LDL Uptake Assay Kit Cell-Based

3 product images

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  Fluorescent Cell Imaging - LDL Uptake Assay Kit Cell-Based (ab287862)

  LDL uptake cell imaging.

  Fluorescence imaging showing the extent of fluorescent LDL uptake in HepG2 cells after 4 hours. (i-ii) phase contrast and fluorescence images of cells treated with labeled LDL (10 μg), (iii-iv) phase contrast and fluorescence images of cells treated with Fluorophore-Labeled LDL (10 μg) and an excess of unlabeled LDL to outcompete labeled uptake by saturating LDL receptors. Cells were grown on the included polylysine-coated 96-well plate; fluorescence images were obtained with a Nikon TE2000 inverted microscope using a 20X Plan Fluor objective and a FITC filter cube.

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  Cellular Activity - LDL Uptake Assay Kit Cell-Based (ab287862)

  Labeled LDL uptake in HepG2 cells.

  Cells were cholesterol-starved overnight in culture medium containing 1% lipoprotein-deficient serum, then incubated with Fluorophore-Labeled LDL (10 μg/well) for 4 hours. Specificity of labeled (Fl-LDL) uptake was determined by competition with excess unlabeled LDL (Fl-LDL + Unlabeled). To account for any potential non-specific binding of labeled LDL (in the absence of functional uptake), cells were incubated with Fluorophore-Labeled LDL (10μg/well) for 2 min, followed by aspiration and rinsing with Assay Buffer (Wash Off). Uptake data are presented as mean LDL mass/hour per 10⁴ cells ± SD, with each data point representing the mean of ≥5 wells.

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  Cellular Activity - LDL Uptake Assay Kit Cell-Based (ab287862)

  Fluorophore-Labeled LDL Standard curve.