Lipoprotein Lipase Assay Kit (Fluorometric) ab204721 is a simple and accurate assay for measuring Lipoprotein Lipase (LPL) activity.Lipoprotein Lipase Assay Kit designed for success:
- Can be used with a variety of sample types, including cell lysates, tissue extracts, body fluids
- Simple protocol that can be performed in a convenient 96-well
- Fast results in less than one hourIndividual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Nature communications 14:26562023Resveratrol intervention attenuates chylomicron secretion via repressing intestinal FXR-induced expression of scavenger receptor SR-B1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan Pang,Fitore Raka,Alya Abbas Heirali,Weijuan Shao,Dinghui Liu,Jianqiu Gu,Jia Nuo Feng,Chieko Mineo,Philip W Shaul,Xiaoxian Qian,Bryan Coburn,Khosrow Adeli,Wenhua Ling,Tianru JinPubMed 37160898
- British journal of pharmacology 180:1803-18182023The heparan sulfate mimetic Muparfostat aggravates steatohepatitis in obese mice due to its binding affinity to lipoprotein lipase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Zhang,Kai Li,Hao-Ran Sun,Shao-Kun Sun,Ya-Ting Zhu,Yu-Ting Ge,Yu-Xuan Wu,Qin-Yao Zhou,Guan-Ting Li,Xiao-Ai Chang,Peng Sun,Ying Ding,Xiao HanPubMed 36735592
- Frontiers in immunology 13:9994702022CRISPR/Cas9 based blockade of IL-10 signaling impairs lipid and tissue homeostasis to accelerate atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaozhe Shi,Jiabao Guo,Qiongyang Yu,Xinlin Hou,Lili Liu,Mingming Gao,Lili Wei,Ling Zhang,Wei Huang,Yuhui Wang,George Liu,Peter Tontonoz,Xunde XianPubMed 36110841
- Biomedicines 9:2021Adipocyte-Specific ACKR3 Regulates Lipid Levels in Adipose Tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSelin Gencer,Yvonne Döring,Yvonne Jansen,Soyolmaa Bayasgalan,Olga Schengel,Madeleine Müller,Linsey J F Peters,Christian Weber,Emiel P C van der VorstPubMed 33917642
- Molecular metabolism 49:1011972021Adipocyte integrin-linked kinase plays a key role in the development of diet-induced adipose insulin resistance in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAimée R Bugler-Lamb,Annie Hasib,Xiong Weng,Chandani K Hennayake,Chenshi Lin,Rory J McCrimmon,Roland H Stimson,Michael L J Ashford,David H Wasserman,Li KangPubMed 33647469
- The Journal of pharmacology and experimental therapeutics 376:428-4352021RNA Interference Targeting Liver Angiopoietin-Like Protein 3 Protects from Nephrotic Syndrome in a Rat Model Via Amelioration of Pathologic Hypertriglyceridemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYitong Zhao,Masaki Goto,Nosratola D Vaziri,Mahyar Khazaeli,Han Liu,Nazli Farahanchi,Elham Khanifar,Ted Farzaneh,Patrick A Haslett,Hamid Moradi,Mangala M SoundarapandianPubMed 33443084
- Cell 183:1848-1866.e262020Obesity Shapes Metabolism in the Tumor Microenvironment to Suppress Anti-Tumor Immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlison E Ringel,Jefte M Drijvers,Gregory J Baker,Alessia Catozzi,Juan C García-Cañaveras,Brandon M Gassaway,Brian C Miller,Vikram R Juneja,Thao H Nguyen,Shakchhi Joshi,Cong-Hui Yao,Haejin Yoon,Peter T Sage,Martin W LaFleur,Justin D Trombley,Connor A Jacobson,Zoltan Maliga,Steven P Gygi,Peter K Sorger,Joshua D Rabinowitz,Arlene H Sharpe,Marcia C HaigisPubMed 33301708
- Nature communications 11:58472020Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to PPARA agonism and fasting.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChad N Brocker et. al.PubMed 33203882
- Metabolism: clinical and experimental 109:1542962020ApoC2 deficiency elicits severe hypertriglyceridemia and spontaneous atherosclerosis: A rodent model rescued from neonatal death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingming Gao et. al.PubMed 32562799
- MethodsX 7:1008652020Simple and rapid real-time monitoring of LPL activity .Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefan Kluge et. al.PubMed 32274337
Key facts
- Detection method
- Fluorescent
- Sample types
- Purified protein, Tissue Lysate, Plasma, Cell Lysate
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 20m
Target data
Function
Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed:11342582, PubMed:27578112, PubMed:8675619). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (PubMed:12032167, PubMed:7592706). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed:24726386). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed:11342582, PubMed:27811232).
