Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 is a quantitative, addition-only assay with a single 30-min incubation step. In the assay, LDH reduces NAD, followed by interaction with a probe. Readout on any colorimetric (450 nm) plate reader.
- Cited in over 240 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Biomolecules & therapeutics 32:349-3602024Morroniside Protects C2C12 Myoblasts from Oxidative Damage Caused by ROS-Mediated Mitochondrial Damage and Induction of Endoplasmic Reticulum Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun Hwangbo,Cheol Park,EunJin Bang,Hyuk Soon Kim,Sung-Jin Bae,Eunjeong Kim,Youngmi Jung,Sun-Hee Leem,Young Rok Seo,Su Hyun Hong,Gi-Young Kim,Jin Won Hyun,Yung Hyun ChoiPubMed 38602043
- Allergologia et immunopathologia 52:16-232024Polyphyllin II inhibits NLPR3 inflammasome activation and inflammatory response of -infected human bronchial epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuodong Cheng,Gengzhi Ye,Ying Ma,Yuqing WangPubMed 38186190
- World journal of stem cells 15:1093-11032024Human mesenchymal stem cells exhibit altered mitochondrial dynamics and poor survival in high glucose microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEjlal Abu-El-Rub,Fatimah Almahasneh,Ramada R Khasawneh,Ayman Alzu'bi,Doaa Ghorab,Rawan Almazari,Huthaifa Magableh,Ahmad Sanajleh,Haitham Shlool,Mohammad Mazari,Noor S Bader,Joud Al-MomaniPubMed 38179215
- Pharmaceuticals (Basel, Switzerland) 16:2023Glibenclamide-Loaded Engineered Nanovectors (GNVs) Modulate Autophagy and NLRP3-Inflammasome Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarina Saresella,Chiara Paola Zoia,Francesca La Rosa,Chiara Bazzini,Gessica Sala,Erica Grassenis,Ivana Marventano,Ambra Hernis,Federica Piancone,Elisa Conti,Silvia Sesana,Francesca Re,Pierfausto Seneci,Carlo Ferrarese,Mario ClericiPubMed 38139851
- Current issues in molecular biology 45:9926-99422023Lumbricus Extract Prevents LPS-Induced Inflammatory Activation of BV2 Microglia and Glutamate-Induced Hippocampal HT22 Cell Death by Suppressing MAPK/NF-κB/NLRP3 Signaling and Oxidative Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYou-Chang Oh,Yun Hee Jeong,Hye Jin Yang,Wei Li,Jin Yeul MaPubMed 38132466
- International journal of molecular sciences 24:2023GSK2656157, a PERK Inhibitor, Alleviates Pyroptosis of Macrophages Induced by Mycobacterium Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBoli Ma,Xueyi Nie,Lei Liu,Mengyuan Li,Qi Chen,Yueyang Liu,Yuxin Hou,Yi Yang,Jinrui XuPubMed 38003429
- Molecular medicine (Cambridge, Mass.) 29:1372023Resveratrol reduces ROS-induced ferroptosis by activating SIRT3 and compensating the GSH/GPX4 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingjie Wang,Tianli Shen,Jie Lian,Kai Deng,Chao Qu,Enmeng Li,Gan Li,Yiwei Ren,Zijun Wang,Zhengdong Jiang,Xuejun Sun,Xuqi LiPubMed 37858064
- Bioengineering (Basel, Switzerland) 10:2023Reproducibility and Robustness of a Liver Microphysiological System PhysioMimix LC12 under Varying Culture Conditions and Cell Type Combinations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlicia Y Lim,Yuki Kato,Courtney Sakolish,Alan Valdiviezo,Gang Han,Piyush Bajaj,Jason Stanko,Stephen S Ferguson,Remi Villenave,Philip Hewitt,Rhiannon N Hardwick,Ivan RusynPubMed 37892925
- PloS one 18:e02921242023Cinnamaldehyde alleviates doxorubicin-induced cardiotoxicity by decreasing oxidative stress and ferroptosis in cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeijiao Mao,Wang Zheng,Bin Deng,Youhua Wang,Duan Zhou,Lin Shen,Wankang Niku,Na ZhangPubMed 37824478
- Frontiers in molecular biosciences 10:12742982023Hypoxia and lactate influence VOC production in A549 lung cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakeshi Furuhashi,Yuki Matsumoto,Ryuga Ishii,Takehito Sugasawa,Shigenori OtaPubMed 37808517
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Urine (UTI), Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
- Enzyme activity (quantitative)
- Range
- 1 - 100 mU/mL
- Assay time
- 30m
- Sensitivity
- > 1 mU/mL
Associated Products
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Target data
Function
Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Alternative names
PIG19, LDHA, L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, LDH-M
What's included?
