Lipase Assay Kit ab102524 is a rapid, simple, and sensitive colorimetric assay to quantify lipase activity. Readout on any colorimetric (570 nm) plate reader.
- Kit includes Lipase positive control
Images
Publications
Filter by
- Nutrients 15:2023Ketogenic Diet Exacerbates L-Arginine-Induced Acute Pancreatitis and Reveals the Therapeutic Potential of Butyrate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHe Xia,Jing Guo,Jian Shen,Shiman Jiang,Shengyi Han,Lanjuan LiPubMed 37892502
- International journal of molecular sciences 24:2023Stable Enzymatic Nanoparticles from Nucleases, Proteases, Lipase and Antioxidant Proteins with Substrate-Binding and Catalytic Properties.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlga V Morozova,Nikolay A Barinov,Dmitry V KlinovPubMed 36769367
- Microbiology spectrum 11:e02661222023Inhibition of Enveloped Virus Surrogate Phi6 Infection Using Yeast-Derived Vacuoles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWooil Choi,Yang-Hoon Kim,Jiho MinPubMed 36688634
- Nutrients 14:2022and Extracts Improved Lipid Metabolism and Inflammation in High-Energy Diet-Induced Hyperlipidemia Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Tang Tung,Chieh-Hsi Wu,Wen-Chao Chen,Chun-Hsu Pan,Yi-Wen Chen,Shu-Ping Tsao,Chia-Jung Chen,Hui-Yu HuangPubMed 36364926
- Nature communications 13:43272022Targeting de novo lipogenesis and the Lands cycle induces ferroptosis in KRAS-mutant lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaterina Bartolacci,Cristina Andreani,Gonçalo Vale,Stefano Berto,Margherita Melegari,Anna Colleen Crouch,Dodge L Baluya,George Kemble,Kurt Hodges,Jacqueline Starrett,Katerina Politi,Sandra L Starnes,Daniele Lorenzini,Maria Gabriela Raso,Luisa M Solis Soto,Carmen Behrens,Humam Kadara,Boning Gao,Ignacio I Wistuba,John D Minna,Jeffrey G McDonald,Pier Paolo ScaglioniPubMed 35882862
- Frontiers in cell and developmental biology 9:7801422021Treatment of Severe Acute Pancreatitis and Related Lung Injury by Targeting Gasdermin D-Mediated Pyroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinxiang Wu,Jintao Zhang,Jiping Zhao,Shihong Chen,Tao Zhou,Jianwei XuPubMed 34858995
- Nutrients 13:2021Ghee Butter from Bovine Colostrum Reduces Inflammation in the Mouse Model of Acute Pancreatitis with Potential Involvement of Free Fatty Acid Receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAleksandra Tarasiuk,Marcin Talar,Kamila Bulak,Jakub FichnaPubMed 34579147
- Frontiers in nutrition 8:6870562021Exocrine Pancreatic Maturation in Pre-term and Term Piglets Supplemented With Bovine Colostrum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEster Arévalo Sureda,Kateryna Pierzynowska,Björn Weström,Per Torp Sangild,Thomas ThymannPubMed 34249996
- EMBO reports 22:e507672021Endothelial IGF-1 receptor mediates crosstalk with the gut wall to regulate microbiota in obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalie J Haywood,Cheukyau Luk,Katherine I Bridge,Michael Drozd,Natallia Makava,Anna Skromna,Amanda Maccannell,Claire H Ozber,Nele Warmke,Chloe G Wilkinson,Nicole T Watt,Joanna Koch-Paszkowski,Irvin Teh,Jordan H Boyle,Sean Smart,Jurgen E Schneider,Nadira Y Yuldasheva,Lee D Roberts,David J Beech,Piruthivi Sukumar,Stephen B Wheatcroft,Richard M Cubbon,Mark T KearneyPubMed 33934497
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Tissue Extracts, Cell culture media, Cell culture extracts, Milk, Serum
- Assay type
- Enzyme activity
- Assay time
- 1h 30m
- Sensitivity
- = 0.02 mU/well
Associated Products
Select an associated product type
2 products for Alternative Product
Alternative names
lipA, lip, Triacylglycerol lipase, Extracellular lipase, Triacylglycerol ester hydrolase
What's included?
Recommended products
Lipase Assay Kit ab102524 is a rapid, simple, and sensitive colorimetric assay to quantify lipase activity. Readout on any colorimetric (570 nm) plate reader.
- Kit includes Lipase positive control
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Tissue Extracts, Cell culture media, Cell culture extracts, Milk, Serum
- Assay type
- Enzyme activity
- Assay time
- 1h 30m
- Assay Platform
- Microplate reader
- Sensitivity
- = 0.02 mU/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
How the assay works
Lipase assay kit measure the activity of lipase in a sample by using a reaction that produces a color or fluorescence. The amount of the color or fluorescence produced is proportional to the amount of lipase in the sample. In the lipase assay protocol, lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by monitoring a linked change in the absorbance of a probe (OD=570nm). This kit is suitable for high throughput analysis (HTP).
This lipase assay kit detects lipase activity as low as 0.02mU per well.
Lipase Assay Kit (Colorimetric)summary:
- Add samples and standards to wells
- Prepare standard curve
- Add reaction mix
- Measure optical density (OD570 nm) in a kinetic mode for 60-90 min at 37°C on a microplate reader
How other researchers are using
Lipase Assay Kit (Colorimetric) has been used in a variety of sample type including:
Mouse colon and pancreas samples 1
Mouse lung cancer samples 2
Blood samples from mice with pancreatitis 3
References:
1-Xia H et al. 2023
2-Bartolacci C et al. 2022
3-Tarasiuk A et al. 2021
Related and recommended products
See other alternative kits to quantify lipase activity :
- Lipase Assay Kit III (Fluorometric) Lipase Assay Kit III (Fluorometric) ab118969
- Lipase Assay Kit II (Colorimetric) Lipase Assay Kit II (Colorimetric) ab102525
This product is manufactured by BioVision, an Abcam company and was previously called K722 Lipase Activity Colorimetric Assay Kit. K722-100 is the same size as the 100 test size of ab102524.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Functional Studies - Lipase Assay Kit (Colorimetric) (ab102524)
Sample Timeline
Image from Mondragon A et al., PLoS One. 2014;9(8):e104873. Fig 3.; doi: 10.1371/journal.pone.0104873. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - Lipase Assay Kit (Colorimetric) (ab102524)
Plasma lipase levels were measured (using ab102524) after 75 days treatment with saline, liraglutide, exendin-4 or sitagliptin. ND, normal chow diet; HFD, high fat diet. p≤0.05, *; p≤0.01, **, n = 3–7 mice.
There were no detectable differences in plasma lipase activity in mice on a normal chow diet administered any of the three drugs when compared to animals administered saline. Likewise, there was no significant change in plasma lipase activity in mice that were administered saline on a high fat diet vsnormal diet. Furthermore, administration of liraglutide and exendin-4 in combination with a high fat diet also failed to affect plasma lipase activity. We observed no detectable changes in plasma lipase activity in animals maintained on a normal chow diet and administered any of the three drugs when compared to animals administered saline.
Functional Studies - Lipase Assay Kit (Colorimetric) (ab102524)
Glycerol standard Curve
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com