AB115347

Live and Dead Cell Assay

Name: Live and dead cell assay. Fluorescent. Cell viability. ab115347 | Abcam
Brand: Abcam Limited
SKU: AB115347

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(54 Publications)

Live Dead Assay Kit ab115347 is a one-step assay that differentially labels live and dead cells with fluorescent dyes suitable for fluorescence microscopy or flow cytometry.

- Add dyes directly into cell culture media
- Live cell dye labels intact, viable cells in green: Ex/Em: 494/515 nm
- Dead cell dye labels in red: Ex/Em: 528/617 nm
- Cited in over 50 publications

4 Images

Functional Studies - Live and Dead Cell Assay (AB115347)

FuncS

Supplier Data

Functional Studies - Live and Dead Cell Assay (AB115347)

Jurkat cells stained with the live/dead assay kit. Jurkat cells treated with a drug to induce cell death were labeled with the live/dead assay stain. Live cells (with esterase activity) stain green and dead cells (compromised plasma membrane) stain red. (A) Field of cells following 10 minute staining in media of live/dead stain. (B)Magnified view showing that in live cells the whole cell is stained green whereas in dead red cells it is the fragmented nuclear DNA that is stained.

FuncS

Supplier Data

Functional Studies - Live and Dead Cell Assay (AB115347)

Dot plots showing live/dead analysis of vehicle or drug treated Jurkat cells (day 3 of treatment). The indicated drug is used to induce cell death. Live cells are on the y-axis and dead cells are on the x-axis. The red polygongate identifies live cells and the number indicates the percent of live cells.

FuncS

Supplier Data

Functional Studies - Live and Dead Cell Assay (AB115347)

The sample dot plots demonstrate varying ratios of live and dead cells. More green = upper left = live cells; more red = lower right = dead cells.

FuncS

Unknown

Functional Studies - Live and Dead Cell Assay (AB115347)

Quantification of % viable cells of Jurkat cells treated with a dose response of the inidicated drug (to induce cell death) and analyzed using the live/dead assay stain on days 1, 2 and 3 using flow cytometry.

Key facts

Detection method

Fluorescent

Assay Platform

Flow cytometer, Fluorescence microscope

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Product details

Live Dead Assay Kit ab115347 differentially labels live and dead cells with fluorescent dyes with a one-step live dead assay protocol. It is used for the rapid quantitation of cell viability using flow cytometry or fluorescent microscopy.

The Live Dead assay staining solution is a mixture of two fluorescent dyes that differentially label live and dead cells.

The Live cell dye labels intact, viable cells green. It is membrane permeant and non-fluorescent until ubiquitous intracellular esterases remove ester groups and render the molecule fluorescent. The Excitation (max) and Emission(max) are 494nm and 515nm (similar to FITC).

The Dead cell dye labels cells with compromised plasma membranes red. It is membrane-impermeant and binds to DNA with high affinity. Once bound to DNA, the fluorescence increases >30-fold. The Excitation (max) and Emission(max) are 528nm and 617nm.

The Live Dead assay protocol uses a one-step staining procedure that is simple and fast. It can be used directly in cell culture media.

This assay is not suitable for use with fixed cells / cell fixation. The Live Dead assay staining solution provided is sufficient for ~1000 assays. **Related assays** Review the to learn about kits to perform a , and .

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What's included?

{ "values": { "1000Test": { "sellingSize": "1000 Test", "publicAssetCode":"ab115347-1000Test", "assetComponentDetails": [ { "size":"1 x 0.1 mL", "name":"1000X Live/Dead Cell stain in DMSO", "number":"AB115347-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

See full target information Calcein AM

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Publications (54)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 22:e13966 PubMed37803909

2023

In need of age-appropriate cardiac models: Impact of cell age on extracellular matrix therapy outcomes.

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S Gulberk Ozcebe,Pinar Zorlutuna

International journal of nanomedicine 18:2019-2035 PubMed37155503

2023

Fabrication of Interleukin-4 Encapsulated Bioactive Microdroplets for Regulating Inflammation and Promoting Osteogenesis.

