AB243377

Lipid Peroxidation Assay Kit (Cell-based)

Name: Lipid Peroxidation Assay Kit (Cell-based,Fluorescent) (ab243377) | Abcam
Brand: Abcam Limited
SKU: AB243377

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(28 Publications)

Lipid Peroxidation Assay Kit (Cell-based) (ab243377) uses a sensitive ratiometric Lipid Peroxidation Sensor that changes its fluorescence from red to green upon peroxidation by ROS in cells, this peroxidation-dependent shift enables the ratiometric measurement of lipid peroxidation.

- Includes H2O2 positive control treatment to induce lipid peroxidation
- Compatible with FITC/TRITC or FITC/PE channels
- Cited in over 20 publications

2 Images

Fluorescence Microscopy - Lipid Peroxidation Assay Kit (Cell-based) (AB243377)

Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Lipid Peroxidation Assay Kit (Cell-based) (AB243377)

HeLa cells were stained with 1X Lipid Peroxidation Sensor for 30 minutes in complete growth medium at 37°C.

For H₂O₂ treatment, approximately 250 μM H₂O₂ was added to the cells and incubated for 30 minutes. The cells were then incubated with 1X Lipid peroxidation Sensor, and stained with Hoechst 33342 during the last 10 minutes of incubation. The cells were washed 3 times with HHBS and imaged with a Keyence fluorescent microscope. With H₂O₂ treatment, a clear shift of fluorescence signal of red to green was observed.

Flow Cytometry - Lipid Peroxidation Assay Kit (Cell-based) (AB243377)

Flow Cyt

Supplier Data

Flow Cytometry - Lipid Peroxidation Assay Kit (Cell-based) (AB243377)

Jurkat cells were stained with 1X Lipid Peroxidation Reagent for 30 minuts in complete growth medium at 37°C.

For H₂O₂ treatment, approximately 250 μM H₂O₂ was added to the cells and incubated for 30 minutes. The cells were then incubated with 1X Lipid Peroxidation Sensor, and analyzed with a flow cytometer through FITC (488/530 nm) and PE (488/572 nm) channels. The data are represented as the ratios of red (PE)/green (FITC) fluorescence intensities. The ratio of red/green decreases in H₂O₂ treated cells indicating the presence of H₂O₂-induced lipid peroxidation.

Key facts

Detection method

Fluorescent

Assay Platform

Flow cytometer, Fluorescence microscope

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Product details

Lipid Peroxidation Assay Kit (Cell-based) (ab243377) uses a sensitive ratiometric Lipid Peroxidation Sensor that changes its fluorescence from red to green upon peroxidation by ROS in cells, this peroxidation-dependent shift enables the ratiometric measurement of lipid peroxidation. Our kit includes H₂O₂ as a positive control treatment to induce lipid peroxidation.

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What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab243377-200Test", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"HHBS", "number":"AB243377-CMP01", "productcode":"" }, { "size":"1 x 50 µL", "name":"Ratiometric lipid peroxidation sensor R590/G525 (500X)", "number":"AB243377-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"3% H2O2", "number":"AB243377-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Product protocols

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Target data

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 57:804-819 PubMed40164686

2025

NR4A1 suppresses breast cancer growth by repressing c-Fos-mediated lipid and redox dyshomeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Cen Jiang,Youzhi Zhu,Junsi Zhang,Huaying Chen,Weiwei Li,Ruiwang Xie,Lingjun Kong,Ling Chen,Xiangjin Chen,Huifang Huang,Sunwang Xu

International journal of biological sciences 21:1275-1293 PubMed39897035

2025

Exosomal mediated lipid metabolism, ferritinophagy and CoQ-dependent pathway contributes to the ferroptosis of keratinocyte in SJS/TEN.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhang,Pei Qiao,ChunYing Xiao,ZiPeng Cao,JiaoLing Chen,Hui Fang,JianKang Yang,ZeHua Kang,ErLe Dang,Shuai Shao,BingYu Pang,QingYang Li,ZhenLai Zhu,ShengXian Shen,Akito Hasegawa,Riichiro Abe,HongJiang Qiao,Gang Wang,Meng Fu

