L-Lactate Assay Kit (Colorimetric/Fluorometric) ab65330 provides a convenient means for detecting L(+)-Lactate in biological samples such as blood, cells, culture mediums, fermentation mediums, etc.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Nature communications 14:78892023The poxvirus F17 protein counteracts mitochondrially orchestrated antiviral responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNathan Meade,Helen K Toreev,Ram P Chakrabarty,Charles R Hesser,Chorong Park,Navdeep S Chandel,Derek WalshPubMed 38036506
- International journal of molecular sciences 24:2023Impact of NLRP3 Depletion on Aging-Related Metaflammation, Cognitive Function, and Social Behavior in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena D Khilazheva,Angelina I Mosiagina,Yulia A Panina,Olga S Belozor,Yulia K KomlevaPubMed 38068904
- PLoS pathogens 19:e10117192023Interferon signaling drives epithelial metabolic reprogramming to promote secondary bacterial infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGrace P Carreno-Florez,Brian R Kocak,Matthew R Hendricks,Jeffrey A Melvin,Katrina B Mar,Jessica Kosanovich,Rachel L Cumberland,Greg M Delgoffe,Sruti Shiva,Kerry M Empey,John W Schoggins,Jennifer M BombergerPubMed 37939149
- Environmental toxicology 39:1269-12822023SNHG1, interacting with SND1, contributes to sorafenib resistance of liver cancer cells by increasing m6A-mediated SLC7A11 expression and promoting aerobic glycolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRong Li,Shunle Li,Lin Shen,Junhui Li,Di Zhang,Jinmin Yu,Lanxuan Huang,Na Liu,Hongwei Lu,Meng XuPubMed 37927237
- Journal of the European Academy of Dermatology and Venereology : JEADV 37:2067-20792023Recombinant Treponema pallidum protein Tp47 promoted the phagocytosis of macrophages by activating NLRP3 inflammasome induced by PKM2-dependent glycolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesY-W Zheng,M Wang,J-W Xie,R Chen,X-T Wang,Y He,T-C Yang,L-L Liu,L-R LinPubMed 37247195
- Cells 12:2023Corneal Edema in Inducible Knockout Is Initiated by Mitochondrial Superoxide Induced Src Kinase Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiego G Ogando,Edward T Kim,Shimin Li,Joseph A BonannoPubMed 37296649
- The FEBS journal 290:4480-44952023Metformin exerts antileukemic effects by modulating lactate metabolism and overcomes imatinib resistance in chronic myelogenous leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeher Bolisetti Gayatri,Rama Krishna Kancha,Dorababu Patchva,Nagaraj Velugonda,Sadashivudu Gundeti,Aramati B M ReddyPubMed 37171230
- Applied biochemistry and biotechnology 195:5217-52372023Salvigenin Suppresses Hepatocellular Carcinoma Glycolysis and Chemoresistance Through Inactivating the PI3K/AKT/GSK-3β Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Shao,Jingyan Chen,Ali Li,Lili Ma,Yongzhi Tang,Huazhong Chen,Yongping Chen,Junyan LiuPubMed 37129745
- Nature 617:386-3942023A druggable copper-signalling pathway that drives inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStéphanie Solier,Sebastian Müller,Tatiana Cañeque,Antoine Versini,Arnaud Mansart,Fabien Sindikubwabo,Leeroy Baron,Laila Emam,Pierre Gestraud,G Dan Pantoș,Vincent Gandon,Christine Gaillet,Ting-Di Wu,Florent Dingli,Damarys Loew,Sylvain Baulande,Sylvère Durand,Valentin Sencio,Cyril Robil,François Trottein,David Péricat,Emmanuelle Näser,Céline Cougoule,Etienne Meunier,Anne-Laure Bègue,Hélène Salmon,Nicolas Manel,Alain Puisieux,Sarah Watson,Mark A Dawson,Nicolas Servant,Guido Kroemer,Djillali Annane,Raphaël RodriguezPubMed 37100912
- Microbiology spectrum 11:e00909232023in Staphylococcus aureus Contributes to Microaerobic Metabolic Activity, Abscess Formation, and Survival in Macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengning Chen,Yuyao Yin,Hongbin Chen,Shuguang Li,Guankun Yin,Hui WangPubMed 37052483
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Tissue Lysate, Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Reactive species
Mammals
- Range
0.001 - 10 mM
- Assay time
40m
- Sensitivity
> 0.001 mM
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L-Lactate Assay Kit (Colorimetric/Fluorometric) ab65330 provides a convenient means for detecting L(+)-Lactate in biological samples such as blood, cells, culture mediums, fermentation mediums, etc.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Tissue Lysate, Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Reactive species
Mammals
- Range
0.001 - 10 mM
- Assay time
40m
- Assay Platform
Microplate reader
- Sensitivity
> 0.001 mM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
L-Lactate Assay Kit (Colorimetric/Fluorometric) ab65330 provides a convenient means for detecting L(+)-Lactate in biological samples such as blood, cells, culture mediums, fermentation mediums, etc. There is no need for pretreatment or purification of samples.
