Skip to main content

Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, tissue, cells, and culture medium.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.


Images

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Assay type

Enzyme activity (quantitative)

Range

1 - 100 mU/mL

Assay time

30m

Sensitivity

> 1 mU/mL

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Alternative names

What's included?

500 Test
Components
LDH Assay Buffer
1 x 50 mL
LDH Positive Control
1 x 1 Vial
NADH Standard I
1 x 1 Vial
Substrate Mix
1 x 1 Vial

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, tissue, cells, and culture medium.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Assay type

Enzyme activity (quantitative)

Range

1 - 100 mU/mL

Assay time

30m

Assay Platform

Microplate reader

Sensitivity

> 1 mU/mL

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, tissue, cells, and culture medium.

In the LDH assay protocol, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (OD max = 450 nm). The kit can detect 1 - 100 mU/mL of LDH directly in samples. The assay is quick, convenient, and sensitive.

LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze every 2-3 min for at least 30 min with microplate reader in kinetic mode at 37°C

This product is manufactured by BioVision, an Abcam company and was previously called K726 Lactate Dehydrogenase Activity Colorimetric Assay Kit. K726-500 is the same size as the 500 test size of ab102526.

To measure LDH release into cell culture medium from cultured cells in a cytoxicity experiment, we recommend LDH assay kit ab65393, which is designed specifically as a cytotoxicity assay.

This LDH assay kit ab102526 is designed in a more flexible format for use with a variety of sample types (this includes cell culture medium, and this kit may be used in cytotoxicity assays). This flexible format LDH assay kit is also available as a fluorometric LDH assay kit ab197000.

How other researchers have used LDH Assay Kit ab102526

This LDH assay kit has been used in publications in a variety of sample types, including:
- Human: serum1
- Mouse: kidney tissue2, kidney epithelial cell line lysates3, muscle tissue4, serum2,5, vaginal lavage fluid6, liver tissue7
- Rat: H8C2 cell lysates8

It has also been used in LDH release cytotoxicity cell culture assays including in human hippocampal neuronal cultures9, THP-1 cells10, primary chondrocyte cells on a membrane scaffold11, primary lymphocytes12, ovarian cancer cells co-cultured with mouse splenocytes in a cytotoxic T lymphocyte assay13

References: 1-Zhang Z et al 2018, Solivio MJ et al 2016; 2-Niles DJ et al 2018; 3-Tanner LB et al 2018; 4-Tam BT et al 2018; 5-Kim HY et al 2016; 6-Nash EE et al 2016; 7-Lai C et al 2018; 8-Zhou Z et al 2018; 9-Wang Q et al 2019; 10-Alimov I et al 2019; 11-Stocco TD et al 2019; 12-Dreisig K et al 2018; 13-Sun Y et al 2018

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526), expandable thumbnail
    Tam BT et al. 2018 Sci Rep 8:3689 Use licensed under: http://creativecommons.org/licenses/by/4.0/

    Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)

    LDH assay on mouse skeletal muscle.

    Tam BT et al. used the LDH assay kit on mouse skeletal muscle tissue lysates comparing pressure-induced compressed and control samples across a range of knockout mice lines.

  • Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526), expandable thumbnail

    Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)

    Kinetic profiles of approx 0.5 mU of a sample of pure LDH (Positive Control) and 2 μL frozen human serum using buffer as a background control.

  • Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526), expandable thumbnail

    Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)

    NADH Standard Curve.

  • Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526), expandable thumbnail
    Image from Xu Y et al., PLoS One. 2014;9(6):e100127. Fig 2(B).; doi: 10.1371/journal.pone.0100127. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)

    Cellular necrosis was measured as LDH release after cisplatin treatment for 24 h and 48 h using ab102526. The release of LDH was apparently increased at 24 and 48 h after cisplatin treatment.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com