Lipid Peroxidation (MDA) Assay Kit ab118970 uses a 60 minute 95°C incubation, followed by 10 min on ice. Reaction of malondialdehyde (MDA) with TBA produces TBA-MDA with a colorimetric (532 nm) or fluorometric (Ex/Em 532/553 nm) assay readout.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Publications
- Open medicine (Warsaw, Poland) 19:202409452024Mesenchymal stem cell-derived exosomal miR-26a induces ferroptosis, suppresses hepatic stellate cell activation, and ameliorates liver fibrosis by modulating SLC7A11.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Cao,Huan Yang,Yan Huang,Jian Lu,Hong Du,Bingying WangPubMed 38756248
- Current stem cell research & therapy 19:725-7342024Use of Mesenchymal Stem Cells in Experimental Ovarian Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanan Fouad,Ibrahim A Albahlol,Hazim A Wahab,Eman H Nadwa,Heba M Galal,Mohamed Abouelkheir,Ahmed E Taha,Abdelkarim G Kamel,Hassan A AbdelmawllaPubMed 37448361
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Plasma, Cell culture extracts, Tissue Extracts, Urine (UTI)
- Assay type
Quantitative
- Assay time
1h 20m
- Sensitivity
> 0.1 nmol/well
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Lipid Peroxidation (MDA) Assay Kit ab118970 uses a 60 minute 95°C incubation, followed by 10 min on ice. Reaction of malondialdehyde (MDA) with TBA produces TBA-MDA with a colorimetric (532 nm) or fluorometric (Ex/Em 532/553 nm) assay readout.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
Colorimetric/Fluorometric
- Sample types
Plasma, Cell culture extracts, Tissue Extracts, Urine (UTI)
- Assay type
Quantitative
- Assay time
1h 20m
- Assay Platform
Microplate reader
- Sensitivity
> 0.1 nmol/well
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970) provides a convenient tool for sensitive detection of malondialdehyde (MDA).
In the lipid peroxidation assay protocol, the MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
The MDA assay is also refered to as a TBARS assay.
Lipid peroxidation assay protocol summary:
- add TBA solution to samples and standards, incubate at 95°C for 60 min, cool in ice bath for 10 min
- transfer to wells of microplate
- analyze with microplate reader
For higher sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet before analysis.
For an alternative MDA assay, without the heating steps required in the TBARS assay, try MDA assay Lipid Peroxidation (MDA) Assay Kit (Colorimetric) ab233471.
Technical Note: MDA is unlikely to be detectable in healthy urine but may be elevated in the presence of infection or disease of the urinary tract.
Lipid peroxidation refers to the oxidative degradation of lipids. In this process free radicals take electrons from the lipids (generally in cell membranes), resulting in cell damage. Quantification of lipid peroxidation is essential to assess oxidative stress. Lipid peroxidation forms reactive aldehydes such as malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as natural bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are often used as markers of lipid peroxidation, and to assay for oxidative damage / oxidative stress. **Related products** Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader. Also see the popular as an alternative marker of lipid peroxidation and oxidative stress. **How other researchers have used Lipid Peroxidation Assay Kit ab118970** The MDA/TBARs assay kit has been used in publications in a variety of sample types, including: - Human: serum1, hippocampal primary cell extracts2, A375 cultured cell lysates3, plasma and platelet samples4 - Mouse: neuronal cell lysates5, heart tissue extract6, plasma7, cell extracts8 - Rat: hippocampal tissue extracts9, cardiomyocyte extracts of cultured cells10, lung lysates11 - Pig: serum12 References: 1 - Shen J et al. 2018, 2 - Wang Q et al. 2019, 3 - Luo M et al. 2018, 4 - Mustafa AG et al. 2018, 5 - Murphy K et al. 2018, 6 - Guan F et al. 2019, 7 - Costa CRC et al. 2018, 8 - Eleftheriadis T et al. 2019, 9 - Malekiyan et al. 2019, 10 - Zhou Z et al. 2018, 11 - Li L et al. 2018, 12 - Lee SE and Kang KS 2019
Supplementary info
- Activity summary
Lipid peroxidation is a biochemical process that involves the oxidative degradation of lipids which are essential components of cellular membranes. It is often referred to by scientists as lipid peroxidation or simply peroxidation. This process mainly occurs in polyunsaturated fatty acids and plays a significant role in cell membrane damage. Lipid peroxidation leads to the formation of reactive aldehydes including malondialdehyde (MDA) which serve as an important marker for oxidative stress detectable by mda assay kits. In tissues lipid peroxidation occurs prominently in cells with high oxidative metabolism such as the liver brain and muscle tissues.
- Biological function summary
This process results in structural and functional impairment of cell membranes potentially affecting cellular functions. Lipid peroxidation does not operate as part of a larger complex but generates secondary products such as MDA which can form adducts with proteins and DNA thereby disrupting cellular integrity. Scientists frequently measure these products using specialized tools like the malondialdehyde test and lipid peroxidation assay as these provide insights into the extent of oxidative damage within biological samples such as blood tissues and organs.
- Pathways
Lipid peroxidation is critically involved in the oxidative stress response and inflammation pathways. The oxidative stress response pathway highlights the imbalance between antioxidants and reactive oxygen species (ROS) leading to damage. Antioxidant proteins such as glutathione peroxidase and superoxide dismutase can counteract peroxidation. The inflammation pathway connects lipid peroxidation to cellular mechanisms that trigger inflammatory signals involving proteins like cyclooxygenase and lipoxygenase.
- Associated diseases and disorders
Lipid peroxidation significantly contributes to the pathophysiology of conditions such as Alzheimer's disease and atherosclerosis. In Alzheimer's oxidative stress and resulting lipid peroxidation damage neural cells linking the process to proteins such as amyloid-beta. In atherosclerosis the peroxidation of low-density lipoproteins (LDL) in vascular walls initiates plaque formation and connects to proteins like apolipoprotein B. Researchers utilize MDA test kits to assess the extent of lipid peroxidation offering insights into disease progression and potential therapeutic targets.
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5 product images
Image courtesy of Hichor et al. Sci Rep. 2018; 8: 2524. doi: 10.1038/s41598-018-20980-3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.Functional Studies - Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970)
Lipid Peroxidation Assay performed on mouse sciatic nerve samples. Hichor M et al. used the TBARS assay / MDA assay ab118970 to study the role of LXRs in the regulation of oxidative stress in peripheral nerves.
They identified that in sciatic nerves in LXR knockout mice (LXRdKO), the MDA concentration was significantly increased, and that this was corrected by the treatment of mice with the anti-oxidant ROS scavenger N-acetylcysteine (NAC).
Image courtesy of Ravarotto V et al. PLoS One. 2018; 13(9): e0204618. doi: 10.1371/journal.pone.0204618. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.Functional Studies - Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970)
Lipid Peroxidation measured with MDA assay in Fabry patients and healthy controls.
Ravarotto V et al. used Lipid Peroxidation Assay Kit ab118970 to assess oxidative stress in Fabry disease. They identified that MDA levels are higher in Fabry patients, indicating higher levels of oxidative stress.
The MDA concentration was measured in plasma from Fabry patients compared to healthy control patients. Data are shown ±SEM. *: p = 0.01.
Functional Studies - Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970)
Measurement of MDA in human plasma (20 μl) and rat liver lysate (10 mg).
Functional Studies - Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970)
MDA assay standard curve.
Typical MDA standard calibration curve using colorimetric reading.
Functional Studies - Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970)
MDA assay standard curve.
Typical MDA standard calibration curve using fluorometric reading.
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