High Sensitivity L-Lactate Assay Kit (ab169557) is suitable for measuring very low levels of L(+)-lactate in a variety of samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Publications
Filter by
- Bioengineered 13:2554-25672022LncRNA LINC00525 activates HIF-1α through miR-338-3p / UBE2Q1 / β-catenin axis to regulate the Warburg effect in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFanqi Meng,Xiaofan Luo,Chenyao Li,Guangyi WangPubMed 35156520
- International journal of molecular sciences 22:2021Increased Beta-Hydroxybutyrate Level Is Not Sufficient for the Neuroprotective Effect of Long-Term Ketogenic Diet in an Animal Model of Early Parkinson's Disease. Exploration of Brain and Liver Energy Metabolism Markers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatarzyna Z Kuter,Łukasz Olech,Urszula Głowacka,Martyna PalecznaPubMed 34299176
- Cell reports 34:1086782021Metabolic compensation activates pro-survival mTORC1 signaling upon 3-phosphoglycerate dehydrogenase inhibition in osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRicha Rathore,Katharine E Caldwell,Charles Schutt,Caitlyn B Brashears,Bethany C Prudner,William R Ehrhardt,Cheuk Hong Leung,Heather Lin,Najat C Daw,Hannah C Beird,Abigail Giles,Wei-Lien Wang,Alexander J Lazar,John S A Chrisinger,J Andrew Livingston,Brian A Van TinePubMed 33503424
- EMBO molecular medicine 12:e124232020Dietary magnesium supplementation improves lifespan in a mouse model of progeria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRicardo Villa-BellostaPubMed 32875720
- Cancer cell international 19:2522019Circular RNA CDR1as sponges miR-7-5p to enhance E2F3 stability and promote the growth of nasopharyngeal carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiong Zhong,Juncong Huang,Jiawang Wei,Renrui WuPubMed 31582908
- Journal of neurochemistry 148:63-792018Astrocyte support is important for the compensatory potential of the nigrostriatal system neurons during early neurodegeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatarzyna Kuter,Łukasz Olech,Urszula Głowacka,Martyna PalecznaPubMed 30295916
- FEBS letters 590:2076-852016The tumor suppressor gene lkb1 is essential for glucose homeostasis during zebrafish early development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXia Kuang,Chao Liu,Junshun Fang,Weirui Ma,Jian Zhang,Sheng CuiPubMed 27264935
- PloS one 9:e1098482014A novel model of chronic wounds: importance of redox imbalance and biofilm-forming bacteria for establishment of chronicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandeep Dhall,Danh Do,Monika Garcia,Dayanjan Shanaka Wijesinghe,Angela Brandon,Jane Kim,Antonio Sanchez,Julia Lyubovitsky,Sean Gallagher,Eugene A Nothnagel,Charles E Chalfant,Rakesh P Patel,Neal Schiller,Manuela Martins-GreenPubMed 25313558
Key facts
- Detection method
Fluorescent
- Sample types
Plasma, Tissue, Suspension cells, Serum, Other biological fluids, Adherent cells
- Assay type
Quantitative
- Reactive species
Mammals
- Range
1000 - 250000 nmol/well
- Sensitivity
< 0.2 µM
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
What's included?
Recommended products
High Sensitivity L-Lactate Assay Kit (ab169557) is suitable for measuring very low levels of L(+)-lactate in a variety of samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Fluorescent
- Sample types
Plasma, Tissue, Suspension cells, Serum, Other biological fluids, Adherent cells
- Assay type
Quantitative
- Reactive species
Mammals
- Range
1000 - 250000 nmol/well
- Assay Platform
Microplate reader
- Sensitivity
< 0.2 µM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
High Sensitivity L-Lactate Assay Kit (ab169557) is suitable for measuring very low levels of L(+)-lactate in a variety of samples.
In this assay, L(+)-lactate is specifically oxidized to form an intermediate that reacts with a colorless probe to generate fluorescence (Ex/Em = 535/587 nm), which is directly proportional to the amount of lactate.
This simple, rapid and high-throughput suitable assay kit is the most sensitive lactate assay kit on the market. It can detect L(+)-lactate less than 0.2 μM in a variety of biological samples.
L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present (see ) but only at about 1-5% of the concentration of L(+)-Lactate. Alternative L-Lactate assay kits offer different readout modes/wavelengths and sensitivity/range: - this kit L-Lactate assay ab169557: fluorometric Ex/Em 535/587 nm, range 0.2 µM - 50 µM - our most popular : colorimetric 450nm, range 0.02 mM - 10 mM - : colorimetric 570 nm, fluorometric Ex/Em 535/587 nm, range 0.001 mM - 10 mM Review our to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
L-Lactate also known as lactate is a byproduct of anaerobic glycolysis where it plays an important role in energy metabolism. L-Lactate is a small molecule with a molecular mass of approximately 90.08 g/mol. It forms in various tissues like muscle cells during intense exercise when oxygen levels are low. This process leads to a conversion of pyruvate to lactate by the action of the enzyme lactate dehydrogenase (LDH) which is present in many tissues with higher expressions in muscles and heart.
- Biological function summary
L-Lactate acts as a signaling molecule which affects cellular functions and contributes to metabolic regulation. It is not part of a complex but serves as an important intermediate in metabolic pathways. L-Lactate provides energy to cells by converting back to pyruvate in the presence of oxygen which then enters the citric acid cycle. This conversion and its utilization as energy play important roles in balancing cellular energy demands especially under hypoxic conditions.
- Pathways
L-Lactate links to critical processes like glycolysis and the Cori cycle. During glycolysis pyruvate may convert to L-Lactate under anaerobic conditions to regenerate NAD+ necessary for glycolysis to continue. In the Cori cycle lactate produced by anaerobic glycolysis in muscles is released into the bloodstream and transported to the liver. There it converts back to glucose supporting gluconeogenesis. These processes highlight the close involvement of L-Lactate with proteins such as lactate dehydrogenase and pyruvate kinase.
- Associated diseases and disorders
L-Lactate is associated with conditions like lactic acidosis and cancer. Lactic acidosis characterized by high lactate levels can occur due to oxygen deprivation or mitochondrial dysfunction. Meanwhile cancer cells often show enhanced glycolysis and lactate production known as the Warburg effect facilitating their growth. Proteins like hypoxia-inducible factor 1-alpha (HIF-1α) and lactate dehydrogenase (LDH) are key players in these conditions influencing lactate metabolism and potentially serving as therapeutic targets.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Functional Studies - L-Lactate Assay Kit (Fluorometric, High Sensitivity) (ab169557)
Relative signal (RFU) in filtered mouse heart lysate (3.7 mg protein/mL prior to filtration) in different volumes, comparing L-Lactate signals (black) with background reading (white, no enzyme).
Functional Studies - L-Lactate Assay Kit (Fluorometric, High Sensitivity) (ab169557)
L-Lactate measured in unfiltered human plasma (dilution range 1:30-1:90) and mouse serum (dilution range 1:2500-1:7500); duplicates +/- SD. The background control levels (no enzyme) were below level of detection.
Functional Studies - L-Lactate Assay Kit (Fluorometric, High Sensitivity) (ab169557)
Lactate Levels in Various Samples. Measurement of Lactate levels in rat liver (1.2 µg), kidney (0.7 µg), muscle (0.45 µg) and in human serum (0.5 µL from 1:10 diluted serum). Assays were performed following kit protocol.
Functional Studies - L-Lactate Assay Kit (Fluorometric, High Sensitivity) (ab169557)
Standard curve with background signal subtracted (duplicates; +/- SD).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com