AB197000

Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric)

Name: Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000) | Abcam
Brand: Abcam Limited
SKU: AB197000
Price: 670 USD
Availability: InStock

Be the first to review this product! Submit a review

(17 Publications)

Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000) provides a quick and easy method for monitoring Lactate Dehydrogenase (LDH) activity in a wide variety of samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

3 Images

Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (AB197000)

FuncS

Supplier Data

Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (AB197000)

Kinetic measurement of Lactate Dehydrogenase activity in a range of biological samples.

FuncS

Supplier Data

Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (AB197000)

Typical NADH Standard Curve obtained following assay protocol.

FuncS

Supplier Data

Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (AB197000)

Relative LDH Activity was calculated in lysates prepared from rat liver (0.037 μg protein), Jurkat cells (0.053 μg protein), and Human serum (0.2 μg protein).

Key facts

Detection method

Fluorescent

Sample types

Urine, Plasma, Suspension cells, Tissue Extracts, Serum, Other biological fluids, Adherent cells, Cell culture media (without Phenol Red)

Assay type

Enzyme activity

Sensitivity

= 1 µU/mL

Assay time

20m

Assay Platform

Microplate reader

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000) provides a quick and easy method for monitoring Lactate Dehydrogenase (LDH) activity in a wide variety of samples. In this assay, LDH converts lactate into pyruvate and NADH, which reacts with the specific fluorescent probe to generate an intense fluorescent product (Ex/Em = 535/587 nm).

This kit is simple, highly sensitive and high-throughput adaptable and can detect LDH activity as low as 1 uU/mL.

LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader every 2-3 min for 10-30 min

Other Notes
This product was previously called K730 PicoProbe Lactate Dehydrogenase Activity Assay Kit. Biovision was acquired by Abcam in 2021.

Lactate dehydrogenase (LDH, L-Lactate NAD oxidoreductase, EC 1.1.1.27) is an ubiquitous enzymes among vertebrate organisms which catalyzes the reversible conversion of pyruvate to lactate, with concomitant conversion of NADH and NAD+. LDH is cytoplasmic in its cellular location and in any one tissue is composed of one or two of five possible isoenzymes. During tissue damage, LDH is released into the bloodstream; therefore it serves as a marker for various diseases and common injuries.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style

left-column

What's included?

{ "values": { "500Test": { "sellingSize": "500 Test", "publicAssetCode":"ab197000-500Test", "assetComponentDetails": [ { "size":"1 x 1.4 mL", "name":"PicoProbe", "number":"AB197000-CMP04", "productcode":"" }, { "size":"1 x 110 mL", "name":"LDH Assay Buffer", "number":"AB197000-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"LDH Positive Control", "number":"AB197000-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix H", "number":"AB197000-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NADH Standard I", "number":"AB197000-CMP05", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lactate dehydrogenase (LDH) is an enzyme that catalyzes the interconversion of pyruvate and lactate along with the conversion of NADH to NAD+. LDH is known by other names such as lactic acid dehydrogenase and LDH-5. The enzyme has a molecular weight of approximately 36 kDa. LDH exists in almost all tissues having multiple isoforms that are expressed differently depending on the tissue type. It shows high expression in muscle tissue liver and heart indicating its extensive role in energy metabolism.

Biological function summary

Lactate dehydrogenase plays a critical role in anaerobic glycolysis. The enzyme helps in regenerating NAD+ from NADH allowing glycolysis to continue in the absence of oxygen. LDH is not a part of any larger protein complex working independently to fulfill its function in the glycolytic pathway. It serves in rapid energy production especially under hypoxic or exertional conditions where oxygen supply is limited.

Pathways

LDH is significantly involved in the glycolysis and gluconeogenesis pathways. Within glycolysis LDH helps facilitate the conversion of pyruvate to lactate during anaerobic conditions a step important for ATP production when oxygen is scarce. The enzyme is tied closely to phosphofructokinase-1 (PFK-1) in glycolysis given that both enzymes are central to maintaining the glycolytic flow. In gluconeogenesis though functionally reversed from its role in glycolysis LDH helps to manage lactate removal an important step for glucose synthesis from non-carbohydrate sources.

