspecified application

Species

Unspecified reactive species

Jadegoud Yaligar,Wei Wei Teoh,Rashidah Othman,Sanjay Kumar Verma,Beng Hooi Phang,Swee Shean Lee,Who Whong Wang,Han Chong Toh,Venkatesh Gopalan,Kanaga Sabapathy,S Sendhil Velan
View all publications
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