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Live Cell Labeling Kit - Blue Fluorescence (Ex405nm) | Cytopainter (ab187966) is designed to uniformly label live cells in green fluorescence for the studies that require the fluorescent tag molecules retained inside cells for a relatively longer time.

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Images

Immunocytochemistry/ Immunofluorescence - Live Cell Labeling Kit - Blue Fluorescence (Ex405nm) - Cytopainter (AB187966), expandable thumbnail

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Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells
Assay type
Cell-based
Reactive species
Mammals

Associated Products

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What's included?

200 Test
Components
HHBS
1 x 100 mL
Labeling Dye Blue (Ex405nm)
2 x 1 Vial

Recommended products

Live Cell Labeling Kit - Blue Fluorescence (Ex405nm) | Cytopainter (ab187966) is designed to uniformly label live cells in green fluorescence for the studies that require the fluorescent tag molecules retained inside cells for a relatively longer time.

Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells
Assay type
Cell-based
Reactive species
Mammals
Assay Platform
Flow cytometer, Fluorescence microscope

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Live Cell Labeling Kit - Blue Fluorescence (Ex405nm) | Cytopainter (ab187966) is designed to uniformly label live cells in green fluorescence for the studies that require the fluorescent tag molecules retained inside cells for a relatively longer time. The kit uses a non-fluorescent dye that carries a cell-retaining moiety. The dye becomes strongly fluorescent upon entering into live cells, and is trapped inside cells to give stable fluorescence signal that can be measured at Ex/Em = 410/450 nm when excited with a violet laser at Ex=405 nm. The dye is a hydrophobic compound that easily permeates intact live cells.

The labeling process is robust, requiring minimal hands-on time. This product can be readily adapted for many different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol, and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).

Abcam's CytoPainter Cell Labeling Kits are a set of tools used to label cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunocytochemistry/ Immunofluorescence - Live Cell Labeling Kit - Blue Fluorescence (Ex405nm) - Cytopainter (ab187966), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Live Cell Labeling Kit - Blue Fluorescence (Ex405nm) - Cytopainter (ab187966)

    HeLa cells with CytoPainter Live Cell Labeling Kit - Blue Fluorescence (Ex405nm).

    Image of Hela cells in a black wall/clear bottom 96-well plate using CytoPainter Live Cell Labeling Kit - Blue Fluorescence (Ex405nm)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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