Lipase Activity Assay Kit III (Fluorometric) (ab118969) is a lipase activity assay where lipase hydrolyzes a specific substrate to generate the methylresorufin, which can be detected fluorometrically at Ex/Em=529/600 nm.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Cell stem cell 28:1090-1104.e62021Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Huang,Ridhdhi Desai,Daniel N Conrad,Nayara C Leite,Dipikaa Akshinthala,Christine Maria Lim,Raul Gonzalez,Lakshmi B Muthuswamy,Zev Gartner,Senthil K MuthuswamyPubMed 33915081
- Genes & development 35:117-1322020Differential requirements for MDM2 E3 activity during embryogenesis and in adult mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTimothy J Humpton,Koji Nomura,Julia Weber,Helge M Magnussen,Andreas K Hock,Colin Nixon,Sandeep Dhayade,David Stevenson,Danny T Huang,Douglas Strathdee,Karen Blyth,Karen H VousdenPubMed 33334825
Key facts
- Detection method
Fluorescent
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids
- Assay type
Enzyme activity
- Reactive species
Mammals
- Assay time
1h
- Sensitivity
> 0.0001 mU/well
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Target data
Function
May function as inhibitor of dietary triglyceride digestion. Lacks detectable lipase activity towards triglycerides, diglycerides, phosphatidylcholine, galactolipids or cholesterol esters (in vitro) (By similarity).
Targets
LIPF, PNLIP, LIPC
Alternative names
Inactive pancreatic lipase-related protein 1, PL-RP1, PLRP1, PNLIPRP1
What's included?
Recommended products
Lipase Activity Assay Kit III (Fluorometric) (ab118969) is a lipase activity assay where lipase hydrolyzes a specific substrate to generate the methylresorufin, which can be detected fluorometrically at Ex/Em=529/600 nm.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Inactive pancreatic lipase-related protein 1, PL-RP1, PLRP1, PNLIPRP1
Key facts
- Detection method
Fluorescent
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids
- Assay type
Enzyme activity
- Reactive species
Mammals
- Assay time
1h
- Assay Platform
Microplate reader
- Sensitivity
> 0.0001 mU/well
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Lipase Activity Assay Kit III (Fluorometric) (ab118969) is a lipase activity assay where lipase hydrolyzes a specific substrate to generate the methylresorufin, which can be detected fluorometrically at Ex/Em=529/600 nm. This kit can be used to detect Lipase as low as 0.1 μU/well.
The lipase assay kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput assay of Lipase activity.
Lipase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyse with a microplate reader immediately and at 30-60 min
This product is manufactured by BioVision, an Abcam company and was previously called K724 Lipase Activity Fluorometric Assay Kit III. K724-100 is the same size as the 100 test size of ab118969.
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2 product images
Functional Studies - Lipase Assay Kit III (Fluorometric) (ab118969)
Positive Control and Background assayed using ab118969.
Functional Studies - Lipase Assay Kit III (Fluorometric) (ab118969)
Example of standard curve obtained using ab118969.
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Product protocols
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