AB65391

LDH Assay Kit (Cytotoxicity)

Name: LDH Assay Kit (Cytotoxicity) (ab65391/K311-400) | Abcam
Brand: Abcam Limited
SKU: AB65391
Price: 445 USD
Availability: InStock

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(37 Publications)

LDH Assay Kit ab65391 provides a fast and simple method cytotoxicity assay based on measurement of activity of LDH released from damaged cells.For a more sensitive LDH Assay Kit to use as a cytotoxicity assay, we recommend ab65393.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

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Functional Studies - LDH Assay Kit (Cytotoxicity) (AB65391)

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Lab

Functional Studies - LDH Assay Kit (Cytotoxicity) (AB65391)

LDH release measured at various cell amounts

FuncS

Supplier Data

Functional Studies - LDH Assay Kit (Cytotoxicity) (AB65391)

Jurkat cells were cultured in a 96-well plate in 100 µl of culture medium. LDH assay was performed using 10 µl of culture medium according to the kit instructions. Light bar : Low control; Dark bar : High control.

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Enzyme activity

Assay time

Assay Platform

Microplate reader

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Product details

For a more sensitive LDH Assay Kit to use as a cytotoxicity assay, we recommend ab65393.

LDH Assay Kit (Cytotoxicity) ab65391 provides a fast and simple method for quantitating cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells.

The assay takes ~0.5-1 hr. LDH activity can be determined by a coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which then reacts with tetrazolium salt INT to form formazan. The increase in the amount of formazan produced in culture supernatant directly correlates to the increase in the number of lysed cells. The formazan dye is water-soluble and can be detected by spectrophotometer at 500 nm.

This kit was previously called LDH-Cytotoxicity Assay Kit I.

Other Notes
Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Unlike many other cytoplasmic enzymes which exist in many cells either in low amount (e.g., alkaline and acid phosphatase) or unstable, LDH is a stable cytoplasmic enzyme present in all cells and rapidly released into the cell culture supernatant upon damage of the plasma membrane.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lactate dehydrogenase (LDH) is an enzyme that catalyzes the interconversion of pyruvate and lactate along with the conversion of NADH to NAD+. LDH is known by other names such as lactic acid dehydrogenase and LDH-5. The enzyme has a molecular weight of approximately 36 kDa. LDH exists in almost all tissues having multiple isoforms that are expressed differently depending on the tissue type. It shows high expression in muscle tissue liver and heart indicating its extensive role in energy metabolism.

Biological function summary

Lactate dehydrogenase plays a critical role in anaerobic glycolysis. The enzyme helps in regenerating NAD+ from NADH allowing glycolysis to continue in the absence of oxygen. LDH is not a part of any larger protein complex working independently to fulfill its function in the glycolytic pathway. It serves in rapid energy production especially under hypoxic or exertional conditions where oxygen supply is limited.

Pathways

LDH is significantly involved in the glycolysis and gluconeogenesis pathways. Within glycolysis LDH helps facilitate the conversion of pyruvate to lactate during anaerobic conditions a step important for ATP production when oxygen is scarce. The enzyme is tied closely to phosphofructokinase-1 (PFK-1) in glycolysis given that both enzymes are central to maintaining the glycolytic flow. In gluconeogenesis though functionally reversed from its role in glycolysis LDH helps to manage lactate removal an important step for glucose synthesis from non-carbohydrate sources.

Lactate dehydrogenase levels often act as a biomarker for tissue damage or certain cancers as its release into the bloodstream signals cellular injury or death. Elevated LDH levels are associated with conditions like myocardial infarction and certain forms of anemia. In cancer such as lymphoma or leukemia LDH correlates with the progression of the disease and acts as a prognostic marker. LDH's connection to these conditions often leads to insights into disease severity and progression due to its association with proteins like p53 and HIF-1 which play roles in cellular metabolism and hypoxia response.

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Product protocols

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Target data

See full target information LDH

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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Drug delivery and translational research 15:2814-2832 PubMed39779651

2025

When conventional approach in toxicity assays falls short for nanomedicines: a case study with nanoemulsions.

