Lipase Assay Kit (Colorimetric)
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(27 Publications)
Lipase Assay Kit ab102524 is a rapid, simple, and sensitive colorimetric assay to quantify lipase activity. Readout on any colorimetric (570 nm) plate reader.
- Kit includes Lipase positive control
View Alternative Names
lipA, lip, Triacylglycerol lipase, Extracellular lipase, Triacylglycerol ester hydrolase
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PubMed
Functional Studies - Lipase Assay Kit (Colorimetric) (AB102524)
Plasma lipase levels were measured (using ab102524) after 75 days treatment with saline, liraglutide, exendin-4 or sitagliptin. ND, normal chow diet; HFD, high fat diet. p≤0.05, *; p≤0.01, **, n = 3–7 mice.
There were no detectable differences in plasma lipase activity in mice on a normal chow diet administered any of the three drugs when compared to animals administered saline. Likewise, there was no significant change in plasma lipase activity in mice that were administered saline on a high fat diet vsnormal diet. Furthermore, administration of liraglutide and exendin-4 in combination with a high fat diet also failed to affect plasma lipase activity. We observed no detectable changes in plasma lipase activity in animals maintained on a normal chow diet and administered any of the three drugs when compared to animals administered saline.
Image from Mondragon A et al., PLoS One. 2014;9(8):e104873. Fig 3.; doi: 10.1371/journal.pone.0104873. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
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Supplier Data
Functional Studies - Lipase Assay Kit (Colorimetric) (AB102524)
Sample Timeline
- FuncS
Supplier Data
Functional Studies - Lipase Assay Kit (Colorimetric) (AB102524)
Glycerol standard Curve
- Schematic Diagram
Supplier Data
Schematic Diagram - Lipase Assay Kit (Colorimetric) (AB102524)
Representative image of Lipase Assay Kit (Colorimetric) ab102524
Components shown from left to right :
- OxiRed Probe
- Lipase Substrate III
- Glycerol Standard
- Enzyme Mix VI
- Lipase Positive Control
- Assay Buffer 5
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
Reactivity data
Product details
How the assay works
Lipase assay kit measure the activity of lipase in a sample by using a reaction that produces a color or fluorescence. The amount of the color or fluorescence produced is proportional to the amount of lipase in the sample. In the lipase assay protocol, lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by monitoring a linked change in the absorbance of a probe (OD=570nm). This kit is suitable for high throughput analysis (HTP).
This lipase assay kit detects lipase activity as low as 0.02mU per well.
Lipase Assay Kit (Colorimetric)summary:
- Add samples and standards to wells
- Prepare standard curve
- Add reaction mix
- Measure optical density (OD570 nm) in a kinetic mode for 60-90 min at 37°C on a microplate reader
How other researchers are using
Lipase Assay Kit (Colorimetric) has been used in a variety of sample type including:
Mouse colon and pancreas samples 1
Mouse lung cancer samples 2
Blood samples from mice with pancreatitis 3
References:
1-Xia H et al. 2023
2-Bartolacci C et al. 2022
3-Tarasiuk A et al. 2021
Related and recommended products
See other alternative kits to quantify lipase activity :
- Lipase Assay Kit III (Fluorometric) ab118969
- Lipase Assay Kit II (Colorimetric) ab102525
This product is manufactured by BioVision, an Abcam company and was previously called K722 Lipase Activity Colorimetric Assay Kit. K722-100 is the same size as the 100 test size of ab102524.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Publications (27)
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Journal of microbiology and biotechnology 35:e2412035 PubMed40081888
2025
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Aquaculture nutrition 2025:7291857 PubMed40066367
2025
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Cell reports. Medicine 5:101525 PubMed38663398
2024
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Nutrients 15: PubMed37892502
2023
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International journal of molecular sciences 24: PubMed36769367
2023
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Microbiology spectrum 11:e0266122 PubMed36688634
2023
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Nutrients 14: PubMed36364926
2022
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Nature communications 13:4327 PubMed35882862
2022
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Frontiers in cell and developmental biology 9:780142 PubMed34858995
2021
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Nutrients 13: PubMed34579147
2021
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com