AB197004

LDH-Cytotoxicity Assay Kit (Fluorometric)

Name: LDH-Cytotoxicity Assay Kit (Fluorometric) (ab197004) | Abcam
Brand: Abcam Limited
SKU: AB197004
Price: 520 USD
Availability: InStock

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(19 Publications)

LDH Assay Kit (Cytotoxicity, Fluorometric) (ab197004) provides a sensitive, quick, and easy way to detect LDH (lactate dehydrogenase) released from damaged cells.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

PIG19, LDHA, L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, LDH-M

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Functional Studies - LDH-Cytotoxicity Assay Kit (Fluorometric) (AB197004)

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Supplier Data

Functional Studies - LDH-Cytotoxicity Assay Kit (Fluorometric) (AB197004)

Relative Fluorescence Units of untreated Jurkat cells or treated with Cell Lysis Solution.

Relative fluorescence units of untreated Jurkat cells (low control) or treated with Cell Lysis Solution for 30 min (high control).

FuncS

Supplier Data

Functional Studies - LDH-Cytotoxicity Assay Kit (Fluorometric) (AB197004)

Overnight treatment of HeLa cells with staurosporine or cycloheximide.

Overnight treatment of HeLa cells with 100μM of staurosporine or 3μM of cycloheximide. LDH released into the medium was measured along with blank, untreated cells (low control), LDH Positive Control, and lysed cells (high control)

FuncS

Supplier Data

Functional Studies - LDH-Cytotoxicity Assay Kit (Fluorometric) (AB197004)

Untreated Jurkat cells or treated with Cell Lysis Solution.

Untreated Jurkat cells (low control) or treated with Cell Lysis Solution for 30 min (high control).

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Enzyme activity

Results type

Quantitative

Sensitivity

= 100 Cells/well

Assay time

20m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

LDH Assay Kit (Cytotoxicity, Fluorometric) (ab197004) provides a sensitive, quick, and easy way to detect LDH (lactate dehydrogenase) released from damaged cells.

In the LDH assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm).

The amount of fluorescence is directly proportional to the number of damaged cells.

The assay is adaptable to high-throughput format and can be completed in less than 20 min. Sensitivity: ~ 100 cells. 5ul of cell culture media required per assay.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lactate dehydrogenase (LDH) is an enzyme that catalyzes the interconversion of pyruvate and lactate along with the conversion of NADH to NAD+. LDH is known by other names such as lactic acid dehydrogenase and LDH-5. The enzyme has a molecular weight of approximately 36 kDa. LDH exists in almost all tissues having multiple isoforms that are expressed differently depending on the tissue type. It shows high expression in muscle tissue liver and heart indicating its extensive role in energy metabolism.

Biological function summary

Lactate dehydrogenase plays a critical role in anaerobic glycolysis. The enzyme helps in regenerating NAD+ from NADH allowing glycolysis to continue in the absence of oxygen. LDH is not a part of any larger protein complex working independently to fulfill its function in the glycolytic pathway. It serves in rapid energy production especially under hypoxic or exertional conditions where oxygen supply is limited.

Pathways

LDH is significantly involved in the glycolysis and gluconeogenesis pathways. Within glycolysis LDH helps facilitate the conversion of pyruvate to lactate during anaerobic conditions a step important for ATP production when oxygen is scarce. The enzyme is tied closely to phosphofructokinase-1 (PFK-1) in glycolysis given that both enzymes are central to maintaining the glycolytic flow. In gluconeogenesis though functionally reversed from its role in glycolysis LDH helps to manage lactate removal an important step for glucose synthesis from non-carbohydrate sources.

Lactate dehydrogenase levels often act as a biomarker for tissue damage or certain cancers as its release into the bloodstream signals cellular injury or death. Elevated LDH levels are associated with conditions like myocardial infarction and certain forms of anemia. In cancer such as lymphoma or leukemia LDH correlates with the progression of the disease and acts as a prognostic marker. LDH's connection to these conditions often leads to insights into disease severity and progression due to its association with proteins like p53 and HIF-1 which play roles in cellular metabolism and hypoxia response.

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Product protocols

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Target data

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).

See full target information LDHA

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Biomedical microdevices 26:32 PubMed38963644

2024

A dynamic flow fetal membrane organ-on-a-chip system for modeling the effects of amniotic fluid motion.