Recommended products
Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 is a quantitative, addition-only assay with a single 30-min incubation step. In the assay, LDH reduces NAD, followed by interaction with a probe. Readout on any colorimetric (450 nm) plate reader.
- Cited in over 240 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Urine (UTI), Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
- Enzyme activity (quantitative)
- Range
- 1 - 100 mU/mL
- Assay time
- 30m
- Assay Platform
- Microplate reader
- Sensitivity
- > 1 mU/mL
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, tissue, cells, and culture medium.
In the LDH assay protocol, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (OD max = 450 nm). The kit can detect 1 - 100 mU/mL of LDH directly in samples. The assay is quick, convenient, and sensitive.
LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze every 2-3 min for at least 30 min with microplate reader in kinetic mode at 37°C
Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, tissue, cells, and culture medium.
How the LDH assay works
In the LDH assay protocol, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (OD max = 450 nm). The kit can detect 1 - 100 mU/mL of LDH directly in samples. The assay is quick, convenient, and sensitive.
LDH assay protocol summary
- add samples and standards to wells
- add reaction mix
- analyze every 2-3 min for at least 30 min with microplate reader in kinetic mode at 37°C
Getting the best performance from ab102526
LDH activity is unlikely to be detectable in healthy urine but may be elevated in the presence of infection or disease of the urinary tract.
Related LDH assay kits
To measure LDH release into cell culture medium from cultured cells in a cytoxicity experiment, we recommend LDH assay kit LDH Assay Kit (Cytotoxicity) ab65393, which is designed specifically as a cytotoxicity assay.
This LDH assay kit ab102526 is designed in a more flexible format for use with a variety of sample types (this includes cell culture medium, and this kit may be used in cytotoxicity assays). This flexible format LDH assay kit is also available as a fluorometric LDH assay kit Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) ab197000.
Other notes
This product was previously called K726 Biovision Lactate Dehydrogenase Activity Colorimetric Assay Kit. Abcam acquired Biovision in 2021.
How other researchers have used LDH Assay Kit ab102526
This LDH assay kit has been used in publications in a variety of sample types, including:
- Human: serum1
- Mouse: kidney tissue2, kidney epithelial cell line lysates3, muscle tissue4, serum2,5, vaginal lavage fluid6, liver tissue7
- Rat: H8C2 cell lysates8
It has also been used in LDH release cytotoxicity cell culture assays including in human hippocampal neuronal cultures9, THP-1 cells10, primary chondrocyte cells on a membrane scaffold11, primary lymphocytes12, ovarian cancer cells co-cultured with mouse splenocytes in a cytotoxic T lymphocyte assay13
References: 1-Zhang Z et al 2018, Solivio MJ et al 2016; 2-Niles DJ et al 2018; 3-Tanner LB et al 2018; 4-Tam BT et al 2018; 5-Kim HY et al 2016; 6-Nash EE et al 2016; 7-Lai C et al 2018; 8-Zhou Z et al 2018; 9-Wang Q et al 2019; 10-Alimov I et al 2019; 11-Stocco TD et al 2019; 12-Dreisig K et al 2018; 13-Sun Y et al 2018
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lactate dehydrogenase (LDH) is an enzyme that catalyzes the interconversion of pyruvate and lactate along with the conversion of NADH to NAD+. LDH is known by other names such as lactic acid dehydrogenase and LDH-5. The enzyme has a molecular weight of approximately 36 kDa. LDH exists in almost all tissues having multiple isoforms that are expressed differently depending on the tissue type. It shows high expression in muscle tissue liver and heart indicating its extensive role in energy metabolism.
