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Luminescent ATP Detection Assay Kit (ab113849) is used to measure the level of ATP within the cell.

Detection method

Luminescent

Sample types

Suspension cells, Adherent cells

Assay type

Quantitative

Assay time

30m

Images

What's included?

300 Test
Components
Detergent
1 x 20 mL
Lyophilized ATP standard
1 x 1 Vial
Lyophilized substrate
3 x 1 Vial
Substrate Buffer
1 x 20 mL

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Luminescent ATP Detection Assay Kit (ab113849) is used to measure the level of ATP within the cell.

Key facts

Detection method

Luminescent

Sample types

Suspension cells, Adherent cells

Assay type

Quantitative

Assay time

30m

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Luminescent ATP Detection Assay Kit (ab113849) is used to measure the level of ATP within the cell. The luminescent ATP assay protocol involves lysis of the cell sample, addition of luciferase enzyme and luciferin, and measurement of the emitted light using a tube or microplate-based luminometer.

This kit irreversibly inactivates ATP degrading enzymes (ATPases) during the lysis step, ensuring that the luminescent signal obtained truly corresponds to the endogenous levels of ATP.

Luminescent ATP assay protocol summary:
- add ATP standard into standard wells and media into control wells in same plate containing cells to be analyzed
- add detergent solution and incubate for 5 min to lyse cells and stabilize ATP
- add substrate solution and incubate for 5 min
- store plate in dark for 10 min
- analyze on luminescence plate reader

Special Handling Instructions for the ATP Detection Assay Kit

ATP can be found in cells and microbiota on many surfaces. To prevent unintended background, it is recommended to clean bench surfaces and all pipettes to be used during the experiment with 10% bleach. Use of gloves first cleaned by either using 70% ethanol or by changing them frequently is recommended. Use tips and containers that are clean and sterile, such as ATP and nuclease-free consumables. Do not leave reagents or the plate opened while working or during assay incubation

Total levels of cellular ATP can be used to assess cell viability, cell proliferation and cytotoxicity of a wide range of compounds and biological response modifiers. We also offer a very popular alternative colorimetric/fluorometric based on the phosphorylation of glycerol. **Related assays** Review the to learn about kits to perform a , and . Review the to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

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