BMC biology 23:24 PubMed39849491

2025

Effects of genetic ablation and pharmacological inhibition of HuR on gene expression, iron metabolism, and hormone levels.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalie Idlin,Sivakumar Krishnamoorthy,Magdalena Wolczyk,Mouad Fakhri,Michal Lechowski,Natalia Stec,Jacek Milek,Pratik Kumar Mandal,Jaroslaw Cendrowski,Christos Spanos,Magdalena Dziembowska,Katarzyna Mleczko-Sanecka,Juri Rappsilber,Gracjan Michlewski

Redox biology 75:103255 PubMed39029270

2024

Inhibition of ferroptosis rescues M2 macrophages and alleviates arthritis by suppressing the HMGB1/TLR4/STAT3 axis in M1 macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuan Feng,Feiyang Meng,Fei Huo,Yumeng Zhu,Yifei Qin,Yu Gui,Hai Zhang,Peng Lin,Qian He,Yong Li,Jiejie Geng,Jiao Wu

Antioxidants (Basel, Switzerland) 13: PubMed38790651

2024

Use of Optical Redox Imaging to Quantify Alveolar Macrophage Redox State in Infants: Proof of Concept Experiments in a Murine Model and Human Tracheal Aspirates Samples.

Applications

Unspecified application

Species

Unspecified reactive species

He N Xu,Diego Gonzalves,Jonathan H Hoffman,Joseph A Baur,Lin Z Li,Erik A Jensen

Cell reports. Medicine 5:101476 PubMed38508138

2024

Pyroptotic T cell-derived active IL-16 has a driving function in ovarian endometriosis development.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghe Zhang,Weidong Zhao,Yonggang Zhou,Shengdi Xi,Xiuxiu Xu,Xianghui Du,Xiaohu Zheng,Weiping Hu,Rui Sun,Zhigang Tian,Binqing Fu,Haiming Wei

Cell death discovery 10:9 PubMed38182558

2024

MUC1-C is a target of salinomycin in inducing ferroptosis of cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuaki Daimon,Atrayee Bhattacharya,Keyi Wang,Naoki Haratake,Ayako Nakashoji,Hiroki Ozawa,Yoshihiro Morimoto,Nami Yamashita,Takeo Kosaka,Mototsugu Oya,Donald W Kufe

European archives of psychiatry and clinical neuroscience 274:777-791 PubMed37653354

2023

The atypical antidepressant tianeptine confers neuroprotection against oxygen-glucose deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Burcu Ersoy,Marie-Louise Herzog,Wen Pan,Simone Schilling,Matthias Endres,Ria Göttert,Golo D Kronenberg,Karen Gertz

Cells 12: PubMed37408275

2023

Effect of Photobiomodulation on Protein Kinase Cδ, Cytochrome C, and Mitochondria in U87 MG Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Viktória Pevná,Georges Wagnières,Daniel Jancura,Veronika Huntošová

PLoS pathogens 19:e1010942 PubMed37027441

2023

A compensatory RNase E variation increases Iron Piracy and Virulence in multidrug-resistant Pseudomonas aeruginosa during Macrophage infection.

Applications

Unspecified application

Species

Unspecified reactive species

Mylene Vaillancourt,Anna Clara Milesi Galdino,Sam P Limsuwannarot,Diana Celedonio,Elizabeth Dimitrova,Matthew Broerman,Catherine Bresee,Yohei Doi,Janet S Lee,William C Parks,Peter Jorth

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Lipid Peroxidation Assay Kit (Cell-based)

Fluorescence Microscopy - Lipid Peroxidation Assay Kit (Cell-based) (AB243377)

Flow Cytometry - Lipid Peroxidation Assay Kit (Cell-based) (AB243377)

Key facts

Detection method

Assay Platform

Complementary Products

Secondary Antibodies

Assay Kits

Assay Kits

Primary Antibodies

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (28)

Product promise