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AB252887

Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric)

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(1 Publication)

Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) ab252887 provides a simple way to quantify GAA activity. Readout on any fluorometric (Ex/Em 368/460 nm) plate reader.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, GAA, G2AN, KIAA0088, GANAB, Neutral alpha-glucosidase AB, Alpha-glucosidase 2, Glucosidase II subunit alpha, Neutral alpha-glucosidase C, GANC

4 Images
Enzyme activity assay - Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) (AB252887)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) (AB252887)

Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) Example Data.

Measurement of GAA activity in mouse heart extracts (3 μg protein), mouse muscle extracts (2 μg protein) and 3T3 cell lysates (0.5 μg protein).

Enzyme activity assay - Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) (AB252887)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) (AB252887)

Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) Example Data.

Measurement of GAA activity in human pool serum (5 μl, ultra-concentrate).

Enzyme activity assay - Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) (AB252887)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) (AB252887)

4-Methylumbelliferone (4-MU) standard curve, results from multiple experiments.

Other - Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) (AB252887)
  • Other

Supplier Data

Other - Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) (AB252887)

Representative image of Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) ab252887

Components shown from left to right :

- GAA Substrate

- 4-Methyumbelliferone Standard

- GAA Positive Control

- Assay Buffer 25

- Stop Solution V

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Fluorescent

Sample types

Tissue Homogenate, Other biological fluids, Cell Lysate

Reacts with

Mouse, Human

Results type

Quantitative

Sensitivity

= 0.05 µU

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) ab252887 provides a simple way to monitor GAA activity in a wide variety of biological samples. In this kit, GAA cleaves a synthetic specific substrate releasing a fluorophore, which can be easily quantified (Ex/Em= 368/460 nm). The assay is specific and sensitive, it can detect as low as 0.05 μU of GAA activity.

This product is manufactured by BioVision, an Abcam company and was previously called K187 Lysosomal a-Glucosidase (GAA) Activity Assay Kit (Fluorometric). K187-100 is the same size as the 100 test size of ab252887.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab252887-100Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"Assay Buffer 25", "number":"AB252887-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"GAA Positive Control", "number":"AB252887-CMP03", "productcode":"" }, { "size":"1 x 35 µL", "name":"4-Methylumbelliferone Standard", "number":"AB252887-CMP01", "productcode":"" }, { "size":"1 x 280 µL", "name":"GAA Substrate", "number":"AB252887-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Stop Solution V", "number":"AB252887-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-glucosidase also known as alpha-14-glucosidase is an enzyme with a mass of approximately 105 kDa. It plays an important role in carbohydrate metabolism by catalyzing the hydrolysis of alpha-glycosidic bonds releasing glucose from the non-reducing end of glycogen. This enzyme is mostly present in the brush border of the small intestine where it facilitates the final step of complex carbohydrate digestion. Its activity is essential for the conversion of disaccharides and oligosaccharides into glucose and tools like alpha-glucosidase assays help in quantifying this activity.
Biological function summary

Alpha-glucosidase activity is important for maintaining normal glucose levels in the blood. It forms part of a larger enzyme complex that operates along the intestinal microvilli. Within the complex it collaborates with other hydrolases to optimize disaccharide breakdown. This role ensures the efficient transformation of ingested carbohydrates into absorbable monosaccharides which is an important cellular energy source.

Pathways

Alpha-glucosidase participates in the digestive process pathways notably the starch and sucrose metabolism pathway. It acts downstream of amylase enzymes that break down polysaccharides. This enzyme works closely with other glucosidases and maltase-glucoamylase within these pathways to terminate the carbohydrate digestion. Proteins like sucrase-isomaltase also interact in these pathways enhancing carbohydrate assimilation.

Alpha-glucosidase is associated mainly with Pompe disease and diabetes mellitus. Pompe disease results from a defect in the related protein acid alpha-glucosidase (GAA) leading to glycogen accumulation in lysosomes. In diabetes inhibiting intestinal alpha-glucosidase reduces postprandial blood glucose levels making it a target for therapeutic drugs. Understanding these connections aids in developing treatments that modulate enzyme activity to manage carbohydrate metabolism disorders.
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Product protocols

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Target data

Essential for the degradation of glycogen in lysosomes (PubMed : 14695532, PubMed : 18429042, PubMed : 1856189, PubMed : 7717400). Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans (PubMed : 29061980).
See full target information Lysosomal alpha-glucosidase

Additional targets

GANAB,GANC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 74:103234 PubMed38861834

2024

Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyun Guo,Yumeng Li,Shiquan Wang,Yongheng Yang,Tiantian Xu,Jianshuai Zhao,Jin Wang,Wenqiang Zuo,Pengju Wang,Guangchao Zhao,Huaning Wang,Wugang Hou,Hailong Dong,Yanhui Cai
View all publications
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websiteProtocolBooklet
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Product promise

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