Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) ab252887 provides a simple way to monitor GAA activity in a wide variety of biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Key facts
- Detection method
- Fluorescent
- Sample types
- Tissue Homogenate, Other biological fluids, Cell Lysate
- Assay type
- Quantitative
- Reactive species
- Mouse, Human
- Sensitivity
- = 0.05 µU
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Essential for the degradation of glycogen in lysosomes (PubMed:14695532, PubMed:18429042, PubMed:1856189, PubMed:7717400). Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans (PubMed:29061980).
Additional Targets
GANAB, GANC
Alternative names
Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, GAA
What's included?
Recommended products
Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) ab252887 provides a simple way to monitor GAA activity in a wide variety of biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Fluorescent
- Sample types
- Tissue Homogenate, Other biological fluids, Cell Lysate
- Assay type
- Quantitative
- Reactive species
- Mouse, Human
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 0.05 µU
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) ab252887 provides a simple way to monitor GAA activity in a wide variety of biological samples. In this kit, GAA cleaves a synthetic specific substrate releasing a fluorophore, which can be easily quantified (Ex/Em= 368/460 nm). The assay is specific and sensitive, it can detect as low as 0.05 μU of GAA activity.
This product is manufactured by BioVision, an Abcam company and was previously called K187 Lysosomal a-Glucosidase (GAA) Activity Assay Kit (Fluorometric). K187-100 is the same size as the 100 test size of ab252887.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha-glucosidase also known as alpha-14-glucosidase is an enzyme with a mass of approximately 105 kDa. It plays an important role in carbohydrate metabolism by catalyzing the hydrolysis of alpha-glycosidic bonds releasing glucose from the non-reducing end of glycogen. This enzyme is mostly present in the brush border of the small intestine where it facilitates the final step of complex carbohydrate digestion. Its activity is essential for the conversion of disaccharides and oligosaccharides into glucose and tools like alpha-glucosidase assays help in quantifying this activity.
- Biological function summary
Alpha-glucosidase activity is important for maintaining normal glucose levels in the blood. It forms part of a larger enzyme complex that operates along the intestinal microvilli. Within the complex it collaborates with other hydrolases to optimize disaccharide breakdown. This role ensures the efficient transformation of ingested carbohydrates into absorbable monosaccharides which is an important cellular energy source.
- Pathways
Alpha-glucosidase participates in the digestive process pathways notably the starch and sucrose metabolism pathway. It acts downstream of amylase enzymes that break down polysaccharides. This enzyme works closely with other glucosidases and maltase-glucoamylase within these pathways to terminate the carbohydrate digestion. Proteins like sucrase-isomaltase also interact in these pathways enhancing carbohydrate assimilation.
- Associated diseases and disorders
Alpha-glucosidase is associated mainly with Pompe disease and diabetes mellitus. Pompe disease results from a defect in the related protein acid alpha-glucosidase (GAA) leading to glycogen accumulation in lysosomes. In diabetes inhibiting intestinal alpha-glucosidase reduces postprandial blood glucose levels making it a target for therapeutic drugs. Understanding these connections aids in developing treatments that modulate enzyme activity to manage carbohydrate metabolism disorders.
Product promise
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3 product images
ELISA - Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) (ab252887)
Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) Example Data.
Measurement of GAA activity in mouse heart extracts (3 μg protein), mouse muscle extracts (2 μg protein) and 3T3 cell lysates (0.5 μg protein).
ELISA - Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) (ab252887)
4-Methylumbelliferone (4-MU) standard curve, results from multiple experiments.
ELISA - Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) (ab252887)
Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) Example Data.
Measurement of GAA activity in human pool serum (5 μl, ultra-concentrate).
Downloads
Product protocols
- Visit the general protocols
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