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AB238538

Lipid Peroxidation (4-HNE) Assay Kit

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(77 Publications)

Lipid Peroxidation (4-HNE) Assay Kit ab238538 is a competitive assay used to detect and quantify 4-hydroxynonenal (4-HNE)-protein adducts. Readout on any colorimetric (450 nm) plate reader.

- Convenient method to detect 4-HNE adduct as a marker of oxidative damage/ oxidative stress
-Cited in >70 publications
- Complete kit including standard curve for quantitation
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Competitive ELISA - Lipid Peroxidation (4-HNE) Assay Kit (AB238538)
  • cELISA

Supplier Data

Competitive ELISA - Lipid Peroxidation (4-HNE) Assay Kit (AB238538)

HNE-BSA Competitive ELISA Standard Curve.

The following figures demonstrate typical HNE Adduct Competitive ELISA results. One should use the data below for reference only. This data should not be used to interpret actual results.

Key facts

Detection method

Colorimetric

Sample types

Purified protein, Tissue Lysate, Plasma, Serum, Cell Lysate

Assay type

Competitive

Assay Platform

Microplate reader

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Product details

How the assay works

This Lipid Peroxidation (4-HNE) Assay Kit will allow the quantitation of 4-HNE adduct in protein samples determined by comparing its absorbance with that of a known 4-HNE-BSA standard curve.4-HNE is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress.

Lipid Peroxidation (4-HNE) Assay Kit protocol summary:

- Prepare all reagents, samples, and standards as instructed.
- Add 50 µL standard or sample to wells of 4-HNE Conjugate coated plate and incubate for 10 mins. Add 50 µL of the diluted anti-4-HNE antibody and incubate for 1 h.
- Washing steps with 250 µL 1X Wash Buffer.
- Add 100 µL diluted Secondary Antibody-HRP Conjugate per well and incubate for 1 h. Wash as before with 1X Wash buffer.
- Add 100 µL of warm Substrate Solution and incubate for 2-20 mins.
- Stop the enzyme reaction by adding 100 µL of Stop Solution to each well. Read absorbance immediately on a microplate reader using 450 nm.

How other researchers are using

Lipid Peroxidation (4-HNE) Assay Kit has been used in variety of sample type including:
- Mouse osteoclast cells 1
- Rat brain tissues 2
- Human glioma tissues 3

References:
1 - Louka P et al. 2024; 2 - Davaanyam D et at 2023; 3 - Wang Z et al. 2023.

Related and recommended products

See below alternatives Lipid Peroxidation Assay Kits:
- Lipid Peroxidation Assay Kit (Cell-based) ab243377
- Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) ab118970
- Lipid Peroxidation (MDA) Assay Kit (Colorimetric) ab233471

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

4-Hydroxynonenal (4-HNE) often referred to as HNE is a highly reactive aldehyde with a molar mass of approximately 156.19 g/mol. It plays a significant role in lipid peroxidation processes. 4-HNE forms during the oxidative degradation of polyunsaturated fatty acids and is present mostly in tissues with high metabolic activity. It is frequently expressed in the liver brain and heart where oxidative stress is elevated. Researchers commonly use techniques like 4-HNE staining and 4-HNE Western blot to study its presence and implications in various tissues and cells.
Biological function summary

4-HNE serves as a signaling molecule influencing many cellular pathways. It is not just a byproduct of oxidative stress but actively modifies proteins through covalent binding. These modifications often lead to changes in protein function and structure affecting cellular processes such as proliferation apoptosis and differentiation. 4-HNE also interacts with other reactive molecules and antioxidants forming a complex network of cellular responses. This aldehyde regulates gene expression influencing how cells respond to oxidative stress and inflammation.

Pathways

4-HNE interacts with pathways involved in oxidative stress and detoxification. In the oxidative stress pathway it reacts with glutathione to form glutathione conjugates which are part of the cell’s detoxifying responses. The aldehyde thereby affects the Keap1-Nrf2-ARE pathway which modulates antioxidant responses and cellular protection against oxidative damage. Proteins like glutathione peroxidase and glutathione-S-transferase play key roles in these pathways illustrating the interconnectedness of 4-HNE with cellular defense mechanisms.

