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AB102524

Lipase Assay Kit (Colorimetric)

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(27 Publications)

Lipase Assay Kit ab102524 is a rapid, simple, and sensitive colorimetric assay to quantify lipase activity. Readout on any colorimetric (570 nm) plate reader.

- Kit includes Lipase positive control

View Alternative Names

lipA, lip, Triacylglycerol lipase, Extracellular lipase, Triacylglycerol ester hydrolase

4 Images
Functional Studies - Lipase Assay Kit (Colorimetric) (AB102524)
  • FuncS

PubMed

Functional Studies - Lipase Assay Kit (Colorimetric) (AB102524)

Plasma lipase levels were measured (using ab102524) after 75 days treatment with saline, liraglutide, exendin-4 or sitagliptin. ND, normal chow diet; HFD, high fat diet. p≤0.05, *; p≤0.01, **, n = 3–7 mice.

There were no detectable differences in plasma lipase activity in mice on a normal chow diet administered any of the three drugs when compared to animals administered saline. Likewise, there was no significant change in plasma lipase activity in mice that were administered saline on a high fat diet vsnormal diet. Furthermore, administration of liraglutide and exendin-4 in combination with a high fat diet also failed to affect plasma lipase activity. We observed no detectable changes in plasma lipase activity in animals maintained on a normal chow diet and administered any of the three drugs when compared to animals administered saline.

Image from Mondragon A et al., PLoS One. 2014;9(8):e104873. Fig 3.; doi: 10.1371/journal.pone.0104873. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Functional Studies - Lipase Assay Kit (Colorimetric) (AB102524)
  • FuncS

Supplier Data

Functional Studies - Lipase Assay Kit (Colorimetric) (AB102524)

Sample Timeline

Functional Studies - Lipase Assay Kit (Colorimetric) (AB102524)
  • FuncS

Supplier Data

Functional Studies - Lipase Assay Kit (Colorimetric) (AB102524)

Glycerol standard Curve

Schematic Diagram - Lipase Assay Kit (Colorimetric) (AB102524)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Lipase Assay Kit (Colorimetric) (AB102524)

Representative image of Lipase Assay Kit (Colorimetric) ab102524

Components shown from left to right :

- OxiRed Probe

- Lipase Substrate III

- Glycerol Standard

- Enzyme Mix VI

- Lipase Positive Control

- Assay Buffer 5

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Extracts, Cell culture media, Cell culture extracts, Milk, Serum

Assay type

Enzyme activity

Sensitivity

= 0.02 mU/well

Assay time

1h 30m

Assay Platform

Microplate reader

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

How the assay works

Lipase assay kit measure the activity of lipase in a sample by using a reaction that produces a color or fluorescence. The amount of the color or fluorescence produced is proportional to the amount of lipase in the sample. In the lipase assay protocol, lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by monitoring a linked change in the absorbance of a probe (OD=570nm). This kit is suitable for high throughput analysis (HTP).

This lipase assay kit detects lipase activity as low as 0.02mU per well.

Lipase Assay Kit (Colorimetric)summary:

- Add samples and standards to wells
- Prepare standard curve
- Add reaction mix
- Measure optical density (OD570 nm) in a kinetic mode for 60-90 min at 37°C on a microplate reader

How other researchers are using

Lipase Assay Kit (Colorimetric) has been used in a variety of sample type including:
Mouse colon and pancreas samples 1
Mouse lung cancer samples 2
Blood samples from mice with pancreatitis 3
References:

1-Xia H et al. 2023
2-Bartolacci C et al. 2022
3-Tarasiuk A et al. 2021

Related and recommended products

See other alternative kits to quantify lipase activity :
- Lipase Assay Kit III (Fluorometric) ab118969
- Lipase Assay Kit II (Colorimetric) ab102525

This product is manufactured by BioVision, an Abcam company and was previously called K722 Lipase Activity Colorimetric Assay Kit. K722-100 is the same size as the 100 test size of ab102524.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab102524-100Test", "assetComponentDetails": [ { "size":"1 x 400 µL", "name":"Lipase Substrate III", "number":"AB102524-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lipase Positive Control", "number":"AB102524-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Enzyme Mix VI", "number":"AB102524-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 5", "number":"AB102524-CMP01", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB102524-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"Glycerol Standard", "number":"AB102524-CMP02", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Catalyzes the hydrolysis of triacylglycerol.
See full target information lip

Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Journal of microbiology and biotechnology 35:e2412035 PubMed40081888

2025

HP7 Improves Gastric Emptying by Modulating Digestive Factors in a Loperamide-Induced Functional Dyspepsia Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Woong Jeong,Daehyeop Lee,Hyeonji Kim,Hyeonjun Gwon,Kippeum Lee,Joo-Yun Kim,Jae-Jung Shim,Jae-Hwan Lee

Aquaculture nutrition 2025:7291857 PubMed40066367

2025

Expanding the Ingredient Basket in Aquaculture: Growth Performance and Feed Utilization of Australian Hybrid Abalone () Fed Methanotrophic Single Cell Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Salini,Weifang Wang,Thomas S Mock,Muhammad A B Siddik,Matthew K Jago,Kelsey L F Bews,David S Francis

Cell reports. Medicine 5:101525 PubMed38663398

2024

α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Debasish Roy,Elliot Dion,Jesse A Sepeda,Juan Peng,Sai Rishik Lingam,Kristy Townsend,Andrew Sas,Wenjing Sun,Andrea Tedeschi

Nutrients 15: PubMed37892502

2023

Ketogenic Diet Exacerbates L-Arginine-Induced Acute Pancreatitis and Reveals the Therapeutic Potential of Butyrate.

Applications

Unspecified application

Species

Unspecified reactive species

He Xia,Jing Guo,Jian Shen,Shiman Jiang,Shengyi Han,Lanjuan Li

International journal of molecular sciences 24: PubMed36769367

2023

Stable Enzymatic Nanoparticles from Nucleases, Proteases, Lipase and Antioxidant Proteins with Substrate-Binding and Catalytic Properties.

Applications

Unspecified application

Species

Unspecified reactive species

Olga V Morozova,Nikolay A Barinov,Dmitry V Klinov

Microbiology spectrum 11:e0266122 PubMed36688634

2023

Inhibition of Enveloped Virus Surrogate Phi6 Infection Using Yeast-Derived Vacuoles.

Applications

Unspecified application

Species

Unspecified reactive species

Wooil Choi,Yang-Hoon Kim,Jiho Min

Nutrients 14: PubMed36364926

2022

and Extracts Improved Lipid Metabolism and Inflammation in High-Energy Diet-Induced Hyperlipidemia Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Tang Tung,Chieh-Hsi Wu,Wen-Chao Chen,Chun-Hsu Pan,Yi-Wen Chen,Shu-Ping Tsao,Chia-Jung Chen,Hui-Yu Huang

Nature communications 13:4327 PubMed35882862

2022

Targeting de novo lipogenesis and the Lands cycle induces ferroptosis in KRAS-mutant lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Caterina Bartolacci,Cristina Andreani,Gonçalo Vale,Stefano Berto,Margherita Melegari,Anna Colleen Crouch,Dodge L Baluya,George Kemble,Kurt Hodges,Jacqueline Starrett,Katerina Politi,Sandra L Starnes,Daniele Lorenzini,Maria Gabriela Raso,Luisa M Solis Soto,Carmen Behrens,Humam Kadara,Boning Gao,Ignacio I Wistuba,John D Minna,Jeffrey G McDonald,Pier Paolo Scaglioni

Frontiers in cell and developmental biology 9:780142 PubMed34858995

2021

Treatment of Severe Acute Pancreatitis and Related Lung Injury by Targeting Gasdermin D-Mediated Pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinxiang Wu,Jintao Zhang,Jiping Zhao,Shihong Chen,Tao Zhou,Jianwei Xu

Nutrients 13: PubMed34579147

2021

Ghee Butter from Bovine Colostrum Reduces Inflammation in the Mouse Model of Acute Pancreatitis with Potential Involvement of Free Fatty Acid Receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandra Tarasiuk,Marcin Talar,Kamila Bulak,Jakub Fichna
View all publications
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