Lipase Activity Assay Kit III (Fluorometric) (ab118969) is a lipase activity assay where lipase hydrolyzes a specific substrate to generate the methylresorufin, which can be detected fluorometrically at Ex/Em=529/600 nm.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Publications
Filter by
- Cell stem cell 28:1090-1104.e62021Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Huang,Ridhdhi Desai,Daniel N Conrad,Nayara C Leite,Dipikaa Akshinthala,Christine Maria Lim,Raul Gonzalez,Lakshmi B Muthuswamy,Zev Gartner,Senthil K MuthuswamyPubMed 33915081
- Genes & development 35:117-1322020Differential requirements for MDM2 E3 activity during embryogenesis and in adult mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTimothy J Humpton,Koji Nomura,Julia Weber,Helge M Magnussen,Andreas K Hock,Colin Nixon,Sandeep Dhayade,David Stevenson,Danny T Huang,Douglas Strathdee,Karen Blyth,Karen H VousdenPubMed 33334825
Key facts
- Detection method
- Fluorescent
- Sample types
- Plasma, Tissue Extracts, Cell culture media, Milk, Serum, Other biological fluids, Cell Lysate
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 1h
- Sensitivity
- > 0.0001 mU/well
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
May function as inhibitor of dietary triglyceride digestion. Lacks detectable lipase activity towards triglycerides, diglycerides, phosphatidylcholine, galactolipids or cholesterol esters (in vitro) (By similarity).
Additional Targets
LIPF, PNLIP, LIPC
Alternative names
PLRP1, PNLIPRP1, Inactive pancreatic lipase-related protein 1, PL-RP1
What's included?
Recommended products
Lipase Activity Assay Kit III (Fluorometric) (ab118969) is a lipase activity assay where lipase hydrolyzes a specific substrate to generate the methylresorufin, which can be detected fluorometrically at Ex/Em=529/600 nm.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Fluorescent
- Sample types
- Plasma, Tissue Extracts, Cell culture media, Milk, Serum, Other biological fluids, Cell Lysate
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 1h
- Assay Platform
- Microplate reader
- Sensitivity
- > 0.0001 mU/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Lipase Activity Assay Kit III (Fluorometric) (ab118969) is a lipase activity assay where lipase hydrolyzes a specific substrate to generate the methylresorufin, which can be detected fluorometrically at Ex/Em=529/600 nm. This kit can be used to detect Lipase as low as 0.1 uU/well.
The lipase assay kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput assay of Lipase activity.
Lipase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyse with a microplate reader immediately and at 30-60 min
Other Notes
This product was previously called K724 Biovision Lipase Activity Fluorometric Assay Kit III. Biovision was acquired by Abcam in 2021.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Lipase Assay Kit III (Fluorometric) (ab118969)
Positive Control and Background assayed using ab118969.
Functional Studies - Lipase Assay Kit III (Fluorometric) (ab118969)
Example of standard curve obtained using ab118969.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com