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Yi Zhang,Jin Cao,Minghui Jian,Zhixiao Zhou,Nadia Anwar,Lan Xiao,Yaping Ma,Dingmei Zhang,Jun Zhang,Xin Wang

Macromolecular rapid communications 44:e2300025 PubMed36794543

2023

"Out-of-the-box" Granular Gel Bath Based on Cationic Polyvinyl Alcohol Microgels for Embedded Extrusion Printing.

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Jinfeng Zeng,Zhengtian Xie,Yasumasa Dekishima,Setsuka Kuwagaki,Norihito Sakai,Michiya Matsusaki

International journal of bioprinting 9:678 PubMed37274002

2023

Monolayer heterojunction interactive hydrogels for high-freedom 4D shape reconfiguration by two-photon polymerization.

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Yufeng Tao,Chengchangfeng Lu,Xuejiao Wang,Zhiduo Xin,Xia Cao,Yunpeng Ren

Journal of orthopaedic translation 38:141-155 PubMed36381245

2022

Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an and osteoarthritis model.

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Yu-Huan Hsueh,Waradee Buddhakosai,Phung Ngan Le,Yung-Yi Tu,Hsien-Chang Huang,Huai-En Lu,Wen-Liang Chen,Yuan-Kun Tu

Frontiers in bioengineering and biotechnology 10:995460 PubMed36263353

2022

A tunable physiomimetic stretch system evaluated with precision cut lung slices and recellularized human lung scaffolds.

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Oskar Rosmark,Arturo Ibáñez-Fonseca,Johan Thorsson,Göran Dellgren,Oskar Hallgren,Anna-Karin Larsson Callerfelt,Linda Elowsson,Gunilla Westergren-Thorsson

Nature communications 13:3385 PubMed35697674

2022

High throughput, label-free isolation of circulating tumor cell clusters in meshed microwells.

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Mert Boya,Tevhide Ozkaya-Ahmadov,Brandi E Swain,Chia-Heng Chu,Norh Asmare,Ozgun Civelekoglu,Ruxiu Liu,Dohwan Lee,Sherry Tobia,Shweta Biliya,L DeEtte McDonald,Bassel Nazha,Omer Kucuk,Martin G Sanda,Benedict B Benigno,Carlos S Moreno,Mehmet A Bilen,John F McDonald,A Fatih Sarioglu

ACS sensors 7:1747-1756 PubMed35671439

2022

Combination of Live Cell Surface-Enhanced Raman Scattering Imaging with Chemometrics to Study Intracellular Nanoparticle Dynamics.

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Elisa Lenzi,Malou Henriksen-Lacey,Beatriz Molina,Judith Langer,Carlos D L de Albuquerque,Dorleta Jimenez de Aberasturi,Luis M Liz-Marzán

Bioengineered 13:12955-12971 PubMed35615975

2022

Senescence marker protein30 protects lens epithelial cells against oxidative damage by restoring mitochondrial function.

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He Teng,Yaru Hong,Jingjing Cao,Hui Li,Fang Tian,Jing Sun,Kai Wen,Guoge Han,Amy Whelchel,Xiaomin Zhang,Xiaorong Li,Lijie Dong

International journal of biological macromolecules 212:370-380 PubMed35613678

2022

Anionic exopolysaccharide from Cryptococcus laurentii 70766 as an alternative for alginate for biomedical hydrogels.

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Species

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Masoud Hamidi,Hafez Jafari,Julia Siminska-Stanny,Oseweuba Valentine Okoro,Ahmed Fatimi,Amin Shavandi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Live and Dead Cell Assay

Functional Studies - Live and Dead Cell Assay (AB115347)

Functional Studies - Live and Dead Cell Assay (AB115347)

Functional Studies - Live and Dead Cell Assay (AB115347)

Functional Studies - Live and Dead Cell Assay (AB115347)

Key facts

Detection method

Assay Platform

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (54)

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