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AB253380

LysoLive™ Lysosomal Acid Lipase Assay Kit

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(5 Publications)

This kit uses the sensitive substrate LipaGreen to monitor acid lipase activity in the lysosomes of live cells by flow cytometry.

View Alternative Names

Lysosomal acid lipase/cholesteryl ester hydrolase, Acid cholesteryl ester hydrolase, LAL, Cholesteryl esterase, Diacylglycerol lipase, Lipase A, Sterol esterase, Triacylglycerol ester hydrolase, Triacylglycerol lipase, LIPA

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Cellular Activation - LysoLive™ Lysosomal Acid Lipase Assay Kit (AB253380)
  • CellAct

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Cellular Activation - LysoLive™ Lysosomal Acid Lipase Assay Kit (AB253380)

Labeling of healthy cells with LysoLive Lysosomal Assay Kit after treatment with Lipase inhibiter (Orlistat)

Cellular Activation - LysoLive™ Lysosomal Acid Lipase Assay Kit (AB253380)
  • CellAct

Unknown

Cellular Activation - LysoLive™ Lysosomal Acid Lipase Assay Kit (AB253380)

Labeling cells with LysoLive Lysosomal Acid Lipase Assay Kit in healthy cells and those from a LALD patient

Key facts

Detection method

Fluorescent

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Product details

This kit utilizes the sensitive substrate LipaGreen to monitor acid lipase activity in the lysosomes of live cells by flow cytometry.

The LysoLive Lysosomal Acid Lipase Assay Kit utilizes a sensitive substrate for lysosomal acid lipase (LAL), an enzyme indicated in several diseases such as Lysosomal Acid Lipase Deficiency (LALD). The substrate only becomes fluorescent upon cleavage by the enzyme meaning lysosomes with low acid lipase activity will have reduced staining in a manner proportional to the enzyme activity levels found in the cell.

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What's included?

{ "values": { "1Unit": { "sellingSize": "1 Unit", "publicAssetCode":"ab253380-1Unit", "assetComponentDetails": [ { "size":"5 x 1 Unit", "name":"LysoLive™ LipaGreen", "number":"AB253380-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"MarkerGene™ Flow Holding and Sorting Buffer for non-Adherent Cells", "number":"AB253380-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"MarkerGene™ Flow Holding and Sorting Buffer for Adherent Cells", "number":"AB253380-CMP03", "productcode":"" }, { "size":"1 x 500 µL", "name":"DMSO", "number":"AB253380-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lysosomal acid lipase (LAL) also called lipase A is an essential enzyme with a mass of approximately 43 kDa. It is expressed within the lysosomes of many cell types and catalyzes the hydrolysis of cholesteryl esters and triglycerides into free cholesterol and free fatty acids. This process is important for the cellular lipid metabolism which provides essential signaling molecules and membrane components. The enzyme's activity varies across different tissue types with high expression levels observed in organs like the liver and the adrenal glands.
Biological function summary

Lysosomal acid lipase contributes to the breakdown and regulation of lipid storage within the cell. It is not known to be part of a larger protein complex but its function is critical for maintaining lipid homeostasis in the cell. Through its actions LAL supplies cholesterol and fatty acids essential for various cellular processes including membrane formation and energy production. Additionally it assists in cellular storage management by preventing the excessive accumulation of lipids.

Pathways

The enzymatic actions of lysosomal acid lipase are central to the cholesterol ester and triglyceride metabolic pathways. It plays an important role in the lysosome as part of the lipid degradation process impacting cholesterol transport pathways and maintaining lipid equilibrium. Its activities are closely related to those of other enzymes like hormone-sensitive lipase which also participate in lipid metabolism though within distinct compartments and contexts.

Lysosomal acid lipase is linked to lipid storage disorders such as Wolman disease and Cholesteryl Ester Storage Disease (CESD). These conditions result from LAL deficiency leading to harmful accumulation of cholesteryl esters and triglycerides in tissues. Through disease pathways LAL becomes interconnected with proteins affected by lipid dysregulation such as low-density lipoprotein receptors which play a part in maintaining lipid balance and can contribute to more severe cardiovascular conditions when impaired by LAL deficiency.
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Product protocols

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Target data

Catalyzes the deacylation of cholesteryl ester core lipids of endocytosed low density lipoproteins to generate free fatty acids and cholesterol (PubMed : 15269241, PubMed : 1718995, PubMed : 7204383, PubMed : 8112342, PubMed : 9633819). Hydrolyzes triglycerides (1,2,3-triacylglycerol) and diglycerides (such as 1,2-diacylglycerol and 1,3-diacylglycerol) with preference for the acyl moieties at the sn-1 or sn-3 positions (PubMed : 7204383, PubMed : 8112342).
See full target information LIPA
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4084 PubMed37443159

2023

Liver lipophagy ameliorates nonalcoholic steatohepatitis through extracellular lipid secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshito Minami,Atsushi Hoshino,Yusuke Higuchi,Masahide Hamaguchi,Yusaku Kaneko,Yuhei Kirita,Shunta Taminishi,Toshiyuki Nishiji,Akiyuki Taruno,Michiaki Fukui,Zoltan Arany,Satoaki Matoba

Disease models & mechanisms 16: PubMed37401371

2023

Oxidative stress induces lysosomal membrane permeabilization and ceramide accumulation in retinal pigment epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin R Zhang,Connor S R Jankowski,Rayna Marshall,Rohini Nair,Néstor Más Gómez,Ahab Alnemri,Yingrui Liu,Elizabeth Erler,Julia Ferrante,Ying Song,Brent A Bell,Bailey H Baumann,Jacob Sterling,Brandon Anderson,Sierra Foshe,Jennifer Roof,Hossein Fazelinia,Lynn A Spruce,Jen-Zen Chuang,Ching-Hwa Sung,Anuradha Dhingra,Kathleen Boesze-Battaglia,Venkata R M Chavali,Joshua D Rabinowitz,Claire H Mitchell,Joshua L Dunaief

The Journal of cell biology 222: PubMed37010469

2023

ClC-7 drives intraphagosomal chloride accumulation to support hydrolase activity and phagosome resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ze Wu,Mariia Zeziulia,Whijin Kwon,Thomas J Jentsch,Sergio Grinstein,Spencer A Freeman

GeroScience 44:1407-1440 PubMed35451674

2022

Functional and transcriptional profiling of microglial activation during the chronic phase of TBI identifies an age-related driver of poor outcome in old mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rodney M Ritzel,Yun Li,Zhuofan Lei,Jordan Carter,Junyun He,Harry M C Choi,Niaz Khan,Hui Li,Samantha Allen,Marta M Lipinski,Alan I Faden,Junfang Wu

PloS one 11:e0156312 PubMed27228111

2016

Fluorogenic Substrates for Visualizing Acidic Organelle Enzyme Activities.

Applications

Unspecified application

Species

Unspecified reactive species

Fiona Karen Harlan,Jason Scott Lusk,Breanna Michelle Mohr,Anthony Peter Guzikowski,Robert Hardy Batchelor,Ying Jiang,John Joseph Naleway
View all publications
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