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AB325586

Leukocyte-endothelium Adhesion Assay

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ab325586 Leukocyte-endothelium Adhesion Assay provides a robust system for the quantitative determination of leukocyte-endothelium interactions. The kit contains sufficient reagents for the evaluation of 100 assays in a 96-well plate.
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Functional Studies - Leukocyte-endothelium Adhesion Assay (AB325586)
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Supplier Data

Functional Studies - Leukocyte-endothelium Adhesion Assay (AB325586)

Cytokine Activation of Human Monocytic THP-1 Adhesion to HUVEC. HUVEC monolayer (left) or THP-1 cells (right) were treated with 50 ng/mL TNF-alpha or 1 µM PMA for 12 hrs. Tracker labeled THP-1 cells (50,000 cells/well) were allowed to attach to HUVEC monolayer for 1 hr. Adherent cells were lysed and quantified by as described in the Assay Protocol.

This figure demonstrates typical results with ab325586 Leukocyte-endothelium Adhesion Assay. One should use the data below for reference only. This data should not be used to interpret actual results.

Functional Studies - Leukocyte-endothelium Adhesion Assay (AB325586)
  • FuncS

Supplier Data

Functional Studies - Leukocyte-endothelium Adhesion Assay (AB325586)

Quantitation of Human Monocytic THP-1. Tracker labeled THP-1 cells were titrated in 1X PBS, then subsequently lysed with 2X Lysis Buffer (75 µL of cell suspension was mixed with 75 µL of 2X Lysis Buffer). Fluorescence was quantified as described in Assay Protocol.

This figure demonstrates typical results with ab325586 Leukocyte-endothelium Adhesion Assay. One should use the data below for reference only. This data should not be used to interpret actual results.

Functional Studies - Leukocyte-endothelium Adhesion Assay (AB325586)
  • FuncS

Supplier Data

Functional Studies - Leukocyte-endothelium Adhesion Assay (AB325586)

Human Monocytic THP-1 Adhesion to HUVEC Monolayer. HUVEC monolayer in 48-well plate was treated with 1 µM PMA for 12 hrs. Tracker labeled THP-1 cells were allowed to attach to HUVEC monolayer for 1 hr. Adherent cells were lysed and quantified by as described in the Assay Protocol.

This figure demonstrates typical results with ab325586 Leukocyte-endothelium Adhesion Assay. One should use the data below for reference only. This data should not be used to interpret actual results.

Key facts

Detection method

Fluorometric

Assay type

Cell-based

Results type

Quantitative

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cellular Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Leukocyte extravasation into perivascular tissue plays a key role in inflammatory diseases. This recruitment requires leukocyte interaction with vascular endothelium and consists of multiple,consecutive processes including the capture of circulating leukocytes, subsequent leukocyte rolling, arrest, firm adhesion and transmigration. This multistep paradigm is realized by sequential activation-dependent interactions between endothelial cell adhesion molecules and their specific ligands on leukocytes. The first step of transient adhesion and rolling is known to be mediated by an interaction of leukocyte or endothelial cellselectins and their oligosaccharide-bearing ligands. Arrest and firm adhesion of leukocytes to endothelium is dependent on the activation of β2 integrins like Mac-1 or LFA-1 on the leukocyte cell surface, followed by interaction with endothelial cell proteins belonging to the Ig superfamily such as ICAM-1.
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What's included?

{ "values": { "100Tests": { "sellingSize": "100 Tests", "publicAssetCode":"ab325586-100Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"TNF-alpha", "number":"AB325586-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Gelatin Solution", "number":"AB325586-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer", "number":"AB325586-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"500X Tracker Solution", "number":"AB325586-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"4X Lysis Solution", "number":"AB325586-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
+4°C|-20°C
Appropriate long-term storage conditions
+4°C|-20°C
Storage information
Please refer to protocols
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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