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Live Dead Assay Kit ab115347 differentially labels live and dead cells with fluorescent dyes with a one-step live dead assay protocol.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

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Images

Key facts

Detection method

Fluorescent

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What's included?

1000 Test
Components
1000X Live/Dead Cell stain in DMSO
1 x 0.1 mL

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Live Dead Assay Kit ab115347 differentially labels live and dead cells with fluorescent dyes with a one-step live dead assay protocol.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Fluorescent

Assay Platform

Flow cytometer, Fluorescence microscope

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Live Dead Assay Kit ab115347 differentially labels live and dead cells with fluorescent dyes with a one-step live dead assay protocol. It is used for the rapid quantitation of cell viability using flow cytometry or fluorescent microscopy.

The Live Dead assay staining solution is a mixture of two fluorescent dyes that differentially label live and dead cells.

The Live cell dye labels intact, viable cells green. It is membrane permeant and non-fluorescent until ubiquitous intracellular esterases remove ester groups and render the molecule fluorescent. The Excitation (max) and Emission(max) are 494nm and 515nm (similar to FITC).

The Dead cell dye labels cells with compromised plasma membranes red. It is membrane-impermeant and binds to DNA with high affinity. Once bound to DNA, the fluorescence increases >30-fold. The Excitation (max) and Emission(max) are 528nm and 617nm.

The Live Dead assay protocol uses a one-step staining procedure that is simple and fast. It can be used directly in cell culture media.

This assay is not suitable for use with fixed cells / cell fixation. The Live Dead assay staining solution provided is sufficient for ~1000 assays. **Related assays** Review the to learn about kits to perform a , and .

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Functional Studies - Live and Dead Cell Assay (ab115347), expandable thumbnail

    Functional Studies - Live and Dead Cell Assay (ab115347)

    Dot plots showing live/dead analysis of vehicle or drug treated Jurkat cells (day 3 of treatment). The indicated drug is used to induce cell death. Live cells are on the y-axis and dead cells are on the x-axis. The red polygongate identifies live cells and the number indicates the percent of live cells.

  • Functional Studies - Live and Dead Cell Assay (ab115347), expandable thumbnail

    Functional Studies - Live and Dead Cell Assay (ab115347)

    Quantification of % viable cells of Jurkat cells treated with a dose response of the inidicated drug (to induce cell death) and analyzed using the live/dead assay stain on days 1, 2 and 3 using flow cytometry.

  • Functional Studies - Live and Dead Cell Assay (ab115347), expandable thumbnail

    Functional Studies - Live and Dead Cell Assay (ab115347)

    The sample dot plots demonstrate varying ratios of live and dead cells. More green = upper left = live cells; more red = lower right = dead cells.

  • Functional Studies - Live and Dead Cell Assay (ab115347), expandable thumbnail

    Functional Studies - Live and Dead Cell Assay (ab115347)

    Jurkat cells stained with the live/dead assay kit. Jurkat cells treated with a drug to induce cell death were labeled with the live/dead assay stain. Live cells (with esterase activity) stain green and dead cells (compromised plasma membrane) stain red. (A) Field of cells following 10 minute staining in media of live/dead stain. (B)Magnified view showing that in live cells the whole cell is stained green whereas in dead red cells it is the fragmented nuclear DNA that is stained.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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