4-HNE has associations with conditions such as Alzheimer's disease and cardiovascular diseases. In Alzheimer's the aldehyde contributes to neuroinflammation and protein modification impacting neuronal function and survival. It interacts with proteins like tau and amyloid-beta which are critical in the pathology of Alzheimer's. In cardiovascular diseases 4-HNE mediates cellular damage and inflammation linking it to the progression of atherosclerosis. This reactive aldehyde interacts with proteins such as apolipoprotein B highlighting its significant role in disease pathways linked to oxidative stress.
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Product protocols

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Target data

See full target information 4-Hydroxynonenal
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Publications (77)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 57:1391-1405 PubMed40589411

2025

Modulation of ferroptosis via YY1-SLC7A11 axis in hepatic ischemia-reperfusion injury pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shaochuang Wang,Baofei Jiang,Jun Cao,Ting Xu,Chengming Zhou,Xiangyou Yu,Yi Wang,Yu Xie,Lindong Ji,Guangrong Zhou,Hao Wen,Long Ma,Kun Wu

BMC cancer 25:676 PubMed40229746

2025

Polygonum multiflorum Stilbene Glycoside Oligomers induce the ferroptosis of triple negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomeng Lin,Hua Yang,Tingting Cai,Zhangshuo Yang,Siyun Niu,Hui Jia

Journal of nanobiotechnology 23:249 PubMed40133959

2025

Radiation-induced ferroptosis via liposomal delivery of 7-Dehydrocholesterol.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwen Li,Shuyue Zhan,Wei Yang,He Zhang,Xinrui Ma,Fanghui Chen,Amy Li,Pakteema Tong,Fangchao Jiang,Zhengwei Cao,Ian Delahunty,Jiayi Wang,Yufei Wu,Zhi Liu,Zibo Li,Yong Teng,Libin Xu,Jin Xie

Cell communication and signaling : CCS 23:141 PubMed40102920

2025

Increased melanin induces aberrant keratinocyte - melanocyte - basal - fibroblast cell communication and fibrogenesis by inducing iron overload and ferroptosis resistance in keloids.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangguang Shi,Xueyi Xia,Yang Xiao,Ying Zhang,Yiyi Gong,Yahui Chen,Chenyi Shi,Wei Wang,Jianlan Liu,Jia Huang,Mengguo Liu,Zhuoya Xu,Yanyun Ma,Mengkun Shi,Jiucun Wang,Wenyu Wu

Redox biology 81:103572 PubMed40023977

2025

Indoleamine 2,3-dioxygenase 1 drives epithelial cells ferroptosis in influenza-induced acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxin Zheng,Yu Zhang,Yubiao Chen,Xiumei Deng,Baiyun Liu,Qiang Xu,Chuyun Qian,Zhihui Zhang,Ke Wang,Yuan Zeng,Zhenting Liang,Ling Sang,Lingbo Nong,Xiaoqing Liu,Yonghao Xu,Yimin Li,Yongbo Huang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2415814 PubMed40019375

2025

MISP Suppresses Ferroptosis via MST1/2 Kinases to Facilitate YAP Activation in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fuquan Zhang,Bingtao Huang,Yiming Xu,Guohong Cao,Mingjing Shen,Changmin Liu,Judong Luo

International journal of molecular sciences 26: PubMed39941157

2025

SMURF1-Induced Ubiquitination of FTH1 Disrupts Iron Homeostasis and Suppresses Myogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Xiong,Wen Li,Chunlin Yu,Mohan Qiu,Zengrong Zhang,Chenming Hu,Shiliang Zhu,Li Yang,Han Pen,Xiaoyan Song,Jialei Chen,Bo Xia,Shunshun Han,Chaowu Yang

Communications biology 8:159 PubMed39901028

2025

Arginine methyltransferase PRMT1 promotes ferroptosis through EGR1/GLS2 axis in sepsis-related acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Min Li,Longhui Hu,Qiao Ke,Zhao Li,Chujun Ruan,Hanjing Lu,Xiaoran Liu

International journal of biological sciences 21:1259-1274 PubMed39897031

2025

TRIM32 promotes neuronal ferroptosis by enhancing K63-linked ubiquitination and subsequent p62-selective autophagic degradation of GPX4.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhou,Yuqing Zhao,Shixue Huang,Haoming Shu,Yinuo Zhang,Haiyuan Yang,Yilong Ren,Xuhui Zhou,Wei Liu,Tengfei Song,Jianquan Zhao,Jun Ma

Frontiers in endocrinology 15:1412373 PubMed39877845

2025

Relationship between ferroptosis and healing of diabetic foot ulcer: a prospective clinical study.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Jiang,Long Tang,Shuao Xiao
View all publications
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