Live/Dead Cell Viability Assay Kit ab287858 provides an easy to use method that is based on the simultaneous determination of live and dead cells using two different fluorescent dyes.
Images
Key facts
- Detection method
- Flow cytometry-fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mammals
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
Application Fluorescence Microscopy | Reactivity Reacts | Dilution info - | Notes - |
Target data
What's included?
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Live/Dead Cell Viability Assay Kit ab287858 provides an easy to use method that is based on the simultaneous determination of live and dead cells using two different fluorescent dyes.
Key facts
- Detection method
- Flow cytometry-fluorescent
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mammals
- Assay Platform
- Flow cytometer, Fluorescence microscope
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
This Live/Dead Cell Viability Assay Kit, provides a two-color fluorescence method that is based on the simultaneous determination of live and dead cells using two different dyes. Live cell dye easily penetrates intact, live cells and intracellular esterase hydrolyzes the dye to produce a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm which can be measured at Ex/Em = 485/530 nm. Dead cell dye enters damaged cell membranes and undergoes a 40-fold enhancement of fluorescence upon binding to nucleic acid, thereby producing a bright red fluorescence (Ex/Em = 495/635 nm) in dead cells. This assay kit provides an easy-to-use, non-radioactive, histological and FACS-based method for measuring cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.
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2 product images
Fluorescence Microscopy - Live/Dead Cell Viability Assay Kit (ab287858)
Live/Dead Cell Viability Assay Kit.
Analysis of Live/Dead HeLa Cells by Microscopy: HeLa cells were cultured overnight with (d-f) or without (a-c) comptothecin (5 μM), which induces apoptosis. Next day, cells were treated with Staining Solution, as described in the protocol. Light and fluorescence images of cells were taken using Nikon TiE microscope. Treatment with comptothecin caused increased apoptosis in cells, which is demonstrated by increased number of dead cells, as shown in f.
Flow Cytometry - Live/Dead Cell Viability Assay Kit (ab287858)
Live/Dead Cell Viability Assay Kit.
Jurkat cells (106 cells/ml) were grown in RPMI media supplemented with 10% FBS. Cells were treated with or without camptothecin (5 μM) overnight. Next day, cells were stained with Staining Solution, as described in the protocol. The graph (right side) displays the cytotoxic effect of the compound, illustrating apoptosis using Dead (Red) and Live (Green) Cell Staining Dye. In control cells (No Treatment), majority of the cells were sorted in FL-1 Channel (Green) and few were in FL-3 (Red) channel. In treated cells, majority of the cells were sorted in FL-3 Channel (Red) and some cells were in FL-1 (Green) channel.
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