Lactate dehydrogenase levels often act as a biomarker for tissue damage or certain cancers as its release into the bloodstream signals cellular injury or death. Elevated LDH levels are associated with conditions like myocardial infarction and certain forms of anemia. In cancer such as lymphoma or leukemia LDH correlates with the progression of the disease and acts as a prognostic marker. LDH's connection to these conditions often leads to insights into disease severity and progression due to its association with proteins like p53 and HIF-1 which play roles in cellular metabolism and hypoxia response.

style

left-column

Product protocols

style

left-column

Target data

See full target information LDH

style

left-column

Publications (17)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed38139026

2023

Adipose-Derived Mesenchymal Stem Cells Protect Endothelial Cells from Hypoxic Injury by Suppressing Terminal UPR In Vivo and In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Keese,Jiaxing Zheng,Kaixuan Yan,Karen Bieback,Benito A Yard,Prama Pallavi,Christoph Reissfelder,Mark Andreas Kluth,Martin Sigl,Vugar Yugublu

Polymers 15: PubMed37960012

2023

A Dexamethasone-Loaded Polymeric Electrospun Construct as a Tubular Cardiovascular Implant.

Applications

Unspecified application

Species

Unspecified reactive species

Stavroula Kyriakou,Sergio Acosta,Ikram El Maachi,Stephan Rütten,Stefan Jockenhoevel

Stem cell research & therapy 14:24 PubMed36759868

2023

ABCB5 mesenchymal stromal cells therapy protects from hypoxia by restoring Ca homeostasis in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Kaixuan Yan,Jiaxing Zheng,Mark Andreas Kluth,Lin Li,Christoph Ganss,Benito Yard,Richard Magdeburg,Markus H Frank,Prama Pallavi,Michael Keese

Journal of controlled release : official journal of the Controlled Release Society 349:796-811 PubMed35914613

2022

CD44 receptor targeted nanoparticles augment immunity against tuberculosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Vipul K Singh,Eric Chau,Abhishek Mishra,Alexandro DeAnda,Venkatesh L Hegde,Jagannadha K Sastry,David Haviland,Chinnaswamy Jagannath,Biana Godin,Arshad Khan

Journal of neurochemistry 162:226-244 PubMed35304760

2022

Copper bis(thiosemicarbazone) complexes modulate P-glycoprotein expression and function in human brain microvascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jae Pyun,Lachlan E McInnes,Paul S Donnelly,Celeste Mawal,Ashley I Bush,Jennifer L Short,Joseph A Nicolazzo

Biomedicines 9: PubMed34572378

2021

Self-Organization Provides Cell Fate Commitment in MSC Sheet Condensed Areas via ROCK-Dependent Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Peter Nimiritsky,Ekaterina Novoseletskaya,Roman Eremichev,Natalia Alexandrushkina,Maxim Karagyaur,Oleg Vetrovoy,Nataliya Basalova,Anastasia Khrustaleva,Alexander Tyakht,Anastasia Efimenko,Vsevolod Tkachuk,Pavel Makarevich

Cell biochemistry and function 39:802-812 PubMed34129243

2021

Distinct glycolytic pathway regulation in liver, tumour and skeletal muscle of mice with cancer cachexia.

Applications

Unspecified application

Species

Unspecified reactive species

Nishant P Visavadiya,Harry B Rossiter,Andy V Khamoui

The Journal of international medical research 49:300060521999558 PubMed33752450

2021

Clinical features of cardiac lymphoma: an analysis of 37 cases.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhao,Sai Huang,Chao Ma,Haiyan Zhu,Jian Bo

Turkish journal of pharmaceutical sciences 17:506-510 PubMed33177931

2020

Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Çiğdem Sevİm,Ali Taghİzadehghalehjoughİ,Mehtap Kara

American journal of translational research 12:3412-3428 PubMed32774709

2020

Inner mitochondrial membrane protein MPV17 mutant mice display increased myocardial injury after ischemia/reperfusion.

Applications

Unspecified application

Species

Mouse

Ngonidzashe B Madungwe,Yansheng Feng,Abdulhafiz Imam Aliagan,Nathalie Tombo,Ferdinand Kaya,Jean C Bopassa

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric)

Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (AB197000)

Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (AB197000)

Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (AB197000)

Key facts

Detection method

Sample types

Assay type

Sensitivity

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

Product promise