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AB273332

Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric)

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(2 Publications)

Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (ab273332) uses LPC-specific enzymes to generate an intermediate that then reacts with a probe, yielding a signal that can be quantified either colorimetrically or fluorometrically, and is proportional to the amount of LPC present in the sample.
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Functional Studies - Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (AB273332)
  • FuncS

Supplier Data

Functional Studies - Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (AB273332)

Example data.

LPC Standard curve, fluorometric.

Functional Studies - Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (AB273332)
  • FuncS

Supplier Data

Functional Studies - Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (AB273332)

Example data.

LPC Standard curve, colorimetric.

Functional Studies - Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (AB273332)
  • FuncS

Supplier Data

Functional Studies - Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (AB273332)

Example data.

Specificity of the assay : PC = phosphatidylcholine, PA = phosphatidic acid, SM = sphingomyelin, LPC = lysophosphatidylcholine. 1 nmole of each was tested in the fluorometric assay.

Functional Studies - Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (AB273332)
  • FuncS

Supplier Data

Functional Studies - Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (AB273332)

Example data.

Determination of total LPC concentration in pooled human serum. Sample was analyzed according to the kit protocol. Values were determined with fluorometric measurements and are mean ± standard deviation of at least three independent determinations.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Tissue Lysate, Other biological fluids, Cell Lysate

Results type

Quantitative

Assay Platform

Microplate

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Product details

Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (ab273332) utilizes LPC-specific enzymes to generate an intermediate that then reacts with a probe, yielding a signal that can be quantified either colorimetrically or fluorometrically, and is proportional to the amount of LPC present in the sample.

When used as described, the assay is capable of detecting as little as 10 pmole of Lysophosphatidylcholine.

This product is manufactured by BioVision, an Abcam company and was previously called K735 Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric). K735-100 is the same size as the 100 test size of ab273332.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273332-100Test", "assetComponentDetails": [ { "size":"2 x 1 mL", "name":"Lysis Buffer II", "number":"AB273332-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"LPC Standard", "number":"AB273332-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"LPC Developer Mix", "number":"AB273332-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 61", "number":"AB273332-CMP02", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB273332-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"LPC Enzyme Mix", "number":"AB273332-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lysophosphatidylcholine also called LPC serves as an important lipid mediator and is integral in cell membrane composition. LPC has a molecular mass that varies based on the length of its fatty acid chain. It usually serves as a hydrolyzed product of phosphatidylcholine and appears in plasma and tissues. This molecule expresses widely in the human body and its presence is essential for maintaining membrane functionality.
Biological function summary

LPC influences cell proliferation differentiation and apoptosis. It does not operate alone; LPC is often a component of lipoprotein complexes such as low-density lipoproteins. Being part of these complexes LPC can be involved in signaling processes linked to immune and inflammatory responses. Its role in the immune function enhances our understanding of how cellular communication occurs and how cells respond to various stimuli in their environment.

Pathways

Lysophosphatidylcholine participates significantly in the metabolic and inflammatory pathways. It activates the G-protein coupled receptors such as G2A and can mediate effects through the S1P signaling pathway. These pathways link LPC to interactions with proteins like PLA2 which further modulate inflammation and cellular metabolism processes. These biological pathways highlight the importance of LPC in keeping cellular processes balanced and responsive.

Lysophosphatidylcholine is implicated in atherosclerosis and neuroinflammation. LPC levels appear elevated in atherosclerotic lesions possibly due to its role in promoting macrophage recruitment and foam cell formation. Furthermore LPC associates with amyloid-beta peptides in neurodegenerative diseases like Alzheimer’s suggesting a connection with neuroinflammation. Understanding the interaction of LPC with proteins like ApoE gives insight into its pathogenic roles in these diseases.
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Product protocols

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Target data

See full target information Lysophosphatidylcholine
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2406359 PubMed39853712

2025

ACSL1 Aggravates Thromboinflammation by LPC/LPA Metabolic Axis in Hyperlipidemia Associated Myocardial Ischemia-Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Jiang,Xueguang Lin,Bo Chen,Gang Chen,Kristine J S Kwan,Jing Liu,Qi Sun,Jie Wang,Yijie Lu,Jindong Tong,Ying Deng,Bo Yu,Jingdong Tang

Frontiers in pharmacology 15:1406493 PubMed38953111

2024

Ezetimibe, Niemann-Pick C1 like 1 inhibitor, modulates hepatic phospholipid metabolism to alleviate fat accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Hyekyung Yang,Dong Ho Suh,Eun Sung Jung,Yoonjin Lee,Kwang-Hyeon Liu,In-Gu Do,Choong Hwan Lee,Cheol-Young Park
View all publications
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Product promise

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