L-Lactate Assay Kit (Colorimetric)

L-Lactate Assay Kit (Colorimetric) (ab65331) uses an assay protocol where lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a probe to produce a color (λmax = 450 nm).

The kit detects L(+)-Lactate in biological samples such as serum or plasma, cells, tissues, cell culture and fermentation media.

Lactate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader

L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present (see ) but only at about 1-5% of the concentration of L(+)-Lactate. L-Lactate assay kit ab65331 is our most popular L-Lactate assay kit (colorimetric 450nm, range 0.02 mM - 10 mM). Alternative L-Lactate assay kits offer different readout modes/wavelengths and sensitivity/range: - : colorimetric 570 nm, fluorometric Ex/Em 535/587 nm, range 0.001 mM - 10 mM - : fluorometric Ex/Em 535/587 nm, range 0.2 µM - 50 µM Review our to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader. **How other researchers have used L-Lactate Assay Kit ab65331** This Lactate assay kit has been used in publications in a variety of sample types, including: - Human: THP-1 cell lysates1, MDA-MB-231 and HepG2 cell culture lysates2, cell culture supernatant (HepG2, A549, Huh7, PC3, LN229, HeLa)3, brain tissue4 - Mouse: brown adipose tissue lysate5, thymic lymphoma tissue6, cell culture supernatant7, T cell primary cell culture supernatants8, serum9, serum and muscle10 - Bovine: cumulus cell culture supernatant11 References: 1 - Tran UT and Kitami T 2019; 2 - Cui J et al 2019; 3 - Rodriguez ML et al 2018, Chen Y et al 2018, Zhang D et al 2018, Caino MC et al 2017, Birkenmeier K et al 2015; 4 - Sullivan RC et al 2019; 5 - Jeong JH et al 2018; 6 - Vara-Ciruelos D et al 2019; 7 - Fiorenzano et al 2016; 8 - Menk AV et al 2018; 9 - Deng W et al 2019, Guglielmetti C et al 2017, Kang R et al 2016; 10 - Kim HY et al 2016; 11 - Sinha et al 2017