LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric)

Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, tissue, cells, and culture medium.

In the LDH assay protocol, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (OD max = 450 nm). The kit can detect 1 - 100 mU/mL of LDH directly in samples. The assay is quick, convenient, and sensitive.

LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze every 2-3 min for at least 30 min with microplate reader in kinetic mode at 37°C

Technical Note: LDH activity is unlikely to be detectable in healthy urine but may be elevated in the presence of infection or disease of the urinary tract.

This product is manufactured by BioVision, an Abcam company and was previously called K726 Lactate Dehydrogenase Activity Colorimetric Assay Kit. K726-500 is the same size as the 500 test size of ab102526.

To measure LDH release into cell culture medium from cultured cells in a cytoxicity experiment, we recommend LDH assay kit LDH Assay Kit (Cytotoxicity) ab65393, which is designed specifically as a cytotoxicity assay.

This LDH assay kit ab102526 is designed in a more flexible format for use with a variety of sample types (this includes cell culture medium, and this kit may be used in cytotoxicity assays). This flexible format LDH assay kit is also available as a fluorometric LDH assay kit ab197000.

How other researchers have used LDH Assay Kit ab102526

This LDH assay kit has been used in publications in a variety of sample types, including:
- Human: serum¹
- Mouse: kidney tissue², kidney epithelial cell line lysates³, muscle tissue⁴, serum^2,5, vaginal lavage fluid⁶, liver tissue⁷
- Rat: H8C2 cell lysates⁸

It has also been used in LDH release cytotoxicity cell culture assays including in human hippocampal neuronal cultures⁹, THP-1 cells¹⁰, primary chondrocyte cells on a membrane scaffold¹¹, primary lymphocytes¹², ovarian cancer cells co-cultured with mouse splenocytes in a cytotoxic T lymphocyte assay¹³

^{References: 1-Zhang Z et al 2018, Solivio MJ et al 2016; 2-Niles DJ et al 2018; 3-Tanner LB et al 2018; 4-Tam BT et al 2018; 5-Kim HY et al 2016; 6-Nash EE et al 2016; 7-Lai C et al 2018; 8-Zhou Z et al 2018; 9-Wang Q et al 2019; 10-Alimov I et al 2019; 11-Stocco TD et al 2019; 12-Dreisig K et al 2018; 13-Sun Y et al 2018}