- Biological function summary
Lactate dehydrogenase plays a critical role in anaerobic glycolysis. The enzyme helps in regenerating NAD+ from NADH allowing glycolysis to continue in the absence of oxygen. LDH is not a part of any larger protein complex working independently to fulfill its function in the glycolytic pathway. It serves in rapid energy production especially under hypoxic or exertional conditions where oxygen supply is limited.
- Pathways
LDH is significantly involved in the glycolysis and gluconeogenesis pathways. Within glycolysis LDH helps facilitate the conversion of pyruvate to lactate during anaerobic conditions a step important for ATP production when oxygen is scarce. The enzyme is tied closely to phosphofructokinase-1 (PFK-1) in glycolysis given that both enzymes are central to maintaining the glycolytic flow. In gluconeogenesis though functionally reversed from its role in glycolysis LDH helps to manage lactate removal an important step for glucose synthesis from non-carbohydrate sources.
- Associated diseases and disorders
Lactate dehydrogenase levels often act as a biomarker for tissue damage or certain cancers as its release into the bloodstream signals cellular injury or death. Elevated LDH levels are associated with conditions like myocardial infarction and certain forms of anemia. In cancer such as lymphoma or leukemia LDH correlates with the progression of the disease and acts as a prognostic marker. LDH's connection to these conditions often leads to insights into disease severity and progression due to its association with proteins like p53 and HIF-1 which play roles in cellular metabolism and hypoxia response.
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6 product images
Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)
Kinetic profiles of approx 0.5 mU of a sample of pure LDH (Positive Control) and 2 μL frozen human serum using buffer as a background control.
Tam BT et al. 2018 Sci Rep 8:3689 Use licensed under: http://creativecommons.org/licenses/by/4.0/Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)
LDH assay on mouse skeletal muscle.
Tam BT et al. used the LDH assay kit on mouse skeletal muscle tissue lysates comparing pressure-induced compressed and control samples across a range of knockout mice lines.
Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)
NADH Standard Curve.
Image from Xu Y et al., PLoS One. 2014;9(6):e100127. Fig 2(B).; doi: 10.1371/journal.pone.0100127. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)
Cellular necrosis was measured as LDH release after cisplatin treatment for 24 h and 48 h using ab102526. The release of LDH was apparently increased at 24 and 48 h after cisplatin treatment.
Enzyme activity assay - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)
Alt-Aissa et al. used Pyruvate Kinase Assay Kit Pyruvate Kinase Assay Kit ab83432 and LDH Assay Kit ab102526 to investigate changes in glycolytic flux in human hearts in coronary heart disease.
Glycolytic pathway in human hearts. (A) Pyruvate Kinase Activity and (B) Lactate Dehydrogenase Activity were measured in lysates of left ventricles of human hearts; Values are expressed as mean +/- SEM expressed as fold change relative to Non-CAD heart lysates; n = 8 in each group; *p < 0.05 vs Non-CAD hearts.
Pyruvate kinase activity was measured in heart lysates from CAD and non-CAD patients. The experiments were performed using the Pyruvate Kinase Assay Kit (Pyruvate Kinase Assay Kit ab83432, Abcam, Cambridge, MA, USA) according to the manufacturer instructions.
Lactate Dehydrogenase activity (LDH) was measured in heart lysates from CAD and non-CAD patients. The experiments were performed using the LDH Kit (ab102526, Abcam, Cambridge, MA, USA) according to the manufacturer instructions.
Biochemical assay - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)
Diagram showing the principles of the Lactate Dehydrogenase assay method.
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