In the lactate assay protocol, lactate specifically reacts with an enzyme mix to generate a product, which interacts with a lactate probe to produce color (570 nm) and fluorescence (at Ex/Em = 535/587 nm).
Lactate assay protocol summary:
- add samples (deproteinized) and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader
L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present (see ) but only at about 1-5% of the concentration of L(+)-Lactate. Alternative L-Lactate assay kits offer different readout modes/wavelengths and sensitivity/range: - this kit L-Lactate assay ab65330: colorimetric 570 nm, fluorometric Ex/Em 535/587 nm, range 0.001 mM - 10 mM - our most popular : colorimetric 450nm, range 0.02 mM - 10 mM - : fluorometric Ex/Em 535/587 nm, range 0.2 µM - 50 µM. Review our to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
L-Lactate also known as lactate is a byproduct of anaerobic glycolysis where it plays an important role in energy metabolism. L-Lactate is a small molecule with a molecular mass of approximately 90.08 g/mol. It forms in various tissues like muscle cells during intense exercise when oxygen levels are low. This process leads to a conversion of pyruvate to lactate by the action of the enzyme lactate dehydrogenase (LDH) which is present in many tissues with higher expressions in muscles and heart.
- Biological function summary
L-Lactate acts as a signaling molecule which affects cellular functions and contributes to metabolic regulation. It is not part of a complex but serves as an important intermediate in metabolic pathways. L-Lactate provides energy to cells by converting back to pyruvate in the presence of oxygen which then enters the citric acid cycle. This conversion and its utilization as energy play important roles in balancing cellular energy demands especially under hypoxic conditions.
- Pathways
L-Lactate links to critical processes like glycolysis and the Cori cycle. During glycolysis pyruvate may convert to L-Lactate under anaerobic conditions to regenerate NAD+ necessary for glycolysis to continue. In the Cori cycle lactate produced by anaerobic glycolysis in muscles is released into the bloodstream and transported to the liver. There it converts back to glucose supporting gluconeogenesis. These processes highlight the close involvement of L-Lactate with proteins such as lactate dehydrogenase and pyruvate kinase.
- Associated diseases and disorders
L-Lactate is associated with conditions like lactic acidosis and cancer. Lactic acidosis characterized by high lactate levels can occur due to oxygen deprivation or mitochondrial dysfunction. Meanwhile cancer cells often show enhanced glycolysis and lactate production known as the Warburg effect facilitating their growth. Proteins like hypoxia-inducible factor 1-alpha (HIF-1α) and lactate dehydrogenase (LDH) are key players in these conditions influencing lactate metabolism and potentially serving as therapeutic targets.
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6 product images
Image from Lai D., PLiS One 8(9), Fig 6C. Doi: 10.1371/journal.pone.0075625. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)
HaCaT cells are grown in normoxia for 3 days after infection and medium was tested to determine lactate concentration using L-Lactate assay kit (ab65330). HaCaT cells were infected with AdGFP-18E2 or AdGFP, transfected with Ctrl or HIF-1α siRNA.
Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)
Relative signal (RFU) in unfiltered human plasma (dilution 1:200) and mouse urine (dilution 1:200), comparing L-lactate signals with background readings ((-); no enzyme) after 10 minutes of incubation (duplicates +/- SD).
Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)
Lactate measured in cell culture supernatants showing quantity (μmol) per mL of tested sample. Samples were diluted 40-160 fold and measured fluorometrically.
Functional Studies - L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)
Lactate measured in biological fluids showing quantity (μmol) per mL of tested sample. Samples were diluted 10-40 fold and measured fluorometrically.
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