Luciferase Reporter Assay Substrate Kit - Firefly

Product details

Luciferase Reporter Assay Substrate Kit - Firefly (ab228530) provides a fast, simple, and homogeneous bioluminescence assay for the quantification of luciferase activity in live cells and cell extracts. This assay is based on firefly luciferase, a monomeric 61 kD enzyme that catalyses a two-step oxidation of luciferin, which yields light at 560 nm. The assay can be performed in a convenient 96-well and 384-well microtiter-plate format. The signal with a half-life of two to four hours provides a consistent signal across large batches of plates. The assay is compatible with the use of standard cell growth media. It has high sensitivity, and can be used for the assays that require low detection limit.

Common reporter genes include ß-galactosidase, ß-glucuronidase and luciferase. The advantages of a luciferase assay include: high sensitivity, absence of luciferase activity inside most of the cell types, wide dynamic range, speed and low cost. The most versatile and common reporter gene is the luciferase of the North American firefly Photinus pyralis. The protein requires no posttranslational modification for enzyme activity. It is not even toxic in high concentration (in vivo) and can be used in pro- and eukaryotic cells. The firefly luciferase catalyzes the bioluminescent oxidation of luciferin in the presence of ATP, magnesium and oxygen.