Applications

Unspecified application

Species

Unspecified reactive species

Sungjin Kim,Po Yi Lam,Lauren S Richardson,Ramkumar Menon,Arum Han

Nature communications 14:5576 PubMed37696888

2023

A magneto-activated nanoscale cytometry platform for molecular profiling of small extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Kangfu Chen,Bill T V Duong,Sharif U Ahmed,Piriththiv Dhavarasa,Zongjie Wang,Mahmoud Labib,Connor Flynn,Jingya Xu,Yi Y Zhang,Hansen Wang,Xiaolong Yang,Jagotamoy Das,Hossein Zargartalebi,Yuan Ma,Shana O Kelley

Journal of diabetes research 2023:7423661 PubMed37261217

2023

Astragaloside IV Attenuates High-Glucose-Induced Impairment in Diabetic Nephropathy by Increasing Klotho Expression via the NF-B/NLRP3 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin He,Jialin Cui,Yimin Shi,Tao Wang,Junyan Xin,Yimeng Li,Xiaomeng Shan,Zhiyao Zhu,Yanbin Gao

The Tohoku journal of experimental medicine 260:205-214 PubMed37019647

2023

MiR-10b-5p Impairs TET2-Mediated Inhibition of PD-L1 Transcription Thus Promoting Immune Evasion and Tumor Progression in Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Du,Dayu Chen,Ke Wei,Duo Yu,Zhiqiang Gan,Guozheng Xu,Guojie Yao

BMC anesthesiology 22:304 PubMed36163004

2022

Dexmedetomidine pretreatment alleviates ropivacaine-induced neurotoxicity via the miR-10b-5p/BDNF axis.

Applications

Unspecified application

Species

Unspecified reactive species

Weicai Xu,Xiaojun Li,Long Chen,Xiaopan Luo,Sheliang Shen,Jing Wang

Advanced healthcare materials 11:e2201203 PubMed35856921

2022

Small Extracellular Vesicles Secreted by Nigrostriatal Astrocytes Rescue Cell Death and Preserve Mitochondrial Function in Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Loredana Leggio,Francesca L'Episcopo,Andrea Magrì,María José Ulloa-Navas,Greta Paternò,Silvia Vivarelli,Carlos A P Bastos,Cataldo Tirolo,Nunzio Testa,Salvatore Caniglia,Pierpaolo Risiglione,Fabrizio Pappalardo,Alessandro Serra,Patricia García-Tárraga,Nuno Faria,Jonathan J Powell,Luca Peruzzotti-Jametti,Stefano Pluchino,José Manuel García-Verdugo,Angela Messina,Bianca Marchetti,Nunzio Iraci

Immunity, inflammation and disease 10:e641 PubMed35759233

2022

TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Sun,Xiaobing Chen,Yongpeng Xie,Yanli Wang,Qian Zhang,Yu Lu,Xiaomin Li

Molecules (Basel, Switzerland) 27: PubMed35566175

2022

nurP28, a New-to-Nature Zein-Derived Peptide, Enhances the Therapeutic Effect of Docetaxel in Breast Cancer Monolayers and Spheroids.

Applications

Unspecified application

Species

Unspecified reactive species

Plinio Alejandro Trinidad-Calderón,Laura Margarita López-Castillo,Salvador Gallegos-Martínez,Grissel Trujillo-de Santiago,Silverio García-Lara,Mario Moisés Álvarez

Pharmaceutical biology 60:56-64 PubMed34905418

2021

Myristicin regulates proliferation and apoptosis in oxidized low-density lipoprotein-stimulated human vascular smooth muscle cells and human umbilical vein endothelial cells by regulating the PI3K/Akt/NF-κB signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Luo,Huiying Liang,Luoying Liu

Experimental and therapeutic medicine 22:1398 PubMed34650646

2021

Roflumilast, a type of phosphodiesterase 4 inhibitor, can reduce intestinal injury caused by sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyuan Zhang,Meifeng Liang,Xiongfei Wan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

LDH-Cytotoxicity Assay Kit (Fluorometric)

Functional Studies - LDH-Cytotoxicity Assay Kit (Fluorometric) (AB197004)

Functional Studies - LDH-Cytotoxicity Assay Kit (Fluorometric) (AB197004)

Functional Studies - LDH-Cytotoxicity Assay Kit (Fluorometric) (AB197004)

Key facts

Detection method

Sample types

Assay type

Results type

Sensitivity

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise