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AB187966

Live Cell Labeling Kit - Blue Fluorescence (Ex405nm) - Cytopainter

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(1 Publication)

Live Cell Labeling Kit - Blue Fluorescence (Ex405nm) | Cytopainter (ab187966) is designed to uniformly label live cells in green fluorescence for the studies that require the fluorescent tag molecules retained inside cells for a relatively longer time.
1 Images
Immunocytochemistry/ Immunofluorescence - Live Cell Labeling Kit - Blue Fluorescence (Ex405nm) - Cytopainter (AB187966)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Live Cell Labeling Kit - Blue Fluorescence (Ex405nm) - Cytopainter (AB187966)

HeLa cells with CytoPainter Live Cell Labeling Kit - Blue Fluorescence (Ex405nm).

Image of Hela cells in a black wall/clear bottom 96-well plate using CytoPainter Live Cell Labeling Kit - Blue Fluorescence (Ex405nm)

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Assay Platform

Flow cytometer, Fluorescence microscope

Product details

Live Cell Labeling Kit - Blue Fluorescence (Ex405nm) | Cytopainter (ab187966) is designed to uniformly label live cells in green fluorescence for the studies that require the fluorescent tag molecules retained inside cells for a relatively longer time. The kit uses a non-fluorescent dye that carries a cell-retaining moiety. The dye becomes strongly fluorescent upon entering into live cells, and is trapped inside cells to give stable fluorescence signal that can be measured at Ex/Em = 410/450 nm when excited with a violet laser at Ex=405 nm. The dye is a hydrophobic compound that easily permeates intact live cells.

The labeling process is robust, requiring minimal hands-on time. This product can be readily adapted for many different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol, and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).

Abcam's CytoPainter Cell Labeling Kits are a set of tools used to label cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced healthcare materials 13:e2302268 PubMed37748773

2023

Ex Vivo Evaluation of Combination Immunotherapy Using Tumor-Microenvironment-on-Chip.

Applications

Unspecified application

Species

Unspecified reactive species

Hsuan-Yu Mu,Chiao-Min Lin,Li-An Chu,Ya-Hui Lin,Ji Li,Chao-Yu Liu,Hsi-Chien Huang,Sheng-Liang Cheng,Tsung-Ying Lee,Hsin Mei Lee,Hsin-Min Chen,Yun-Jen Tsai,Yunching Chen,Jen-Huang Huang
View all publications

Product promise

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