JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB112136

Lysosomal Staining Kit - Green Fluorescence - Cytopainter

Be the first to review this product! Submit a review

|

(5 Publications)

Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments.
1 Images
Fluorescence Microscopy - Lysosomal Staining Kit - Green Fluorescence - Cytopainter (AB112136)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Lysosomal Staining Kit - Green Fluorescence - Cytopainter (AB112136)

ab112136 staining U2OS cells by fluorescence microscopy (in a black 96 well plate).

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Assay Platform

Fluorescence microscope

Product details

Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.ab112136 is designed to label lysosomes in live cells with green fluorescence at Ex/Em = 490/525 nm. The proprietary lysotropic dye used in the kit selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and is trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.Review other dyes and kits for , or the

What's included?

{ "values": { "500Test": { "sellingSize": "500 Test", "publicAssetCode":"ab112136-500Test", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Live Cell Staining Buffer", "number":"AB112136-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"LysoGreen Indicator", "number":"AB112136-CMP01", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 20:3653-3670 PubMed40130196

2025

Cutaneous Evaluation of FeO Nanoparticles: An Assessment Based on 2D and 3D Human Epidermis Models Under Standard and UV Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Geanina Watz,Elena-Alina Moacă,Andreea Cioca,Lenuța Maria Șuta,Lavinia Krauss Maldea,Ioana Zinuca Magyari-Pavel,Mirela Nicolov,Ioan-Ovidiu Sîrbu,Felicia Loghin,Cristina A Dehelean

Antioxidants (Basel, Switzerland) 12: PubMed37107193

2023

Membrane Vesicles of Toxigenic Affect the Metabolism of Liver HepG2 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Estefanía Caballano-Infantes,Ailec Ho-Plágaro,Carlos López-Gómez,Flores Martín-Reyes,Francisca Rodríguez-Pacheco,Bernard Taminiau,Georges Daube,Lourdes Garrido-Sánchez,Guillermo Alcaín-Martínez,Raúl J Andrade,Miren García-Cortés,M Isabel Lucena,Eduardo García-Fuentes,Cristina Rodríguez-Díaz

Journal of cellular biochemistry 121:2927-2937 PubMed31692073

2019

Snx10 and PIKfyve are required for lysosome formation in osteoclasts.

Applications

Unspecified application

Species

Unspecified reactive species

Farhath Sultana,Leslie R Morse,Gabriela Picotto,Weimin Liu,Prakash K Jha,Paul R Odgren,Ricardo A Battaglino

International journal of nanomedicine 12:5135-5148 PubMed28790821

2017

Parallel comparative studies on toxicity of quantum dots synthesized and surface engineered with different methods in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Fengjun Liu,Wen Ye,Jun Wang,Fengxiang Song,Yingsheng Cheng,Bingbo Zhang

International journal of molecular sciences 16:21658-80 PubMed26370983

2015

A Novel Nanoprobe for Multimodal Imaging Is Effectively Incorporated into Human Melanoma Metastatic Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Synnøve Nymark Aasen,Aneta Pospisilova,Tilo Wolf Eichler,Jiri Panek,Martin Hruby,Petr Stepanek,Endy Spriet,Daniel Jirak,Kai Ove Skaftnesmo,Frits Thorsen
View all publications
websiteProtocolBooklet
en

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com