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L-Carnitine Assay Kit ab83392 is a simple convenient means of measuring free L-Carnitine in biological samples such as serum.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.


Images

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Tissue Lysate, Urine, Plasma, Serum, Other biological fluids, Cell Lysate

Assay type

Quantitative

Reactive species

Mammals

Range

1 - 200 µM

Assay time

30m

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Alternative names

What's included?

100 Test
Components
Carnitine Assay Buffer
1 x 25 mL
Carnitine Converting Enzyme
1 x 1 Vial
Carnitine Development Mix
1 x 1 Vial
Carnitine Standard
1 x 1 Vial
Carnitine Substrate Mix
1 x 400 µL
OxiRed Probe
1 x 200 µL

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L-Carnitine Assay Kit ab83392 is a simple convenient means of measuring free L-Carnitine in biological samples such as serum.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Alternative names

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Tissue Lysate, Urine, Plasma, Serum, Other biological fluids, Cell Lysate

Assay type

Quantitative

Reactive species

Mammals

Range

1 - 200 µM

Assay time

30m

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Abcam's L-Carnitine Assay Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as serum. The assay transfers an acetyl group from CoA to carnitine and the free CoA formed is further processed with subsequent oxidation of the OxiRed probe to give fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). The normal range for serum L-Carnitine is ~20-100 μM. The detection sensitivity is ~1 μM for the fluorometric assay and ~10 μM for the colorimetric assay.
Visit our FAQs page for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called K642 L-Carnitine Colorimetric/Fluorometric Assay Kit. K642-100 is the same size as the 100 test size of ab83392.

Carnitine is a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine. It is required for transport of fatty acids into the mitochondrial matrix via the carnitine/acylcarnitine shuttle where beta-oxidation occurs, acetate is generated and the acetate utilized in the TCA cycle for the generation of energy. L-Carnitine is often sold as a nutritional supplement. Carnitine exists in two stereoisomers. Only L-carnitine is biologically active.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Functional Studies - L-Carnitine Assay Kit (ab83392), expandable thumbnail

    Functional Studies - L-Carnitine Assay Kit (ab83392)

    Functional assay - 83392 L-Carnitine Assay Kit.

    Fluorimetric standard curve: mean of duplicates (+/- SD) with background readings subtracted.

  • Functional Studies - L-Carnitine Assay Kit (ab83392), expandable thumbnail

    Functional Studies - L-Carnitine Assay Kit (ab83392)

    Functional assay - 83392 L-Carnitine Assay Kit.

    L-Carnitine measured in biologicals showing concentration (micromolar).

  • Functional Studies - L-Carnitine Assay Kit (ab83392), expandable thumbnail

    Functional Studies - L-Carnitine Assay Kit (ab83392)

    Example of L-Carnitine standard curve obtained using ab83392.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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