L-Lactate Assay Kit ab65331 is a mix-and-read, no-wash assay with one 30 minute incubation. In the assay, lactate is oxidized by lactate dehydrogenase, with a colorimetric assay readout (450 nm).
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Environmental toxicology 39:3304-33132024lncRNA NORAD alleviates dysfunction of renal proximal tubular epithelial cells during the sepsis-associated acute kidney injury by modulating the miR-155-5p-PDK1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHulin Lu,Yan Chen,Yong Yang,Min Ding,Fengping QiuPubMed 38433477
- Pediatric pulmonology 59:1266-12732024Effects of highly effective modulator therapy on the dynamics of the respiratory mucosal environment and inflammatory response in cystic fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamar E Atteih,Catherine R Armbruster,Yasmin Hilliam,Glenn J Rapsinski,Junu Koirala Bhusal,Leah L Krainz,Jordan R Gaston,Matthew DuPont,Anna C Zemke,John F Alcorn,John A Moore,Vaughn S Cooper,Stella E Lee,Erick Forno,Jennifer M BombergerPubMed 38353361
- Cell communication and signaling : CCS 22:512024NAT10 mediated ac4C acetylation driven mA modification via involvement of YTHDC1-LDHA/PFKM regulates glycolysis and promotes osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongting Mei,Zhihua Shen,Jiaying Pu,Qian Liu,Guoxin Liu,Xuting He,Yang Wang,Jinrui Yue,Shiyu Ge,Tao Li,Ye Yuan,Lei YangPubMed 38233839
- Breast cancer research : BCR 26:102024PFKP deubiquitination and stabilization by USP5 activate aerobic glycolysis to promote triple-negative breast cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZi-Mei Peng,Xiao-Jian Han,Tao Wang,Jian-Jun Li,Chun-Xi Yang,Fang-Fang Tou,Zhen ZhangPubMed 38217030
- Scientific reports 14:8452024Radiation-induced changes in energy metabolism result in mitochondrial dysfunction in salivary glands.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLauren G Buss,Brenna A Rheinheimer,Kirsten H LimesandPubMed 38191641
- Scientific reports 13:224062023Mass spectrometry in cerebrospinal fluid uncovers association of glycolysis biomarkers with Alzheimer's disease in a large clinical sample.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthijs B de Geus,Shannon N Leslie,TuKiet Lam,Weiwei Wang,Florence Roux-Dalvai,Arnaud Droit,Pia Kivisakk,Angus C Nairn,Steven E Arnold,Becky C CarlylePubMed 38104170
- Journal of extracellular vesicles 12:e123872023A clinically relevant large-scale biomanufacturing workflow to produce natural killer cells and natural killer cell-derived extracellular vesicles for cancer immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrederic St-Denis-Bissonnette,Sarah E Cummings,Shirley Qiu,Andrew Stalker,Gauri Muradia,Jelica Mehic,Karan Mediratta,Shelby Kaczmarek,Dylan Burger,Seung-Hwan Lee,Lisheng Wang,Jessie R LavoiePubMed 38054534
- Physiological reports 11:e158672023Cooling of male rat skeletal muscle during endurance-like contraction attenuates contraction-induced PGC-1α mRNA expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaisuke Hoshino,Ryota Wada,Yutaro Mori,Reo Takeda,Yudai Nonaka,Ryotaro Kano,Ryo Takagi,Yutaka KanoPubMed 37962014
- eLife 12:2023Astrocyte and L-lactate in the anterior cingulate cortex modulate schema memory and neuronal mitochondrial biogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMastura Akter,Mahadi Hasan,Aruna Surendran Ramkrishnan,Zafar Iqbal,Xianlin Zheng,Zhongqi Fu,Zhuogui Lei,Anwarul Karim,Ying LiPubMed 37960975
- Cell reports. Medicine 4:1012642023Targeting the TCA cycle through cuproptosis confers synthetic lethality on ARID1A-deficient hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Xing,Li Li,Yiran Chen,Gaoda Ju,Guilan Li,Xiaoyun Zhu,Yubo Ren,Jing Zhao,Zhilei Cheng,Yan Li,Da Xu,Jun LiangPubMed 37939712
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Range
0.02 - 10 mM
- Assay time
30m
- Sensitivity
= 0.02 mM
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L-Lactate Assay Kit ab65331 is a mix-and-read, no-wash assay with one 30 minute incubation. In the assay, lactate is oxidized by lactate dehydrogenase, with a colorimetric assay readout (450 nm).
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Colorimetric
- Sample types
Tissue Lysate, Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Range
0.02 - 10 mM
- Assay time
30m
- Assay Platform
Microplate reader
- Sensitivity
= 0.02 mM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
L-Lactate Assay Kit (Colorimetric) (ab65331) uses an assay protocol where lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a probe to produce a color (λmax = 450 nm).
The kit detects L(+)-Lactate in biological samples such as serum or plasma, cells, tissues, cell culture and fermentation media.
