Lysosomal Staining Kit - Green Fluorescence (Ex405nm) - Cytopainter

Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

ab112138 is designed to label lysosomes in live cells with green fluorescence of large Stokes Shift at Ex/Em = 405/480nm. ab112138 uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator, a hydrophobic compound, easily permeates intact live cells and gets trapped inside the lysosomes. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components. It is suitable for both suspension and adherent cells.

Review other dyes and kits for lysosomal staining, or the live cell staining fluorescent dyes guide

ab112138 should be stored desiccated.