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LDL Uptake Assay Kit ab287862 contains fluorophore-labeled human LDL that can be detected when taken up by cells. Readout is by fluorotric plate reader (Ex/Em = 488/523 nm).

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

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Images

Key facts

Detection method

Fluorescent

Sample types

Adherent cells

Assay type

Cell-based (quantitative)

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What's included?

96 Test
Components
Fluorophore-Labeled LDL
1 x 250 µL
Polylysine Coated Cell Culture Plate
1 x 1 Unit
Unlabeled LDL
1 x 100 µL
Uptake Assay Buffer
1 x 50 mL

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LDL Uptake Assay Kit ab287862 contains fluorophore-labeled human LDL that can be detected when taken up by cells. Readout is by fluorotric plate reader (Ex/Em = 488/523 nm).

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Fluorescent

Sample types

Adherent cells

Assay type

Cell-based (quantitative)

Assay Platform

Microplate (12 x 8 well strips)

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Low-density lipoprotein (LDL) is a large spherical macromolecule that serves as a 'shuttle' to transport hydrophobic molecules (lipids and cholesterol) throughout the body via the aqueous environment of the bloodstream. The LDL Uptake Assay Kit (Cell-Based) ab287862 (Preivously known as LDL Uptake Assay Kit (Cell-Based) K436) enables measurement of receptor-mediated LDL uptake by various cell types. The kit contains fluorescent human LDL, which is covalently-labeled with a bright, highly photostable fluorophore that can be detected when taken up by cells (Ex/Em = 488/523 nm). Unlabeled human LDL is included in the kit for validation of specific labeled LDL uptake.

This product is manufactured by BioVision, an Abcam company and was previously called K436 LDL Uptake Assay Kit (Cell-Based). K436-100 is the same size as the 96 test size of ab287862.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Fluorescent Cell Imaging - LDL Uptake Assay Kit Cell-Based (ab287862), expandable thumbnail

    Fluorescent Cell Imaging - LDL Uptake Assay Kit Cell-Based (ab287862)

    LDL uptake cell imaging.

    Fluorescence imaging showing the extent of fluorescent LDL uptake in HepG2 cells after 4 hours. (i-ii) phase contrast and fluorescence images of cells treated with labeled LDL (10 μg), (iii-iv) phase contrast and fluorescence images of cells treated with Fluorophore-Labeled LDL (10 μg) and an excess of unlabeled LDL to outcompete labeled uptake by saturating LDL receptors. Cells were grown on the included polylysine-coated 96-well plate; fluorescence images were obtained with a Nikon TE2000 inverted microscope using a 20X Plan Fluor objective and a FITC filter cube.

  • Cellular Activity - LDL Uptake Assay Kit Cell-Based (ab287862), expandable thumbnail

    Cellular Activity - LDL Uptake Assay Kit Cell-Based (ab287862)

    Labeled LDL uptake in HepG2 cells.

    Cells were cholesterol-starved overnight in culture medium containing 1% lipoprotein-deficient serum, then incubated with Fluorophore-Labeled LDL (10 μg/well) for 4 hours. Specificity of labeled (Fl-LDL) uptake was determined by competition with excess unlabeled LDL (Fl-LDL + Unlabeled). To account for any potential non-specific binding of labeled LDL (in the absence of functional uptake), cells were incubated with Fluorophore-Labeled LDL (10μg/well) for 2 min, followed by aspiration and rinsing with Assay Buffer (Wash Off). Uptake data are presented as mean LDL mass/hour per 104 cells ± SD, with each data point representing the mean of ≥5 wells.

  • Cellular Activity - LDL Uptake Assay Kit Cell-Based (ab287862), expandable thumbnail

    Cellular Activity - LDL Uptake Assay Kit Cell-Based (ab287862)

    Fluorophore-Labeled LDL Standard curve.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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