AB150675

Luxol Fast Blue Stain Kit (Myelin Stain)

Name: Luxol Fast Blue Stain Kit. Myelin Stain. Axonal marker. (ab150675) | Abcam
Brand: Abcam Limited
SKU: AB150675
Price: 170 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(38 Publications)

Luxol fast blue stain kit ab150675 is used for staining myelin/myelinated axons and Nissl bodies in formalin-fixed, paraffin-embedded tissue, as well as frozen tissue.

- Used to identify basic neuronal structure in brain or spinal cord sections
- Simple and fast protocol with ready-to-use solutions
- Cited in over 20 publications

View Alternative Names

Myelin basic protein, MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein, Myelin-oligodendrocyte glycoprotein, MOG

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Luxol Fast Blue Stain Kit (Myelin Stain) (AB150675)

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Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Luxol Fast Blue Stain Kit (Myelin Stain) (AB150675)

Histology - Luxol Fast Blue.

ab150675 (Luxol Fast Blue stain) staining myelin (blue) in formalin fixed paraffin embedded normal human cerebellum, counterstained with cresyl echt violet (purple).

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Luxol Fast Blue Stain Kit (Myelin Stain) (AB150675)

ab150675 Luxol Fast Blue Stain Kit (Myelin Stain) staining formalin-fixed-paraffin embedded mouse brain.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Luxol Fast Blue Stain Kit (Myelin Stain) (AB150675)

Staining using ab150675 - Luxol Fast Blue Stain Kit.

Key facts

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Product details

Luxol Fast Blue Stain Kit (Myelin Stain) ab150675 is designed for staining myelin/myelinated axons and Nissl bodies on formalin fixed, paraffin-embedded tissue as well as frozen tissue. This product is used for identifying the basic neuronal structure in brain or spinal cord sections.

Luxol Fast Blue staining protocol summary:
- deparaffinze sections if necessary and hydrate in water
- incubate in Luxol Fast Blue solution for 24 hr at room temp, or 2 hr at 60°C
- rinse in water
- differentiate by dipping in lithium carbonate solution
- differentiate further by dipping in alcohol reagent
- rinse in water
- incubate in Cresyl Echt Violet for 2-5 min
- rinse in water
- dehydrate in absolute alcohol
- clear and mount

Other products for staining tissue sections

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Staining Interpretation

Myelinated Fibers	Blue
Nissil Substance	Violet
Nerve Cells	Violet

Control Tissue: Cerebral Cortex, Spinal Cord.

Other Notes

Please note, the 125 mL selling size, was previously described as 100 Test. There has been no change to the physical product.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myelin is a lipid-rich substance that insulates nerve fibers in both the central nervous system (CNS) and peripheral nervous system (PNS). It is mainly composed of protein and lipids contributing to its unique insulative properties. Proteolipid protein (PLP) in the CNS and myelin protein zero (MPZ) in the PNS serve as major structural components. Oligodendrocytes in the CNS and Schwann cells in the PNS specifically express this sheath around axons. Myelin does not have a specific mass as it describes the structure rather than a single protein or molecule. Its role in speeding up electrical signals along neurons is recognized by various staining techniques such as Luxol Fast Blue and myelin staining which are helpful in visualizing its structure.

Biological function summary

Myelin plays an important role in the nervous system by facilitating rapid signal transmission between neurons. Myelin enables saltatory conduction where impulses jump from one Node of Ranvier to the next significantly increasing signal speed and efficiency. Its contribution to the formation of the myelin sheath involves interactions with other proteins forming a complex and stable structure. For instance proteins like myelin basic protein (MBP) and PLP integrate into the myelin membrane to ensure compact and stable layering.

Pathways

Myelination integrates closely with the development and function of nerve axons. The myelination process involves the Akt/mTOR signaling pathway which promotes myelin growth and sustains myelin sheath maintenance. Other pathways such as the Nrg1/ErbB pathway regulate Schwann cell function and influence axon myelination. Key proteins related through these pathways include ErbB receptors which interact with neuregulin-1 to influence myelin formation and maintenance.

Myelin degradation significantly impacts multiple sclerosis (MS) and Charcot-Marie-Tooth disease (CMT). In MS demyelination results from an autoimmune response against myelin leading to neurological dysfunctions. Disorders like CMT involve mutations in genes coding for myelin proteins such as PMP22 causing peripheral nerve damage. Both of these conditions highlight the essential connection between myelin and proper nervous system operation with potential disorders disrupting signaling and leading to a range of neurological symptoms.

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Product protocols

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Target data

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

See full target information MBP

Additional targets

MOG

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Publications (38)

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Nature 643:1087-1096 PubMed40369076

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Luxol Fast Blue Stain Kit (Myelin Stain)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Luxol Fast Blue Stain Kit (Myelin Stain) (AB150675)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Luxol Fast Blue Stain Kit (Myelin Stain) (AB150675)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Luxol Fast Blue Stain Kit (Myelin Stain) (AB150675)

Key facts

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Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (38)

Product promise