Lipolysis Assay Kit (Colorimetric) ab185433 provides a simple and robust way to measure measure the amount of glycerol released from cells during lipolysis. Lipolysis Assay Kit (Colorimetric) designed for success:
- Simple and fast protocol with results in less than one hour
- Highly sensitive of which the detection limit can be as low as 0.2 nmol/well.
- Suitable for high-throughput screeningIndividual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Revista brasileira de farmacognosia : orgao oficial da Sociedade Brasileira de Farmacognosia 33:334-3432023Quercetin-3--rutinoside from Downregulates Adipogenesis and Lipid Accumulation and Improves Glucose Uptake by Activation of AMPK/Glut-4 in 3T3-L1 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuni Swamy Ganjayi,Reddy Sankaran Karunakaran,Sreedevi Gandham,Balaji MerigaPubMed 36819090
- Clinical and translational medicine 12:e11082022Myoglobin-mediated lipid shuttling increases adrenergic activation of brown and white adipocyte metabolism and is as a marker of thermogenic adipocytes in humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLisa Christen,Helen Broghammer,Inka Rapöhn,Kevin Möhlis,Christian Strehlau,Aleix Ribas-Latre,Claudia Gebhardt,Lisa Roth,Kerstin Krause,Kathrin Landgraf,Antje Körner,Kerstin Rohde-Zimmermann,Anne Hoffmann,Nora Klöting,Adhideb Ghosh,Wenfei Sun,Hua Dong,Christian Wolfrum,Tienush Rassaf,Ulrike B Hendgen-Cotta,Michael Stumvoll,Matthias Blüher,John T Heiker,Juliane WeinerPubMed 36480426
- Metabolites 11:2021Anti-Obesity Effect of Hot Water Extract of Barley Sprout through the Inhibition of Adipocyte Differentiation and Growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMyeong-Jin Kim,Hye-Won Kawk,Sang-Hyeon Kim,Hyo-Jae Lee,Ji-Won Seo,Jong-Tae Kim,Seung-Hee Jang,Min-Jeong Kim,Young-Min KimPubMed 34564426
- Frontiers in endocrinology 12:6986212021Laminin-α4 Is Upregulated in Both Human and Murine Models of Obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Goddi,Alanis Carmona,Liesl Schroedl,Jeremy M White,Matthew J Piron,Avelino De Leon,Isabel Casimiro,Alexandria Hoffman,Maria A Gonzalez Porras,Eric M Brey,Matthew J Brady,Ronald N CohenPubMed 34394003
- Frontiers in pharmacology 12:7040742021Anti-Obesity and Lipid Lowering Activity of Bauhiniastatin-1 is Mediated Through PPAR-γ/AMPK Expressions in Diet-Induced Obese Rat Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesReddy Sankaran Karunakaran,Oruganti Lokanatha,Ganjayi Muni Swamy,Chintha Venkataramaiah,Muppuru Muni Kesavulu,Chippada Appa Rao,Kameswara Rao Badri,Meriga BalajiPubMed 34366856
- Cells 10:2021SIRT5 Inhibition Induces Brown Fat-Like Phenotype in 3T3-L1 Preadipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancesca Molinari,Alessandra Feraco,Simone Mirabilii,Serena Saladini,Luigi Sansone,Enza Vernucci,Giada Tomaselli,Vincenzo Marzolla,Dante Rotili,Matteo A Russo,Maria Rosaria Ricciardi,Agostino Tafuri,Antonello Mai,Massimiliano Caprio,Marco Tafani,Andrea ArmaniPubMed 34066961
- 3 Biotech 11:2332021A bioactive fraction of inhibits adipogenesis and inflammation in 3T3-L1 cells via modulation of PPAR-γ/SREBP-1c and TNF-α/IL-6.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarunakaran Reddy Sankaran,Muni Swamy Ganjayi,Lokanatha Oruganti,Appa Rao Chippada,Balaji MerigaPubMed 33968577
- Adipocyte 10:216-2312021Cleavage of the vaspin N-terminus releases cell-penetrating peptides that affect early stages of adipogenesis and inhibit lipolysis in mature adipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCatherine A Tindall,Estelle Erkner,Jan Stichel,Annette G Beck-Sickinger,Anne Hoffmann,Juliane Weiner,John T HeikerPubMed 33866927
- The Biochemical journal 477:2735-27542020MAPK-interacting kinase 2 (MNK2) regulates adipocyte metabolism independently of its catalytic activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames E Merrett,Jianling Xie,Peter J Psaltis,Christopher G ProudPubMed 32648926
- Arteriosclerosis, thrombosis, and vascular biology 40:61-712019CD73 Promotes Age-Dependent Accretion of Atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNadia R Sutton,Diane Bouïs,Kris M Mann,Imran M Rashid,Alexandra L McCubbrey,Matt C Hyman,Daniel R Goldstein,Annie Mei,David J PinskyPubMed 31619062
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant
- Assay type
- Quantitative
- Reactive species
- Mammals
- Range
- 2 - 10 nmol/well
- Assay time
- 40m
- Sensitivity
- < 0.2 nmol/well
What's included?
