Luciferase Reporter Assay Substrate Kit - Firefly (ab228530) provides a fast, simple, and homogeneous bioluminescence assay for the quantification of luciferase activity in live cells and cell extracts.
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- Computational and mathematical methods in medicine 2022:31103302022miR-29b Regulates Lung Cancer Progression by Downregulating FEM1B and Inhibiting the FOX01/AKT Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuanrong Zhang,Rong Wang,Qiuhua DengPubMed 36003920
- Frontiers in cardiovascular medicine 9:6950042022Cardioprotective Effect of Paeonol on Chronic Heart Failure Induced by Doxorubicin Regulating the miR-21-5p/S-Phase Kinase-Associated Protein 2 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCong Chen,Shuhong Liu,Gaozhen Cao,Yang Hu,Run Wang,Min Wu,Mingya Liu,Kai Hang YiuPubMed 35865382
- Molecular medicine reports 26:2022Role of TRIM22 in ulcerative colitis and its underlying mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Ye,Zhongkai LuPubMed 35674157
- RNA biology 19:548-5592022LINC00857 regulated by ZNF460 enhances the expression of CLDN12 by sponging miR-150-5p and recruiting SRSF1 for alternative splicing to promote epithelial-mesenchymal transformation of pancreatic adenocarcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Zhang,Yuan Fang,Lijie Ma,Jing Xu,Chentao Lv,Li Deng,Guanghui ZhuPubMed 35442145
- PeerJ 10:e132012022Comprehensive analysis of the correlations of S100B with hypoxia response and immune infiltration in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Yan,Ya Jun Huang,Qing Yu Huang,Peng Xia Liu,Chang Shan WangPubMed 35368338
- Cell cycle (Georgetown, Tex.) 21:1316-13342022CircCDR1 sponges miR-1290 to regulate cell proliferation, migration, invasion, and apoptosis in esophageal squamous cell cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Fang,Jun Yin,Yaxing Shen,Hao Wang,Han Tang,Xiaosang ChenPubMed 35289243
- Bioengineered 13:6409-64192022Long noncoding RNA endogenous bornavirus-like nucleoprotein acts as an oncogene by regulating microRNA-655-3p expression in T-cell acute lymphoblastic leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhua Yang,Yingying YangPubMed 35220878
- Cell cycle (Georgetown, Tex.) 21:1058-10762022Ultrasound-targeted microbubble destruction-mediated miR-144-5p overexpression enhances the anti-tumor effect of paclitaxel on thyroid carcinoma by targeting STON2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuefeng Chen,Xinyuan Zhang,Yangyang Qian,Enhui Xia,Yu Wang,Qi ZhouPubMed 35184686
- Molecular carcinogenesis 61:494-5072022Transcriptional activation of S100A2 expression by HIF-1α via binding to the hypomethylated hypoxia response elements in HCC cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Yan,Ya Jun Huang,Qing Yu Huang,Peng Xia Liu,Chang Shan WangPubMed 35107180
- International journal of stem cells 14:434-4462021MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/-Catenin Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiong Li,Lei HuangPubMed 34456188
Key facts
- Detection method
- Luminescent
- Sample types
- Cell culture extracts, Cell culture media, Adherent cells
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Target data
Function
Produces green light with a wavelength of 562 nm.
Alternative names
Luciferin 4-monooxygenase, Luciferase
What's included?
Recommended products
Luciferase Reporter Assay Substrate Kit - Firefly (ab228530) provides a fast, simple, and homogeneous bioluminescence assay for the quantification of luciferase activity in live cells and cell extracts.
Key facts
- Detection method
- Luminescent
- Sample types
- Cell culture extracts, Cell culture media, Adherent cells
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Luciferase Reporter Assay Substrate Kit - Firefly (ab228530) provides a fast, simple, and homogeneous bioluminescence assay for the quantification of luciferase activity in live cells and cell extracts. This assay is based on firefly luciferase, a monomeric 61 kD enzyme that catalyses a two-step oxidation of luciferin, which yields light at 560 nm. The assay can be performed in a convenient 96-well and 384-well microtiter-plate format. The signal with a half-life of two to four hours provides a consistent signal across large batches of plates. The assay is compatible with the use of standard cell growth media. It has high sensitivity, and can be used for the assays that require low detection limit.
Common reporter genes include ß-galactosidase, ß-glucuronidase and luciferase. The advantages of a luciferase assay include: high sensitivity, absence of luciferase activity inside most of the cell types, wide dynamic range, speed and low cost. The most versatile and common reporter gene is the luciferase of the North American firefly Photinus pyralis. The protein requires no posttranslational modification for enzyme activity. It is not even toxic in high concentration (in vivo) and can be used in pro- and eukaryotic cells. The firefly luciferase catalyzes the bioluminescent oxidation of luciferin in the presence of ATP, magnesium and oxygen.
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2 product images
Functional Studies - Luciferase Reporter Assay Substrate Kit - Firefly (ab228530)
Luciferase titration curve.
Luciferase dose response was measured with Luciferase Reporter Assay Substrate Kit - Firefly (ab228530) in a white 96-well plate. The kit can detect as low as 0.1 pg/well luciferase with 20 minutes to 5 hours incubation without losing signal intensity. The integration time was 1 second. The half life is more than 4 hours.
Functional Studies - Luciferase Reporter Assay Substrate Kit - Firefly (ab228530)
Reaction Kinetics of CHO-V2R-Luc cells.
Reaction Kinetics of CHO-V2R-Luc cells using Luciferase Reporter Assay Substrate Kit - Firefly (ab228530). CHO cells stably transfected with pCRE-luciferase gene and human Vasopressin receptor 2 (V2R) were plated into a white wall/clear bottom 384-well plate at 15,000 cells/well/25 μL. Cells then were treated with 100 nM of vasopressin in a 37 °C, 5% CO2 incubator for 4 hours. 25 μL of luciferase assay solution was added into the well. The kinetic data was taken every 30 minutes for up to 3 hours. The vasopressin induced luciferase signal is stable for more than 3 hours.
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