Lipid Peroxidation Assay Kit (Cell-based)

2 product images

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  Fluorescence Microscopy - Lipid Peroxidation Assay Kit (Cell-based) (ab243377)

  HeLa cells were stained with 1X Lipid Peroxidation Sensor for 30 minutes in complete growth medium at 37°C.

  For H₂O₂ treatment, approximately 250 μM H₂O₂ was added to the cells and incubated for 30 minutes. The cells were then incubated with 1X Lipid peroxidation Sensor, and stained with Hoechst 33342 during the last 10 minutes of incubation. The cells were washed 3 times with HHBS and imaged with a Keyence fluorescent microscope. With H₂O₂ treatment, a clear shift of fluorescence signal of red to green was observed.

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  Flow Cytometry - Lipid Peroxidation Assay Kit (Cell-based) (ab243377)

  Jurkat cells were stained with 1X Lipid Peroxidation Reagent for 30 minuts in complete growth medium at 37°C.

  For H₂O₂ treatment, approximately 250 μM H₂O₂ was added to the cells and incubated for 30 minutes. The cells were then incubated with 1X Lipid Peroxidation Sensor, and analyzed with a flow cytometer through FITC (488/530 nm) and PE (488/572 nm) channels. The data are represented as the ratios of red (PE)/green (FITC) fluorescence intensities. The ratio of red/green decreases in H₂O₂ treated cells indicating the presence of H₂O₂-induced lipid peroxidation.