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Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, tissue, cells, and culture medium.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.


Images

Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (AB102526), expandable thumbnail
  • Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (AB102526), expandable thumbnail
  • Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (AB102526), expandable thumbnail
  • Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (AB102526), expandable thumbnail

Publications

  • Biomolecules & therapeutics 32:349-3602024
    Morroniside Protects C2C12 Myoblasts from Oxidative Damage Caused by ROS-Mediated Mitochondrial Damage and Induction of Endoplasmic Reticulum Stress.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Hyun Hwangbo,Cheol Park,EunJin Bang,Hyuk Soon Kim,Sung-Jin Bae,Eunjeong Kim,Youngmi Jung,Sun-Hee Leem,Young Rok Seo,Su Hyun Hong,Gi-Young Kim,Jin Won Hyun,Yung Hyun Choi
    PubMed 38602043
  • Allergologia et immunopathologia 52:16-232024
    Polyphyllin II inhibits NLPR3 inflammasome activation and inflammatory response of -infected human bronchial epithelial cells.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Guodong Cheng,Gengzhi Ye,Ying Ma,Yuqing Wang
    PubMed 38186190

Key facts

Detection method

Colorimetric

Sample types

Plasma, Urine (UTI), Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Assay type

Enzyme activity (quantitative)

Range

1 - 100 mU/mL

Assay time

30m

Sensitivity

> 1 mU/mL

Associated Products

Select an associated product type

2 products for Alternative Product

Alternative names

What's included?

500 Test
Components
LDH Assay Buffer
1 x 50 mL
LDH Positive Control
1 x 1 Vial
NADH Standard I
1 x 1 Vial
Substrate Mix
1 x 1 Vial

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Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, tissue, cells, and culture medium.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Plasma, Urine (UTI), Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Assay type

Enzyme activity (quantitative)

Range

1 - 100 mU/mL

Assay time

30m

Assay Platform

Microplate reader

Sensitivity

> 1 mU/mL

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, tissue, cells, and culture medium.

In the LDH assay protocol, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (OD max = 450 nm). The kit can detect 1 - 100 mU/mL of LDH directly in samples. The assay is quick, convenient, and sensitive.

LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze every 2-3 min for at least 30 min with microplate reader in kinetic mode at 37°C

Technical Note: LDH activity is unlikely to be detectable in healthy urine but may be elevated in the presence of infection or disease of the urinary tract.

This product is manufactured by BioVision, an Abcam company and was previously called K726 Lactate Dehydrogenase Activity Colorimetric Assay Kit. K726-500 is the same size as the 500 test size of ab102526.

To measure LDH release into cell culture medium from cultured cells in a cytoxicity experiment, we recommend LDH assay kit LDH Assay Kit (Cytotoxicity) ab65393, which is designed specifically as a cytotoxicity assay.

This LDH assay kit ab102526 is designed in a more flexible format for use with a variety of sample types (this includes cell culture medium, and this kit may be used in cytotoxicity assays). This flexible format LDH assay kit is also available as a fluorometric LDH assay kit ab197000.

How other researchers have used LDH Assay Kit ab102526

This LDH assay kit has been used in publications in a variety of sample types, including:
- Human: serum1
- Mouse: kidney tissue2, kidney epithelial cell line lysates3, muscle tissue4, serum2,5, vaginal lavage fluid6, liver tissue7
- Rat: H8C2 cell lysates8

It has also been used in LDH release cytotoxicity cell culture assays including in human hippocampal neuronal cultures9, THP-1 cells10, primary chondrocyte cells on a membrane scaffold11, primary lymphocytes12, ovarian cancer cells co-cultured with mouse splenocytes in a cytotoxic T lymphocyte assay13

References: 1-Zhang Z et al 2018, Solivio MJ et al 2016; 2-Niles DJ et al 2018; 3-Tanner LB et al 2018; 4-Tam BT et al 2018; 5-Kim HY et al 2016; 6-Nash EE et al 2016; 7-Lai C et al 2018; 8-Zhou Z et al 2018; 9-Wang Q et al 2019; 10-Alimov I et al 2019; 11-Stocco TD et al 2019; 12-Dreisig K et al 2018; 13-Sun Y et al 2018

Supplementary info

Activity summary

Lactate dehydrogenase (LDH) is an enzyme that catalyzes the interconversion of pyruvate and lactate along with the conversion of NADH to NAD+. LDH is known by other names such as lactic acid dehydrogenase and LDH-5. The enzyme has a molecular weight of approximately 36 kDa. LDH exists in almost all tissues having multiple isoforms that are expressed differently depending on the tissue type. It shows high expression in muscle tissue liver and heart indicating its extensive role in energy metabolism.

Biological function summary

Lactate dehydrogenase plays a critical role in anaerobic glycolysis. The enzyme helps in regenerating NAD+ from NADH allowing glycolysis to continue in the absence of oxygen. LDH is not a part of any larger protein complex working independently to fulfill its function in the glycolytic pathway. It serves in rapid energy production especially under hypoxic or exertional conditions where oxygen supply is limited.

Pathways

LDH is significantly involved in the glycolysis and gluconeogenesis pathways. Within glycolysis LDH helps facilitate the conversion of pyruvate to lactate during anaerobic conditions a step important for ATP production when oxygen is scarce. The enzyme is tied closely to phosphofructokinase-1 (PFK-1) in glycolysis given that both enzymes are central to maintaining the glycolytic flow. In gluconeogenesis though functionally reversed from its role in glycolysis LDH helps to manage lactate removal an important step for glucose synthesis from non-carbohydrate sources.

Associated diseases and disorders

Lactate dehydrogenase levels often act as a biomarker for tissue damage or certain cancers as its release into the bloodstream signals cellular injury or death. Elevated LDH levels are associated with conditions like myocardial infarction and certain forms of anemia. In cancer such as lymphoma or leukemia LDH correlates with the progression of the disease and acts as a prognostic marker. LDH's connection to these conditions often leads to insights into disease severity and progression due to its association with proteins like p53 and HIF-1 which play roles in cellular metabolism and hypoxia response.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526), expandable thumbnail
    Tam BT et al. 2018 Sci Rep 8:3689 Use licensed under: http://creativecommons.org/licenses/by/4.0/

    Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)

    LDH assay on mouse skeletal muscle.

    Tam BT et al. used the LDH assay kit on mouse skeletal muscle tissue lysates comparing pressure-induced compressed and control samples across a range of knockout mice lines.

  • Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526), expandable thumbnail

    Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)

    Kinetic profiles of approx 0.5 mU of a sample of pure LDH (Positive Control) and 2 μL frozen human serum using buffer as a background control.

  • Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526), expandable thumbnail

    Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)

    NADH Standard Curve.

  • Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526), expandable thumbnail
    Image from Xu Y et al., PLoS One. 2014;9(6):e100127. Fig 2(B).; doi: 10.1371/journal.pone.0100127. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)

    Cellular necrosis was measured as LDH release after cisplatin treatment for 24 h and 48 h using ab102526. The release of LDH was apparently increased at 24 and 48 h after cisplatin treatment.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com