LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric)

• Biochemical assay

Lab

Biochemical assay - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (AB102526)

Diagram showing the principles of the Lactate Dehydrogenase assay method.

• FuncS

Supplier Data

Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (AB102526)

NADH Standard Curve.

• FuncS

PubMed

Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (AB102526)

LDH assay on mouse skeletal muscle.

Tam BT et al. used the LDH assay kit on mouse skeletal muscle tissue lysates comparing pressure-induced compressed and control samples across a range of knockout mice lines.

Tam BT et al. 2018 Sci Rep 8:3689 Use licensed under: http://creativecommons.org/licenses/by/4.0/

• FuncS

Supplier Data

Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (AB102526)

Kinetic profiles of approx 0.5 mU of a sample of pure LDH (Positive Control) and 2 μL frozen human serum using buffer as a background control.

• FuncS

PubMed

Functional Studies - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (AB102526)

Cellular necrosis was measured as LDH release after cisplatin treatment for 24 h and 48 h using ab102526. The release of LDH was apparently increased at 24 and 48 h after cisplatin treatment.

Image from Xu Y et al., PLoS One. 2014;9(6):e100127. Fig 2(B).; doi: 10.1371/journal.pone.0100127. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• Enzyme activity assay

PubMed

Enzyme activity assay - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (AB102526)

Alt-Aissa et al. used Pyruvate Kinase Assay Kit ab83432 and LDH Assay Kit ab102526 to investigate changes in glycolytic flux in human hearts in coronary heart disease.

Glycolytic pathway in human hearts. (A) Pyruvate Kinase Activity and (B) Lactate Dehydrogenase Activity were measured in lysates of left ventricles of human hearts; Values are expressed as mean +/- SEM expressed as fold change relative to Non-CAD heart lysates; n = 8 in each group; *p < 0.05 vs Non-CAD hearts.

Pyruvate kinase activity was measured in heart lysates from CAD and non-CAD patients. The experiments were performed using the Pyruvate Kinase Assay Kit (ab83432, Abcam, Cambridge, MA, USA) according to the manufacturer instructions.

Lactate Dehydrogenase activity (LDH) was measured in heart lysates from CAD and non-CAD patients. The experiments were performed using the LDH Kit (ab102526, Abcam, Cambridge, MA, USA) according to the manufacturer instructions.

• Other

Supplier Data

Other - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (AB102526)

Representative image of LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) ab102526

Components shown from left to right :

- LDH Positive Control

- NADH Standard I

- Substrate Mix

- LDH Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.