Recommended products
Lipolysis Assay Kit (Colorimetric) ab185433 provides a simple and robust way to measure measure the amount of glycerol released from cells during lipolysis. Lipolysis Assay Kit (Colorimetric) designed for success:
- Simple and fast protocol with results in less than one hour
- Highly sensitive of which the detection limit can be as low as 0.2 nmol/well.
- Suitable for high-throughput screeningIndividual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant
- Assay type
- Quantitative
- Reactive species
- Mammals
- Range
- 2 - 10 nmol/well
- Assay time
- 40m
- Assay Platform
- Microplate reader
- Sensitivity
- < 0.2 nmol/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
How the assay works
In this Lipolysis Assay Kit protocol, glycerol kinase phosphorylates glycerol released into the medium; glycerol phosphate oxidase oxidizes glycerol-1-phosphate to dihydroxyacetone phosphate and hydrogen peroxide.
A quinoneimine dye is produced when 4-aminoantipyrine and sodium N-ethytl-N-(3-sulfopropyl)m-anisidine couple with hydrogen peroxide.
The absorbance of the dye is measured at 570nm.
The increase in absorbance is proportional to the amount of glycerol in the sample.
Lipolysis assay protocol summary:
- Add samples and standards to wells
- Add reaction mix
- Incubate for 30 min
- Analyze with microplate reader
Related and recommended products
For fluorometric detection, we recommend Lipolysis Assay Kit (Fluorometric) Lipolysis Assay Kit (Fluorometric) ab185434
This product is manufactured by BioVision, an Abcam company and was previously called K577 Lipolysis (3T3-L1) Colorimetric Assay Kit. K577-100 is the same size as the 100 test size of ab185433.
Lipolysis is the hydrolysis of triglycerides within the cell into glycerol and free fatty acids. The glycerol and free fatty acids are then released into the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown adipose tissue. Deficiencies in lipolysis lead to increased intracellular lipid accumulation, resulting in abnormal cellular physiology, hyperlipidemia, and insulin resistance. Lipolysis can be induced by catecholamine and certain hormones. The kit includes synthetic catecholamine, Isoproterenol, which activates ß-adrenergic receptors. This leads to activation of adenylate cyclase, which catalyzes the conversion of ATP to cAMP. cAMP then serves as a second messenger to activate hormone-sensitive lipase, which hydrolyzes the triglycerides. This pathway can be inhibited by insulin.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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2 product images
Functional Studies - Lipolysis Assay Kit (Colorimetric) (ab185433)
Measurement of glycerol level in media of 3T3-L1 cells.
50 μl of 3T3-L1 cells treated with vehicle control (H2O) or 100 nM Isoproterenol for 0-3 hours.
Functional Studies - Lipolysis Assay Kit (Colorimetric) (ab185433)
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