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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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LDL Uptake Assay Kit ab287862 contains fluorophore-labeled human LDL that can be detected when taken up by cells. Readout is by fluorotric plate reader (Ex/Em = 488/523 nm).
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.
Fluorescent
Adherent cells
Cell-based (quantitative)
LDL Uptake Assay Kit ab287862 contains fluorophore-labeled human LDL that can be detected when taken up by cells. Readout is by fluorotric plate reader (Ex/Em = 488/523 nm).
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.
Fluorescent
Adherent cells
Cell-based (quantitative)
Microplate (12 x 8 well strips)
Blue Ice
Multi
Multi
Please refer to protocols
Low-density lipoprotein (LDL) is a large spherical macromolecule that serves as a 'shuttle' to transport hydrophobic molecules (lipids and cholesterol) throughout the body via the aqueous environment of the bloodstream. The LDL Uptake Assay Kit (Cell-Based) ab287862 (Preivously known as LDL Uptake Assay Kit (Cell-Based) K436) enables measurement of receptor-mediated LDL uptake by various cell types. The kit contains fluorescent human LDL, which is covalently-labeled with a bright, highly photostable fluorophore that can be detected when taken up by cells (Ex/Em = 488/523 nm). Unlabeled human LDL is included in the kit for validation of specific labeled LDL uptake.
This product is manufactured by BioVision, an Abcam company and was previously called K436 LDL Uptake Assay Kit (Cell-Based). K436-100 is the same size as the 96 test size of ab287862.
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Full details and terms and conditions can be found here:
Terms & Conditions.
LDL uptake cell imaging.
Fluorescence imaging showing the extent of fluorescent LDL uptake in HepG2 cells after 4 hours. (i-ii) phase contrast and fluorescence images of cells treated with labeled LDL (10 μg), (iii-iv) phase contrast and fluorescence images of cells treated with Fluorophore-Labeled LDL (10 μg) and an excess of unlabeled LDL to outcompete labeled uptake by saturating LDL receptors. Cells were grown on the included polylysine-coated 96-well plate; fluorescence images were obtained with a Nikon TE2000 inverted microscope using a 20X Plan Fluor objective and a FITC filter cube.
Labeled LDL uptake in HepG2 cells.
Cells were cholesterol-starved overnight in culture medium containing 1% lipoprotein-deficient serum, then incubated with Fluorophore-Labeled LDL (10 μg/well) for 4 hours. Specificity of labeled (Fl-LDL) uptake was determined by competition with excess unlabeled LDL (Fl-LDL + Unlabeled). To account for any potential non-specific binding of labeled LDL (in the absence of functional uptake), cells were incubated with Fluorophore-Labeled LDL (10μg/well) for 2 min, followed by aspiration and rinsing with Assay Buffer (Wash Off). Uptake data are presented as mean LDL mass/hour per 104 cells ± SD, with each data point representing the mean of ≥5 wells.
Fluorophore-Labeled LDL Standard curve.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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