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AB187967

Live Cell Staining Kit - Green Fluorescence (Ex/Em = 405/525 nm)

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(1 Publication)

Live Cell Staining Kit - Green Fluorescence (Ex/Em = 405/525 nm) (ab187967) is designed to uniformly label live cells in green fluorescence for the studies that require the fluorescent tag molecules retained inside cells for a relatively longer time.
1 Images
Immunocytochemistry/ Immunofluorescence - Live Cell Staining Kit - Green Fluorescence (Ex/Em = 405/525 nm) (AB187967)
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Supplier Data

Immunocytochemistry/ Immunofluorescence - Live Cell Staining Kit - Green Fluorescence (Ex/Em = 405/525 nm) (AB187967)

HeLa cells with CytoPainter Live Cell Labeling Kit - Green Fluorescence

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Assay Platform

Flow cytometer, Fluorescence microscope

Product details

Live Cell Staining Kit - Green Fluorescence (Ex/Em = 405/525 nm) (ab187967) is designed to uniformly label live cells in green fluorescence for the studies that require the fluorescent tag molecules retained inside cells for a relatively longer time. The kit uses a non-fluorescent dye that carries a cell-retaining moiety. The dye becomes strongly fluorescent upon entering into live cells, and is trapped inside cells to give stable fluorescence signal that can be measured at Ex/Em = 410/500 when excited with a violet laser at Ex=405 nm. The dye is a hydrophobic compound that easily permeates intact live cells.

The labeling process is robust, requiring minimal hands-on time. This product can be readily adapted for many different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol, and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).

Abcam's Live Cell Staining Kits are a set of tools used to label cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Society of Nephrology : JA 31:2083-2096 PubMed32675303

2020

Kidney Disease-Associated Variants Have Dose-Dependent, Dominant Toxic Gain-of-Function.

Applications

Unspecified application

Species

Unspecified reactive species

Somenath Datta,Rama Kataria,Jia-Yue Zhang,Savannah Moore,Kaitlyn Petitpas,Adam Mohamed,Nathan Zahler,Martin R Pollak,Opeyemi A Olabisi
View all publications
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