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Lipid Peroxidation (MDA) Assay Kit (Colorimetric) ab233471 enables researchers to detect MDA without the heating steps required by the TBARS assay conventionally used for MDA detection.

Lipid Peroxidation (MDA) Assay Kit (Colorimetric) designed for success:
- Very fast and specific for MDA with little interference from other aldehydes
- Simple protocol for standard 96-well plate reader

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Images

Functional Studies - Lipid Peroxidation (MDA) Assay Kit (Colorimetric) (AB233471), expandable thumbnail
  • Functional Studies - Lipid Peroxidation (MDA) Assay Kit (Colorimetric) (AB233471), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Suspension cells, Adherent cells

Reactivity data

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

What's included?

200 Test
Components
Dilution Buffer
1 x 10 mL
MDA Color Reagent
1 x 1 Vial
MDA Standard
1 x 1 Vial
Reaction Solution
1 x 10 mL

Recommended products

Lipid Peroxidation (MDA) Assay Kit (Colorimetric) ab233471 enables researchers to detect MDA without the heating steps required by the TBARS assay conventionally used for MDA detection.

Lipid Peroxidation (MDA) Assay Kit (Colorimetric) designed for success:
- Very fast and specific for MDA with little interference from other aldehydes
- Simple protocol for standard 96-well plate reader

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Suspension cells, Adherent cells

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Lipid Peroxidation (MDA) Assay Kit (Colorimetric) ab233471 enables researchers to detect MDA without the heating steps required by the TBARS assay conventionally used for MDA detection.

In this MDA assay, the MDA Color Reagent reacts with MDA to generate a blue color product which is measured at 695 nm with absorbance microplate readers. The assay is very fast and specific for MDA with little interference from other aldehydes.

Alternatively, see our popular TBARS assay kit for MDA measurement Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) ab118970.

MDA assay protocol summary for ab233471:
- add samples and standards to wells
- add MDA color reagent and incubate for 10-30 min at room temp
- add reaction solution and incubate for 30-60 min at room temp
- analyze with microplate reader

Lipid peroxidation is characterized by the oxidative degradation of unsaturated fatty acids, phospholipids, glycolipids, cholesterol esters and cholesterol. Malondialdehyde (MDA) is one of the most commonly used biomarkers for lipid peroxidation.

Running an MDA assay has historically relied on a reaction with thiobarbituric acid (the TBARS assay) to generate a product that can be measured colorimetrically at 532 nm or fluorimetrically at Ex/Em = 530/550 nm.

However, the TBARS assay has quite a few limitations:
- the reaction is not specific to MDA,
- the TBA-MDA reaction needs be run under acidic conditions,
- the TBARS assay needs be run under high temperature, commonly at 90-100 °C.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Lipid Peroxidation (MDA) Assay Kit (Colorimetric) (ab233471), expandable thumbnail

    Functional Studies - Lipid Peroxidation (MDA) Assay Kit (Colorimetric) (ab233471)

    Signal Comparison of MDA, Formaldehyde, and Glyceraldehyde

  • Functional Studies - Lipid Peroxidation (MDA) Assay Kit (Colorimetric) (ab233471), expandable thumbnail

    Functional Studies - Lipid Peroxidation (MDA) Assay Kit (Colorimetric) (ab233471)

    MDA dose response was measured with ab233471 on a 96-well clear bottom microplate using a SpectraMax microplate reader (Molecular Devices). (Pathcheck on (Left image); Pathcheck off (Right image))

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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