The Lipid Assay Kit ab242305 measures the lipid content (unsaturated fatty acids only) of samples using the sulfo-phospho-vanillin method resulting in a simple colorimetric readout amenable to multi-well plate detection.
Colorimetric
Plasma, Tissue, Cell culture media, Serum
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The Lipid Assay Kit ab242305 measures the lipid content (unsaturated fatty acids only) of samples using the sulfo-phospho-vanillin method resulting in a simple colorimetric readout amenable to multi-well plate detection.
Colorimetric
Plasma, Tissue, Cell culture media, Serum
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The Lipid Assay Kit (unsaturated fatty acids) (ab242305) measures the lipid content (unsaturated fatty acids only) of samples using the sulfo-phospho-vanillin method resulting in a simple colorimetric readout amenable to multi-well plate detection.
First, a crude or purified lipid source is applied to a 96 well plate. Then concentrated sulfuric acid is added and the samples are heated to solubilize and prime the total lipid sample, followed by the addition of vanillin in an acid solution. The lipids react with vanillin in the presence of the acids to form a colorimetric product that is easily detected on a microplate reader.
Each kit provides sufficient reagents to perform 100 assays including standards and unknown samples.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Detection of lipids from Human Serum or Human Plasma.
Fifteen microliters of undiluted Human Serum, Human Plasma, or Negative Control buffer were analyzed using ab242305.
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