Live Dead Assay Kit ab115347 is a one-step assay that differentially labels live and dead cells with fluorescent dyes suitable for fluorescence microscopy or flow cytometry.
- Add dyes directly into cell culture media
- Live cell dye labels intact, viable cells in green: Ex/Em: 494/515 nm
- Dead cell dye labels in red: Ex/Em: 528/617 nm
- Cited in over 50 publications
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- Aging cell 22:e139662023In need of age-appropriate cardiac models: Impact of cell age on extracellular matrix therapy outcomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Gulberk Ozcebe,Pinar ZorlutunaPubMed 37803909
- International journal of nanomedicine 18:2019-20352023Fabrication of Interleukin-4 Encapsulated Bioactive Microdroplets for Regulating Inflammation and Promoting Osteogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Zhang,Jin Cao,Minghui Jian,Zhixiao Zhou,Nadia Anwar,Lan Xiao,Yaping Ma,Dingmei Zhang,Jun Zhang,Xin WangPubMed 37155503
- Macromolecular rapid communications 44:e23000252023"Out-of-the-box" Granular Gel Bath Based on Cationic Polyvinyl Alcohol Microgels for Embedded Extrusion Printing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinfeng Zeng,Zhengtian Xie,Yasumasa Dekishima,Setsuka Kuwagaki,Norihito Sakai,Michiya MatsusakiPubMed 36794543
- International journal of bioprinting 9:6782023Monolayer heterojunction interactive hydrogels for high-freedom 4D shape reconfiguration by two-photon polymerization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYufeng Tao,Chengchangfeng Lu,Xuejiao Wang,Zhiduo Xin,Xia Cao,Yunpeng RenPubMed 37274002
- Journal of orthopaedic translation 38:141-1552022Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an and osteoarthritis model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Huan Hsueh,Waradee Buddhakosai,Phung Ngan Le,Yung-Yi Tu,Hsien-Chang Huang,Huai-En Lu,Wen-Liang Chen,Yuan-Kun TuPubMed 36381245
- Frontiers in bioengineering and biotechnology 10:9954602022A tunable physiomimetic stretch system evaluated with precision cut lung slices and recellularized human lung scaffolds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOskar Rosmark,Arturo Ibáñez-Fonseca,Johan Thorsson,Göran Dellgren,Oskar Hallgren,Anna-Karin Larsson Callerfelt,Linda Elowsson,Gunilla Westergren-ThorssonPubMed 36263353
- Nature communications 13:33852022High throughput, label-free isolation of circulating tumor cell clusters in meshed microwells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMert Boya,Tevhide Ozkaya-Ahmadov,Brandi E Swain,Chia-Heng Chu,Norh Asmare,Ozgun Civelekoglu,Ruxiu Liu,Dohwan Lee,Sherry Tobia,Shweta Biliya,L DeEtte McDonald,Bassel Nazha,Omer Kucuk,Martin G Sanda,Benedict B Benigno,Carlos S Moreno,Mehmet A Bilen,John F McDonald,A Fatih SariogluPubMed 35697674
- ACS sensors 7:1747-17562022Combination of Live Cell Surface-Enhanced Raman Scattering Imaging with Chemometrics to Study Intracellular Nanoparticle Dynamics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisa Lenzi,Malou Henriksen-Lacey,Beatriz Molina,Judith Langer,Carlos D L de Albuquerque,Dorleta Jimenez de Aberasturi,Luis M Liz-MarzánPubMed 35671439
- Bioengineered 13:12955-129712022Senescence marker protein30 protects lens epithelial cells against oxidative damage by restoring mitochondrial function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHe Teng,Yaru Hong,Jingjing Cao,Hui Li,Fang Tian,Jing Sun,Kai Wen,Guoge Han,Amy Whelchel,Xiaomin Zhang,Xiaorong Li,Lijie DongPubMed 35615975
- International journal of biological macromolecules 212:370-3802022Anionic exopolysaccharide from Cryptococcus laurentii 70766 as an alternative for alginate for biomedical hydrogels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasoud Hamidi,Hafez Jafari,Julia Siminska-Stanny,Oseweuba Valentine Okoro,Ahmed Fatimi,Amin ShavandiPubMed 35613678
Key facts
- Detection method
- Fluorescent
Target data
What's included?
Recommended products
Live Dead Assay Kit ab115347 is a one-step assay that differentially labels live and dead cells with fluorescent dyes suitable for fluorescence microscopy or flow cytometry.
- Add dyes directly into cell culture media
- Live cell dye labels intact, viable cells in green: Ex/Em: 494/515 nm
- Dead cell dye labels in red: Ex/Em: 528/617 nm
- Cited in over 50 publications
Key facts
- Detection method
- Fluorescent
- Assay Platform
- Flow cytometer, Fluorescence microscope
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Live Dead Assay Kit ab115347 differentially labels live and dead cells with fluorescent dyes with a one-step live dead assay protocol. It is used for the rapid quantitation of cell viability using flow cytometry or fluorescent microscopy.
The Live Dead assay staining solution is a mixture of two fluorescent dyes that differentially label live and dead cells.
The Live cell dye labels intact, viable cells green. It is membrane permeant and non-fluorescent until ubiquitous intracellular esterases remove ester groups and render the molecule fluorescent. The Excitation (max) and Emission(max) are 494nm and 515nm (similar to FITC).
The Dead cell dye labels cells with compromised plasma membranes red. It is membrane-impermeant and binds to DNA with high affinity. Once bound to DNA, the fluorescence increases >30-fold. The Excitation (max) and Emission(max) are 528nm and 617nm.
The Live Dead assay protocol uses a one-step staining procedure that is simple and fast. It can be used directly in cell culture media.
This assay is not suitable for use with fixed cells / cell fixation. The Live Dead assay staining solution provided is sufficient for ~1000 assays. **Related assays** Review the to learn about kits to perform a , and .
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4 product images
Functional Studies - Live and Dead Cell Assay (ab115347)
Dot plots showing live/dead analysis of vehicle or drug treated Jurkat cells (day 3 of treatment). The indicated drug is used to induce cell death. Live cells are on the y-axis and dead cells are on the x-axis. The red polygongate identifies live cells and the number indicates the percent of live cells.
Functional Studies - Live and Dead Cell Assay (ab115347)
Quantification of % viable cells of Jurkat cells treated with a dose response of the inidicated drug (to induce cell death) and analyzed using the live/dead assay stain on days 1, 2 and 3 using flow cytometry.
Functional Studies - Live and Dead Cell Assay (ab115347)
The sample dot plots demonstrate varying ratios of live and dead cells. More green = upper left = live cells; more red = lower right = dead cells.
Functional Studies - Live and Dead Cell Assay (ab115347)
Jurkat cells stained with the live/dead assay kit. Jurkat cells treated with a drug to induce cell death were labeled with the live/dead assay stain. Live cells (with esterase activity) stain green and dead cells (compromised plasma membrane) stain red. (A) Field of cells following 10 minute staining in media of live/dead stain. (B)Magnified view showing that in live cells the whole cell is stained green whereas in dead red cells it is the fragmented nuclear DNA that is stained.
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