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Lipase Assay Kit II (Colorimetric) (ab102525) provides a simple, sensitive, and reliable assay for rapid analysis of Lipase activity.

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Images

Functional Studies - Lipase Assay Kit II (Colorimetric) (AB102525), expandable thumbnail
  • Functional Studies - Lipase Assay Kit II (Colorimetric) (AB102525), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
Assay type
Enzyme activity
Assay time
1h 30m

Associated Products

Select an associated product type

1 product for Alternative Product

What's included?

100 Test
Components
Assay Buffer 5
1 x 25 mL
DTNB
1 x 1 Vial
Lipase Positive Control
1 x 1 Vial
Lipase Substrate II
1 x 500 µL
TNB Standard
1 x 1 Vial

Recommended products

Lipase Assay Kit II (Colorimetric) (ab102525) provides a simple, sensitive, and reliable assay for rapid analysis of Lipase activity.

Key facts

Detection method
Colorimetric
Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
Assay type
Enzyme activity
Assay time
1h 30m
Assay Platform
Microplate reader

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Lipase Assay Kit II (Colorimetric) (ab102525) provides a simple, sensitive, and reliable assay for rapid analysis of Lipase activity.

In the lipase assay, Lipases hydrolyze a specific substrate to generate a product which reacts with the DTNB probe to generate color (Absorbance=412 nm). This kit is suitable for high throughput analysis (HTP).

Lipase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader at 3 min and at between 60 and 90 min

This product is manufactured by BioVision, an Abcam company and was previously called K723 Lipase Activity Colorimetric Assay Kit II. K723-100 is the same size as the 100 test size of ab102525.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Lipase Assay Kit II (Colorimetric) (ab102525), expandable thumbnail

    Functional Studies - Lipase Assay Kit II (Colorimetric) (ab102525)

    Lipase Detection in a Sample and Positive Control

  • Functional Studies - Lipase Assay Kit II (Colorimetric) (ab102525), expandable thumbnail

    Functional Studies - Lipase Assay Kit II (Colorimetric) (ab102525)

    TNB Standard Curve

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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