Lactate Dehydrogenase B / LDH-B Activity Assay

• FuncS

Supplier Data

Functional Studies - Lactate Dehydrogenase B / LDH-B Activity Assay (AB140361)

Species cross reactivity shown with sera. The species cross reactivity of this assay was demonstrated by testing the sera from multiple species. The kit shows a good reaction with human, goat and bovine samples, but shows no reaction with mouse or rat sera which may also contain LDHB. This species cross reaction specificity is due to the species specificity of the capture antibody.

• FuncS

Supplier Data

Functional Studies - Lactate Dehydrogenase B / LDH-B Activity Assay (AB140361)

Example control standard curve generaated using the method described in the protocl booklet.

• FuncS

Supplier Data

Functional Studies - Lactate Dehydrogenase B / LDH-B Activity Assay (AB140361)

Lysate example curve. LDHB activity was measured from Hela cell lysate, which contains hetero-tetramer isozyme composed of both (M) LDHA and (H) LDHB subunits. However, the liver derived cell line, HepG2, which contains mainly homo-tetramer of subunit M (LDHA), shows no enzymatic activity.

• WB

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Western blot - Lactate Dehydrogenase B / LDH-B Activity Assay (AB140361)

Isoform specificity validated by Western blot.

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : LDHA (purified from rabbit skeletal muscle)
Lane 4 : LDHB (recombinant)
Lane 5 : HeLa – In-well extracted with capture mAb
Lane 6 : HepG2 – In-well extracted with capture mAb
Lane 7 : LDHA – In-well extracted with a capture mAb
Lane 8 : LDHB – In-well extracted with capture mAb

The western blot results indicate that the capture antibody isolates active LDHB, but does not isolate LDHA (which would be detected by the anti-LDH antibody).

false