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AB118969

Lipase Assay Kit III (Fluorometric)

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(2 Publications)

Lipase Activity Assay Kit III (Fluorometric) (ab118969) is a lipase activity assay where lipase hydrolyzes a specific substrate to generate the methylresorufin, which can be detected fluorometrically at Ex/Em=529/600 nm.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

PLRP1, PNLIPRP1, Inactive pancreatic lipase-related protein 1, PL-RP1, Pancreatic triacylglycerol lipase, PL, PTL, Pancreatic lipase, PNLIP, HTGL, LIPC, Hepatic triacylglycerol lipase, HL, Hepatic lipase, Lipase member C, Lysophospholipase, Phospholipase A1, Gastric triacylglycerol lipase, GL, Gastric lipase, LIPF

3 Images
Functional Studies - Lipase Assay Kit III (Fluorometric) (AB118969)
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Supplier Data

Functional Studies - Lipase Assay Kit III (Fluorometric) (AB118969)

Positive Control and Background assayed using ab118969.

Functional Studies - Lipase Assay Kit III (Fluorometric) (AB118969)
  • FuncS

Supplier Data

Functional Studies - Lipase Assay Kit III (Fluorometric) (AB118969)

Example of standard curve obtained using ab118969.

Schematic Diagram - Lipase Assay Kit III (Fluorometric) (AB118969)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Lipase Assay Kit III (Fluorometric) (AB118969)

Representative image of Lipase Assay Kit III (Fluorometric) ab118969

Components shown from left to right :

- Methylresorufin Standard

- Lipase Substrate I

- Lipase Positive Control

- Assay Buffer 5

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Fluorescent

Sample types

Plasma, Tissue Extracts, Cell culture media, Milk, Serum, Other biological fluids, Cell Lysate

Assay type

Enzyme activity

Sensitivity

> 0.0001 mU/well

Assay time

1h

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Lipase Activity Assay Kit III (Fluorometric) (ab118969) is a lipase activity assay where lipase hydrolyzes a specific substrate to generate the methylresorufin, which can be detected fluorometrically at Ex/Em=529/600 nm. This kit can be used to detect Lipase as low as 0.1 uU/well.

The lipase assay kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput assay of Lipase activity.

Lipase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyse with a microplate reader immediately and at 30-60 min

Other Notes
This product was previously called K724 Biovision Lipase Activity Fluorometric Assay Kit III. Biovision was acquired by Abcam in 2021.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab118969-100Test", "assetComponentDetails": [ { "size":"1 x 40 µL", "name":"Methylresorufin Standard", "number":"AB118969-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"Lipase Substrate I", "number":"AB118969-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lipase Positive Control", "number":"AB118969-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 5", "number":"AB118969-CMP01", "productcode":"AB323940" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

May function as inhibitor of dietary triglyceride digestion. Lacks detectable lipase activity towards triglycerides, diglycerides, phosphatidylcholine, galactolipids or cholesterol esters (in vitro) (By similarity).
See full target information PNLIPRP1

Additional targets

LIPF,PNLIP,LIPC
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell stem cell 28:1090-1104.e6 PubMed33915081

2021

Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Huang,Ridhdhi Desai,Daniel N Conrad,Nayara C Leite,Dipikaa Akshinthala,Christine Maria Lim,Raul Gonzalez,Lakshmi B Muthuswamy,Zev Gartner,Senthil K Muthuswamy

Genes & development 35:117-132 PubMed33334825

2020

Differential requirements for MDM2 E3 activity during embryogenesis and in adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy J Humpton,Koji Nomura,Julia Weber,Helge M Magnussen,Andreas K Hock,Colin Nixon,Sandeep Dhayade,David Stevenson,Danny T Huang,Douglas Strathdee,Karen Blyth,Karen H Vousden
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com