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AB65470

Live and Dead Cell Staining Kit

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(13 Publications)

Live-Dead Cell Staining Kit ab65470 provides the ready-to-use reagents for convenient discrimination between live and dead cells.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Schematic Diagram - Live and Dead Cell Staining Kit (AB65470)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Live and Dead Cell Staining Kit (AB65470)

Representative image of Live and Dead Cell Staining Kit ab65470

Components shown from left to right :

- Solution A

- Solution B

- Binding Buffer II

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Direct

Sensitivity

= 75 pg/mL

Range

0.156 - 10 ng/mL

Assay time

20m

Assay Platform

Microplate reader, Fluorescence microscope

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Fluorescence Microscopy": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This Live-Dead Cell Staining Kit provides the ready-to-use reagents for convenient discrimination between live and dead cells. The kit utilizes a cell-permeable green fluorescent dye (Ex/Em = 488/518 nm), to stain live cells. Dead cells can be easily stained by propidium iodide (PI), a cell non-permeable red fluorescent dye (Ex/Em = 536/615). Stained live and dead cells can be visualized by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine). The kit provides sufficient reagents for 100 stainings using 24-well plate.

Visit our FAQs page for tips and troubleshooting.

Distinguishing between live and dead cells is very important for investigation of growth control and cell death.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab65470-96Test", "assetComponentDetails": [ { "size":"1 x 50 µL", "name":"Solution B", "number":"AB65470-CMP03", "productcode":"" }, { "size":"1 x 50 µL", "name":"Solution A", "number":"AB65470-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"Binding Buffer II", "number":"AB65470-CMP01", "productcode":"AB323957" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Please refer to protocols
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Product protocols

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Target data

See full target information Propidium iodide
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 20:6399-6421 PubMed40416730

2025

Anti-Infection Efficacy, Osteogenesis Potential, and Biocompatibility of 3D Printed PLGA/Nano-Hydroxyapatite Porous Scaffolds Grafted with Vancomycin/DOPA/rhBMP-2 in Infected Rabbit Bone Defects.

Applications

Unspecified application

Species

Unspecified reactive species

A Li Mu Ke Re Mu,Maimaitiaili Abulikemu,Zhilin Liang,Abudurusuli Abulikemu,Aikebaier Tuxun

International journal of stem cells : PubMed36042011

2022

Synergistic Effect of Hydrogen and 5-Aza on Myogenic Differentiation through the p38 MAPK Signaling Pathway in Adipose-Derived Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyong Fei,Erkai Pang,Lei Hou,Jihang Dai,Mingsheng Liu,Xuanqi Wang,Bin Xie,Jingcheng Wang

Molecular cancer 21:45 PubMed35148751

2022

Engineered exosomes as an in situ DC-primed vaccine to boost antitumor immunity in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lanxiang Huang,Yuan Rong,Xuan Tang,Kezhen Yi,Peng Qi,Jinxuan Hou,Weihuang Liu,Yuan He,Xing Gao,Chunhui Yuan,Fubing Wang

Stem cells translational medicine 10:303-319 PubMed33049125

2020

Tissue-specific mesenchymal stem cell-dependent osteogenesis in highly porous chitosan-based bone analogs.

Applications

Unspecified application

Species

Unspecified reactive species

Swati Midha,Krishan G Jain,Nitu Bhaskar,Amtoj Kaur,Sonali Rawat,Shibashish Giri,Bikramjit Basu,Sujata Mohanty

Drug design, development and therapy 14:2657-2665 PubMed32764870

2020

-Acetyl-l-Leucine-Polyethyleneimine-Mediated Delivery of CpG Oligodeoxynucleotides 2006 Inhibits RAW264.7 Cell Osteoclastogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Huining Wang,Wenwen Yu,Hongyan Li,Yi Zheng,Zhen Chen,Hongbing Lin,Yuqin Shen

RSC advances 10:14885-14891 PubMed35497169

2020

CpG oligodeoxynucleotides inhibit the proliferation and osteoclastic differentiation of RAW264.7 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zheng,Wenwen Yu,Hongyan Li,Hongbing Lin,Zhen Chen,Huishan Chen,Peipei Zhang,Yue Tian,Xiaowei Xu,Yuqin Shen

Frontiers in cellular neuroscience 13:590 PubMed32116554

2020

Blebbistatin Inhibits Neomycin-Induced Apoptosis in Hair Cell-Like HEI-OC-1 Cells and in Cochlear Hair Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Song Gao,Cheng Cheng,Maohua Wang,Pei Jiang,Liyan Zhang,Ya Wang,Huihui Wu,Xuanfu Zeng,Hui Wang,Xia Gao,Yongming Ma,Renjie Chai

Scientific reports 9:9050 PubMed31227775

2019

Boiling Histotripsy-induced Partial Mechanical Ablation Modulates Tumour Microenvironment by Promoting Immunogenic Cell Death of Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Ki Joo Pahk,Cheol-Hee Shin,In Yeong Bae,Yoosoo Yang,Sang-Heon Kim,Kisoo Pahk,Hyungmin Kim,Seung Ja Oh

Genes to cells : devoted to molecular & cellular mechanisms 21:1380-1386 PubMed27862696

2016

Small spheroids of adipose-derived stem cells with time-dependent enhancement of IL-8 and VEGF-A secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichi Furuhata,Yuka Kikuchi,Shunsuke Tomita,Keitaro Yoshimoto

Autophagy 12:1738-1758 PubMed27463126

2016

Absence of autophagy promotes apoptosis by modulating the ROS-dependent RLR signaling pathway in classical swine fever virus-infected cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Pei,Jieru Deng,Zuodong Ye,Jiaying Wang,Hongchao Gou,Wenjun Liu,Mingqiu Zhao,Ming Liao,Lin Yi,Jinding Chen
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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