Laccase Activity Assay Kit ab284539 is a simple, one step, plate based assay to measure Laccase activity in various samples.
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Key facts
- Sample types
Cell Lysate, Purified protein
- Sensitivity
= 20 µU
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Enzyme activity assay | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Purine nucleoside enzyme that catalyzes the phosphorolysis of adenosine, guanosine and inosine nucleosides, yielding D-ribose 1-phosphate and the respective free bases, adenine, guanine and hypoxanthine (PubMed:31978345). Also catalyzes the phosphorolysis of S-methyl-5'-thioadenosine into adenine and S-methyl-5-thio-alpha-D-ribose 1-phosphate (PubMed:31978345). Also has adenosine deaminase activity (PubMed:31978345). Acts as a regulator of innate immunity in macrophages by modulating the purine nucleotide metabolism, thereby regulating the metabolic function and bioenergetic state of macrophages (PubMed:31978345). Enables a purine nucleotide cycle between adenosine and inosine monophosphate and adenylosuccinate that prevents cytoplasmic acidification and balances the cytoplasmic-mitochondrial redox interface (PubMed:31978345). The purine nucleotide cycle consumes aspartate and releases fumarate in a manner involving fatty acid oxidation and ATP-citrate lyase activity (PubMed:31978345). Participates in pattern recognition receptor (PRR)-induced cytokines in macrophages: associates with the NOD2-signaling complex and promotes optimal NOD2-induced signaling, cytokine secretion and bacterial clearance (PubMed:28593945, PubMed:31875558). Localizes to the endoplasmic reticulum upon PRR stimulation of macrophages and associates with endoplasmic reticulum-stress sensors, promoting the endoplasmic reticulum unfolded protein response (UPR) (PubMed:31875558). Does not show laccase activity (PubMed:27959965, PubMed:31978345).
Alternative names
Purine nucleoside phosphorylase LACC1, Adenosine deaminase LACC1, Fatty acid metabolism-immunity nexus, Guanosine phosphorylase LACC1, Laccase domain-containing protein 1, S-methyl-5'-thioadenosine phosphorylase LACC1, LACC1, C13orf31, FAMIN
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Laccase Activity Assay Kit ab284539 is a simple, one step, plate based assay to measure Laccase activity in various samples.
Alternative names
Purine nucleoside phosphorylase LACC1, Adenosine deaminase LACC1, Fatty acid metabolism-immunity nexus, Guanosine phosphorylase LACC1, Laccase domain-containing protein 1, S-methyl-5'-thioadenosine phosphorylase LACC1, LACC1, C13orf31, FAMIN
Key facts
- Sample types
Cell Lysate, Purified protein
- Assay Platform
Microplate
- Sensitivity
= 20 µU
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
This Laccase Activity Assay Kit (ab284539) (K2038) is a simple, one step, plate based assay to measure Laccase activity in various samples. In this assay, samples containing Laccase will oxidize the substrate to generate a colored product with a strong absorbance at 420 nm.
The kit is rapid, easy to use and high-throughput adaptable. It can measure Laccase activity as low as 20 μU in samples.
This product is manufactured by BioVision, an Abcam company and was previously called K2038 Laccase Activity Assay Kit (Colorimetric). K2038-100 is the same size as the 100 test size of ab284539.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Laccase also known as EC 1.10.3.2 is an enzyme that facilitates the oxidation of phenolic and non-phenolic compounds by reducing molecular oxygen to water. It belongs to the multi-copper oxidase family and has an approximate mass of 60-80 kDa. Expression of laccase is commonly found in plants fungi and bacteria. Its enzymatic action involves the transfer of electrons important for the degradation of various substrates often observed using the 'laccase activity assay'.
- Biological function summary
Laccase participates in lignin degradation and polymerization which makes it important in carbon cycling and biodegradation processes. It does not specifically form part of a known protein complex but acts as an individual catalyst facilitating the breakdown of complex organic molecules. Researchers often use 'laccase assay' methods to study its activity in these biological processes. This activity is essential for the breakdown of plant materials affecting the nutrient availability in ecosystems.
- Pathways
Laccase operates in the phenylpropanoid and lignin biosynthesis pathways. These pathways are important for the synthesis and breakdown of lignin in plants among other functions. Laccase is related to proteins such as peroxidase involved in similar oxidative reactions. 'Laccase assay protocol' provides insight into its role and interaction within these biochemical routes which is significant for understanding plant resistance and adaptation mechanisms.
- Associated diseases and disorders
Laccase plays a role in pathological conditions like asthma and neurodegenerative diseases such as Alzheimer's. In asthma its activity is linked to inflammation where it may influence the regulation of oxidative stress. Laccase can interact with other proteins linked to oxidative stress pathways affecting how cells respond to stress and damage. Understanding laccase's function and its potential therapeutic applications may guide research in controlling symptoms and progression of these diseases.
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2 product images
Functional Studies - Laccase Activity Assay Kit (Colorimetric) (ab284539)
Laccase Activity Assay Kit (Colorimetric).
Laccase specific activity in potato and mushroom lysate. Experiments were conducted according to kit protocol.
Functional Studies - Laccase Activity Assay Kit (Colorimetric) (ab284539)
Laccase Activity Assay Kit (Colorimetric).
Enzyme kinetics using mushroom lysate (5 μg protein per well) and potato lysate (0.62 μg protein per well). Experiments were conducted according to kit protocol.
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