LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric)

Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ab102526 quantifies LDH activity in a variety of samples such as serum or plasma, tissue, cells, and culture medium.

In the LDH assay protocol, LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (OD max = 450 nm). The kit can detect 1 - 100 mU/mL of LDH directly in samples. The assay is quick, convenient, and sensitive.

LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze every 2-3 min for at least 30 min with microplate reader in kinetic mode at 37°C

This product is manufactured by BioVision, an Abcam company and was previously called K726 Lactate Dehydrogenase Activity Colorimetric Assay Kit. K726-500 is the same size as the 500 test size of ab102526.

To measure LDH release into cell culture medium from cultured cells in a cytoxicity experiment, we recommend LDH assay kit ab65393, which is designed specifically as a cytotoxicity assay.

This LDH assay kit ab102526 is designed in a more flexible format for use with a variety of sample types (this includes cell culture medium, and this kit may be used in cytotoxicity assays). This flexible format LDH assay kit is also available as a fluorometric LDH assay kit ab197000.

How other researchers have used LDH Assay Kit ab102526

This LDH assay kit has been used in publications in a variety of sample types, including:
- Human: serum¹
- Mouse: kidney tissue², kidney epithelial cell line lysates³, muscle tissue⁴, serum^2,5, vaginal lavage fluid⁶, liver tissue⁷
- Rat: H8C2 cell lysates⁸

It has also been used in LDH release cytotoxicity cell culture assays including in human hippocampal neuronal cultures⁹, THP-1 cells¹⁰, primary chondrocyte cells on a membrane scaffold¹¹, primary lymphocytes¹², ovarian cancer cells co-cultured with mouse splenocytes in a cytotoxic T lymphocyte assay¹³

^{References: 1-Zhang Z et al 2018, Solivio MJ et al 2016; 2-Niles DJ et al 2018; 3-Tanner LB et al 2018; 4-Tam BT et al 2018; 5-Kim HY et al 2016; 6-Nash EE et al 2016; 7-Lai C et al 2018; 8-Zhou Z et al 2018; 9-Wang Q et al 2019; 10-Alimov I et al 2019; 11-Stocco TD et al 2019; 12-Dreisig K et al 2018; 13-Sun Y et al 2018}