AB242305

Lipid Assay Kit (unsaturated fatty acids)

Name: Lipid Assay Kit (unsaturated fatty acids) (ab242305) | Abcam
Brand: Abcam Limited
SKU: AB242305
Price: 665 USD
Availability: InStock

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(14 Publications)

Lipid Assay Kit ab242305 quantifies the lipid content (unsaturated fatty acids only) of samples using the sulfo-phospho-vanillin method. Readout on a colorimetric (540 nm) plate reader.

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Functional Studies - Lipid Assay Kit (unsaturated fatty acids) (AB242305)

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Supplier Data

Functional Studies - Lipid Assay Kit (unsaturated fatty acids) (AB242305)

Lipid Quantification Kit Standard Curve

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Supplier Data

Functional Studies - Lipid Assay Kit (unsaturated fatty acids) (AB242305)

Detection of lipids from Human Serum or Human Plasma.

Fifteen microliters of undiluted Human Serum, Human Plasma, or Negative Control buffer were analyzed using ab242305.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue, Cell culture media, Serum

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Product details

Lipid Assay Kit (unsaturated fatty acids) (ab242305) measures the lipid content (unsaturated fatty acids only) of samples using the sulfo-phospho-vanillin method resulting in a colorimetric readout analyzed with a plate reader.

Sulfo phospho vanillin assay method principle
First, a crude or purified lipid source is applied to a 96 well plate. Then concentrated sulfuric acid is added and the samples are heated to solubilize and prime the total lipid sample, followed by the addition of vanillin in an acid solution. The lipids react with vanillin in the presence of the acids to form a colorimetric product that is easily detected on a microplate reader at 540 nm.

Sulfo phospho vanillin assay protocol summary
- add samples to plate
- incubate for 30-min at 90C
- cool for 5-min at 4C
- transfer samples to clean plate
- read background at 540 nm
- add vanillin reagent and mix
- incubate for 15-min at 37C
- analyse on plate reader

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab242305-100Test", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Vanillin Reagent", "number":"AB242305-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"Purified Lipid Standard", "number":"AB242305-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Gut microbes 17:2489072 PubMed40205678

2025

Creatine-mediated ferroptosis inhibition is involved in the intestinal radioprotection of daytime-restricted feeding.

Applications

Unspecified application

Species

Unspecified reactive species

Yingjuan He,Gaomei Zhao,Xue Ouyang,Shaobo Wang,Yin Chen,Chenwenya Li,Yongwu He,Jining Gao,Songling Han,Jinghong Zhao,Junping Wang,Cheng Wang

Microorganisms 13: PubMed40142444

2025

Mechanisms of Resistance to Cannabidiol.

Applications

Unspecified application

Species

Unspecified reactive species

Iddrisu Ibrahim,Joseph Atia Ayariga,Junhuan Xu,Daniel A Abugri,Robertson K Boakai,Olufemi S Ajayi

Cell death discovery 11:88 PubMed40044676

2025

Targeting Caveolin-1 for enhanced rotator cuff repair: findings from single-cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Shanhong Fang,Songye Wu,Peng Chen

Pathophysiology : the official journal of the International Society for Pathophysiology 32: PubMed39982361

2025

Evolocumab Reduces Oxidative Stress and Lipid Peroxidation in Obese Zucker Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Cebova,Radoslava Bulkova,Olga Pechanova

Materials today. Bio 31:101483 PubMed39896276

2025

Development and in vitro evaluation of biomimetic injectable hydrogels from decellularized human nerves for central nervous system regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Gopal Agarwal,Kennedy Moes,Christine E Schmidt

MedComm 5:e70033 PubMed39669976

2024

Inhibition of Selenoprotein I promotes ferroptosis and reverses resistance to platinum chemotherapy by impairing Akt phosphorylation in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Mimi Chen,Dingwen Huang,Ziyin Li,Yu Chen,Jinhua Huang,Yuanqun Chen,Zhili Zhou,Zhiying Yu

Nature cell biology 26:542-551 PubMed38454050

2024

Loss of WIPI4 in neurodegeneration causes autophagy-independent ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Zhu,Motoki Fujimaki,Louisa Snape,Ana Lopez,Angeleen Fleming,David C Rubinsztein

Frontiers in microbiology 14:1289987 PubMed38045029

2023

Metabolic flux reprogramming in -infected human macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Khushboo Borah Slater,Luana Moraes,Ye Xu,Daniel Kim

International journal of molecular sciences 24: PubMed37958857

2023

Surgical Site-Released Tissue Is Potent to Generate Bone onto TCP and PCL-TCP Scaffolds In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Emely Rehage,Andrea Sowislok,André Busch,Eleftherios Papaeleftheriou,Melissa Jansen,Marcus Jäger

Nature communications 14:2571 PubMed37156797

2023

Lipid flipping in the omega-3 fatty-acid transporter.

Applications

Unspecified application

Species

Unspecified reactive species

Chi Nguyen,Hsiang-Ting Lei,Louis Tung Faat Lai,Marc J Gallenito,Xuelang Mu,Doreen Matthies,Tamir Gonen

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Lipid Assay Kit (unsaturated fatty acids)

Functional Studies - Lipid Assay Kit (unsaturated fatty acids) (AB242305)

Functional Studies - Lipid Assay Kit (unsaturated fatty acids) (AB242305)

Key facts

Detection method

Sample types

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (14)

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