Applications

Unspecified application

Species

Unspecified reactive species

Ines Nikolić,Jelena Đoković,Dora Mehn,Giuditta Guerrini,Snežana Savić,Olivier Jordan,Gerrit Borchard

Molecular neurobiology 59:7423-7438 PubMed36190692

2022

Transcranial Direct-Current Stimulation Regulates MCT1-PPA-PTEN-LONP1 Signaling to Confer Neuroprotection After Rat Cerebral Ischemia-Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyi Kong,Wenjie Hu,Yu Cui,Jingchen Gao,Xujin Yao,Jinyang Ren,Tao Lin,Jiangdong Sun,Yunyi Gao,Xiaohua Li,Hui Wang,Huanting Li,Fengyuan Che,Qi Wan

International journal of pharmaceutics: X 4:100124 PubMed35898812

2022

Identification of novel cyanoacrylate monomers for use in nanoparticle drug delivery systems prepared by miniemulsion polymerisation - A multistep screening approach.

Applications

Unspecified application

Species

Unspecified reactive species

Astrid Hyldbakk,Yrr Mørch,Sofie Snipstad,Andreas K O Åslund,Geir Klinkenberg,Vu To Nakstad,Ane-Marit Wågbø,Ruth Schmid,Peter P Molesworth

Nanomaterials (Basel, Switzerland) 12: PubMed35745286

2022

In Vitro High-Throughput Toxicological Assessment of Nanoplastics.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Tolardo,Davide Magrì,Francesco Fumagalli,Domenico Cassano,Athanassia Athanassiou,Despina Fragouli,Sabrina Gioria

JCI insight 7: PubMed35420998

2022

Kisspeptins inhibit human airway smooth muscle proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Niyati A Borkar,Nilesh Sudhakar Ambhore,Rama Satyanarayana Raju Kalidhindi,Christina M Pabelick,Y S Prakash,Venkatachalem Sathish

Frontiers in oncology 12:693395 PubMed35321425

2022

Inhibits Hepatocellular Carcinoma Tumorigenicity by Inducing Ferroptosis of Hepatocellular Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Li,Jiongshan Zhang,Kun Zhang,Yan Chen,Wei Wang,Hongjie Chen,Zengcheng Zou,Yongwei Li,Min Dai

IUBMB life 74:927-942 PubMed35218610

2022

The effects of sulfated hyaluronan in breast, lung and colorectal carcinoma and monocytes/macrophages cells: Its role in angiogenesis and tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Fiorella M Spinelli,Paolo Rosales,Stefano Pluda,Daiana L Vitale,Antonella Icardi,Cristian Guarise,Andrea Reszegi,Ilona Kovalszky,Mariana García,Ina Sevic,Devis Galesso,Laura Alaniz

PLoS pathogens 17:e1010018 PubMed34695168

2021

Interaction between coxsackievirus B3 infection and α-synuclein in models of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Soo Jin Park,Uram Jin,Sang Myun Park

Bioengineered 12:5361-5372 PubMed34427537

2021

The protective effects of naproxen against interleukin-1β (IL-1β)- induced damage in human umbilical vein endothelial cells (HUVECs).

Applications

Unspecified application

Species

Unspecified reactive species

Yuliang Wu,Ruina Hao,Beidi Lan,Yiping Mu,Fuping Dang,Ruitao Wang

Bioengineered 12:5361-5372 PubMed34427537

2021

The protective effects of naproxen against interleukin-1β (IL-1β)- induced damage in human umbilical vein endothelial cells (HUVECs).

Applications

Unspecified application

Species

Unspecified reactive species

Yuliang Wu,Ruina Hao,Beidi Lan,Yiping Mu,Fuping Dang,Ruitao Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

LDH Assay Kit (Cytotoxicity)

Functional Studies - LDH Assay Kit (Cytotoxicity) (AB65391)

Functional Studies - LDH Assay Kit (Cytotoxicity) (AB65391)

Key facts

Sample types

Assay type

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (37)

Product promise