Skip to main content

This product is a bundle of 2 x BOND Titration Containers (with 6mL inserts) along with 100 uL vial of Anti-Calreticulin antibody [EPR3924] - ER Marker - ab92516.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LBS RX - Anti-Calreticulin antibody [EPR3924] - ER Marker (AB321951), expandable thumbnail

Reactivity data

Application

IHC-P

Reactivity

Reacts

Dilution info
0.01 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed:7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed:11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).

Alternative names

CRTC, CALR, Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, HACBP, grp60, ERp60

What's included?

1 Kit
Components
Anti-Calreticulin antibody [EPR3924] - ER Marker
1 x 100 µL
LBS Rx dispenser
2 x 1 Unit

This product is a bundle of 2 x BOND Titration Containers (with 6mL inserts) along with 100 uL vial of Anti-Calreticulin antibody [EPR3924] - ER Marker - ab92516.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

This product is a bundle of 2 x BOND Titration Containers (with 6mL inserts) along with 100 uL vial of Anti-Calreticulin antibody [EPR3924] - ER Marker - Anti-Calreticulin antibody [EPR3924] - ER Marker ab92516.


Additional Titration Inserts can be purchased from Leica Biosystems, using OPT9719 (box of 50). Or Titration Container/Insert Kit, using OPT9049 (box of 10/50).

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LBS RX - Anti-Calreticulin antibody [EPR3924] - ER Marker (ab321951), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LBS RX - Anti-Calreticulin antibody [EPR3924] - ER Marker (ab321951)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labelling Calreticulin with Anti-Calreticulin antibody [EPR3924] - ER Marker ab92516 [EPR3924] at 0.01 µg/mL concentration.

    All steps were performed at room temperature unless otherwise stated.

    Staining was performed on the BOND RX Advanced Staining Instrument from Leica Biosystems, using the Bond Polymer Refine Detection kit (DS9800) and the following protocols:

    1. Deparaffinization - Dewax
    2. Antigen retrieval - HIER with ER2 (pH 9.0), 20 minutes, 100°C
    3. Staining – modified IHC Protocol F (Marker: Anti-Calreticulin antibody [EPR3924] - ER Marker ab92516 for 15 minutes with 3 x Bond Wash Solution steps) where standard post-primary step has been excluded.

    Sections were counterstained with Hematoxylin (DS9800).

    Secondary Antibody only control was performed with the same staining protocols, with marker as negative.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com