Lactate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader
L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present (see ) but only at about 1-5% of the concentration of L(+)-Lactate. L-Lactate assay kit ab65331 is our most popular L-Lactate assay kit (colorimetric 450nm, range 0.02 mM - 10 mM). Alternative L-Lactate assay kits offer different readout modes/wavelengths and sensitivity/range: - : colorimetric 570 nm, fluorometric Ex/Em 535/587 nm, range 0.001 mM - 10 mM - : fluorometric Ex/Em 535/587 nm, range 0.2 µM - 50 µM Review our to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader. **How other researchers have used L-Lactate Assay Kit ab65331** This Lactate assay kit has been used in publications in a variety of sample types, including: - Human: THP-1 cell lysates1, MDA-MB-231 and HepG2 cell culture lysates2, cell culture supernatant (HepG2, A549, Huh7, PC3, LN229, HeLa)3, brain tissue4 - Mouse: brown adipose tissue lysate5, thymic lymphoma tissue6, cell culture supernatant7, T cell primary cell culture supernatants8, serum9, serum and muscle10 - Bovine: cumulus cell culture supernatant11 References: 1 - Tran UT and Kitami T 2019; 2 - Cui J et al 2019; 3 - Rodriguez ML et al 2018, Chen Y et al 2018, Zhang D et al 2018, Caino MC et al 2017, Birkenmeier K et al 2015; 4 - Sullivan RC et al 2019; 5 - Jeong JH et al 2018; 6 - Vara-Ciruelos D et al 2019; 7 - Fiorenzano et al 2016; 8 - Menk AV et al 2018; 9 - Deng W et al 2019, Guglielmetti C et al 2017, Kang R et al 2016; 10 - Kim HY et al 2016; 11 - Sinha et al 2017
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
L-Lactate also known as lactate is a byproduct of anaerobic glycolysis where it plays an important role in energy metabolism. L-Lactate is a small molecule with a molecular mass of approximately 90.08 g/mol. It forms in various tissues like muscle cells during intense exercise when oxygen levels are low. This process leads to a conversion of pyruvate to lactate by the action of the enzyme lactate dehydrogenase (LDH) which is present in many tissues with higher expressions in muscles and heart.
- Biological function summary
L-Lactate acts as a signaling molecule which affects cellular functions and contributes to metabolic regulation. It is not part of a complex but serves as an important intermediate in metabolic pathways. L-Lactate provides energy to cells by converting back to pyruvate in the presence of oxygen which then enters the citric acid cycle. This conversion and its utilization as energy play important roles in balancing cellular energy demands especially under hypoxic conditions.
- Pathways
L-Lactate links to critical processes like glycolysis and the Cori cycle. During glycolysis pyruvate may convert to L-Lactate under anaerobic conditions to regenerate NAD+ necessary for glycolysis to continue. In the Cori cycle lactate produced by anaerobic glycolysis in muscles is released into the bloodstream and transported to the liver. There it converts back to glucose supporting gluconeogenesis. These processes highlight the close involvement of L-Lactate with proteins such as lactate dehydrogenase and pyruvate kinase.
- Associated diseases and disorders
L-Lactate is associated with conditions like lactic acidosis and cancer. Lactic acidosis characterized by high lactate levels can occur due to oxygen deprivation or mitochondrial dysfunction. Meanwhile cancer cells often show enhanced glycolysis and lactate production known as the Warburg effect facilitating their growth. Proteins like hypoxia-inducible factor 1-alpha (HIF-1α) and lactate dehydrogenase (LDH) are key players in these conditions influencing lactate metabolism and potentially serving as therapeutic targets.
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5 product images
Image from Kelly CE et al., PLoS Biol 11(4), fig2d. oi: 10.1371/journal.pbio.1001538. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - L-Lactate Assay Kit (Colorimetric) (ab65331)
Plasma lactate concentrations were determined using L-Lactate assay kit (ab65331) in Ark2C+/+ and Ark2C−/− (Arkadia-like gene) mice.
Functional Studies - L-Lactate Assay Kit (Colorimetric) (ab65331)
Linearity of dilution: concentration of L-Lactate in differently diluted (X-axis) biological samples, demonstrating a linearity of 89%-111% (concentrations corrected for by factor of dilution; duplicates; +/- SD).
Functional Studies - L-Lactate Assay Kit (Colorimetric) (ab65331)
Relative signal (RFU) in unfiltered human plasma (dilution 1:8), comparing L-lactate signals with background reading (no enzyme) after 10 minutes of incubation (duplicates +/- SD).
Functional Studies - L-Lactate Assay Kit (Colorimetric) (ab65331)
Standard curve with background signal subtracted (duplicates; +/- SD).
Functional Studies - L-Lactate Assay Kit (Colorimetric) (ab65331)
Lactate Standard Curve. The assay is performed following the kit (ab65331) protocol.
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