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AB102525

Lipase Assay Kit II (Colorimetric)

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(2 Publications)

Lipase Assay Kit II (Colorimetric) (ab102525) provides a simple, sensitive, and reliable assay for rapid analysis of Lipase activity.
3 Images
Functional Studies - Lipase Assay Kit II (Colorimetric) (AB102525)
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Supplier Data

Functional Studies - Lipase Assay Kit II (Colorimetric) (AB102525)

TNB Standard Curve

Functional Studies - Lipase Assay Kit II (Colorimetric) (AB102525)
  • FuncS

Supplier Data

Functional Studies - Lipase Assay Kit II (Colorimetric) (AB102525)

Lipase Detection in a Sample and Positive Control

Other - Lipase Assay Kit II (Colorimetric) (AB102525)
  • Other

Supplier Data

Other - Lipase Assay Kit II (Colorimetric) (AB102525)

Representative image of Lipase Assay Kit II (Colorimetric) ab102525

Components shown from left to right :

- DTNB

- TNB Standard

- Lipase Substrate II

- Lipase Positive Control

- Assay Buffer 5

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Assay type

Enzyme activity

Assay time

1h 30m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Lipase Assay Kit II (Colorimetric) (ab102525) provides a simple, sensitive, and reliable assay for rapid analysis of Lipase activity.

In the lipase assay, Lipases hydrolyze a specific substrate to generate a product which reacts with the DTNB probe to generate color (412 nm). This kit is suitable for high throughput analysis.

Lipase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader at 3 min and at between 60 and 90 min

Other Notes
This product was previously called K723 Biovision Lipase Activity Colorimetric Assay Kit II. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab102525-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"TNB Standard", "number":"AB102525-CMP05", "productcode":"" }, { "size":"1 x 500 µL", "name":"Lipase Substrate II", "number":"AB102525-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lipase Positive Control", "number":"AB102525-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"DTNB", "number":"AB102525-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 5", "number":"AB102525-CMP01", "productcode":"AB323940" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information Lipase
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aquaculture nutrition 2023:8887768 PubMed37885797

2023

Sustainable Aquafeed Formulations Containing Insect Larval Meal and Grape Marc for the New Zealand Farmed Abalone.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Bullon,Andrea C Alfaro,Moganakumaar Manivannan,Seyedehsara Masoomi Dezfooli,Ali Seyfoddin

Molecular therapy. Nucleic acids 27:870-879 PubMed35141047

2022

Specific adipose tissue gene knockdown prevents diet-induced body weight gain, impacting fat accretion-related gene and protein expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Latorre,Francisco Ortega,Núria Oliveras-Cañellas,Ferran Comas,Aina Lluch,Aleix Gavaldà-Navarro,Samantha Morón-Ros,Wifredo Ricart,Francesc Villarroya,Marta Giralt,José Manuel Fernández-Real,José María Moreno-Navarrete
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com