Alternative names
LIPD, LPL, Lipoprotein lipase, Phospholipase A1
What's included?
Recommended products
Lipoprotein Lipase Assay Kit (Fluorometric) ab204721 is a simple and accurate assay for measuring Lipoprotein Lipase (LPL) activity.Lipoprotein Lipase Assay Kit designed for success:
- Can be used with a variety of sample types, including cell lysates, tissue extracts, body fluids
- Simple protocol that can be performed in a convenient 96-well
- Fast results in less than one hourIndividual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Fluorescent
- Sample types
- Purified protein, Tissue Lysate, Plasma, Cell Lysate
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 20m
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Please refer to protocols
Notes
How the assay works
Lipoprotein Lipase Assay Kit (Fluorometric) contains a quenched substrate that fluoresces upon hydrolysis by lipoprotein lipase (LPL). The fluorometric intensity is directly proportional to the amount of substrate hydrolyzed.
This lipase assay detects total lipase activity when no inhibitor is used. Comparing results in the presence or absence of an LPL inhibitor allows for quantification of LPL activity specifically.
Our results indicate that the majority (~90%) of lipase activity detected by this kit in post-heparin treated mouse plasma is from LPL. To determine the exact LPL specific activity in mouse plasma, measure activity in pre- and post-heparin treated plasma.
Lipoprotein Lipase Assay Kit summary:
- Add samples and standards to wells
- Add reaction mix
- Pre-incubate the plate at 37°C for 10 minutes
- Measure fluorescence (Ex/Em= 482/515 nm) in a kinetic mode
for 1 hour at 37°C
How other researchers are using
Lipoprotein Lipase Assay Kit (Fluorometric) has been used in a variety of sample type including:
- Human Caco-2 cells 1
- Mouse Adipose tissue homogenate 2
- Mouse spleen samples 3
References:
1-Pang G al. 2022
2-Gencer et al. 2021
3-Ringel A et al. 2020
This product is manufactured by BioVision, an Abcam company and was previously called K721 Lipoprotein Lipase Activity Fluorometric Assay Kit. K721-100 is the same size as the 100 test size of ab204721.
Lipoprotein lipase (LPL) is a member of the lipase family that hydrolyzes triglycerides in chylomicrons and very low-density lipoprotein (VLDL). Digestion of triglycerides in VLDL by LPL leads to their conversion to intermediate-density lipoprotein (IDL) and then low-density lipoprotein (LDL). LPL is found attached to the luminal surface of endothelial cells in the heart, muscle, and adipose tissue. Mutations in lipoprotein lipase can lead to a variety of disorders such as lipoprotein metabolism, hypertriglyceridemia etc. Overexpression of LPL in mice has been shown to promote obesity and insulin resistance.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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4 product images
Functional Studies - Lipoprotein Lipase Assay Kit (Fluorometric) (ab204721)
Typical LPL Substrate Standard Curve.
Functional Studies - Lipoprotein Lipase Assay Kit (Fluorometric) (ab204721)
Measurement of LPL activity in purified enzyme from Pseudomonas sp. (5 ng), post-heparin treated mouse plasma (2 μl), 7-day post-differentiated 3T3-L1 cell lysate (100 μg), and rat heart lysate (200 μg)
Functional Studies - Lipoprotein Lipase Assay Kit (Fluorometric) (ab204721)
Inhibition of LPL activity from post-heparin treated mouse plasma by Angptl 4, an LPL specific inhibitor. The assay was run for 1 hour and the activity was determined by calculating the slope. The IC50 was determined to be 22.6 nM.
Functional Studies - Lipoprotein Lipase Assay Kit (Fluorometric) (ab204721)
Inhibition of Positive Control by Orlistat, a generic lipase inhibitor. The assay was run for 1 hour and the IC50 was determined to be 11.4 μM.
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Product protocols
- Visit the general protocols
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