LDL Uptake Assay Kit (Flow cytometry)
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(3 Publications)
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Functional Studies - LDL Uptake Assay Kit (Flow cytometry) (AB236208)
Example flow cytometric analysis.
HepG2 cells were plated at 2 x 105 cells/well in a 24 well plate approximately 48 hours before addition of LDL-DyLightTM 488. After a four hour incubation with the LDL probe, cells were trypsinized, washed and stained with 7-AAD prior to flow cytometry. In this example analysis, after digital compensation, 7-AAD negative live cells are gated first, followed by scatter. Single cells are gated using an area versus height dot plot, and the geometric mean fluorescence intensity (MFI) of the resulting cells in the LDL-DyLightTM 488 channel is determined.
- FuncS
Supplier Data
Functional Studies - LDL Uptake Assay Kit (Flow cytometry) (AB236208)
Lovastatin increases uptake of LDL.
HepG2 cells were plated at 2 x 105 cells/well in a 24 well plate and allowed to adhere overnight, before being treated with 1 μM Lovastatin or left untreated for 24 hours in MEM + 2% FBS. The final four hours of treatment included the probe LDL-DyLightTM 488. Cells were processed as described in the kit booklet and the flow cytometric data were analyzed as described in Figure 1. Average geometric mean fluorescence intestities (MFI) for each group are plotted.
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Biological function summary
LDL plays an important role by supplying cholesterol to cells which is critical for membrane structure and hormone synthesis. LDL binds to specific receptors on the cell surface prompting its cellular uptake through the LDL uptake assay mechanisms including receptor-mediated endocytosis. This process involves the LDL protein engaging with a receptor complex facilitating the internalization of cholesterol for cell usage or storage. Native LDL can be distinguished from oxidized forms which have different roles in health and disease.
Pathways
LDL is vital in cholesterol homeostasis and lipid metabolism pathways. One notable pathway includes its interaction in the mevalonate pathway essential for cholesterol biosynthesis. The interaction with the insulin signaling pathway also highlights the significance of LDL in energy homeostasis. Related proteins such as high-density lipoprotein (HDL) and very low-density lipoprotein (VLDL) work in tandem with LDL forming a complex system for managing lipid transport and affecting cholesterol levels.
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Publications (3)
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Scientific reports 13:19725 PubMed37957262
2023
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ACS omega 8:30906-30916 PubMed37663489
2023
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iScience 24:103180 PubMed34667945
2021
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