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Lysosome Isolation Kit (ab234047) provides a procedure for isolating an enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

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Images

Functional Studies - Lysosome Isolation Kit (AB234047), expandable thumbnail
  • Functional Studies - Lysosome Isolation Kit (AB234047), expandable thumbnail

Publications

Key facts

Sample types

Tissue, Suspension cells, Adherent cells

What's included?

50 Test
Components
Density Gradient Media II
1 x 85 mL
Lysosome Enrichment Buffer
1 x 100 mL
Lysosome Isolation Buffer
1 x 25 mL
Protease Inhibitor Cocktail II
1 x 1 mL

Recommended products

Lysosome Isolation Kit (ab234047) provides a procedure for isolating an enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Sample types

Tissue, Suspension cells, Adherent cells

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Lysosome Isolation Kit (ab234047) provides a procedure for isolating an enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation.

This product is manufactured by BioVision, an Abcam company and was previously called K235 Lysosome Purification Kit. K235-50 is the same size as the 50 test size of ab234047.

Lysosomes are membrane-bound cell organelles found in most animal cells (except red blood cells). Lysosomes are spherical vesicles containing hydrolytic enzymes capable of breaking down different kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris. They are known to contain over 50 different enzymes, which are active under acidic conditions. Thus, lysosomes act as the waste disposal system of the cells by digesting unwanted materials in the cytoplasm.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Lysosome Isolation Kit (ab234047), expandable thumbnail

    Functional Studies - Lysosome Isolation Kit (ab234047)

    Location of lysosome band in Discontinuous Density Gradient following ultracentrifugation.

    Tentative Lysosome band location in gradient density.

  • Functional Studies - Lysosome Isolation Kit (ab234047), expandable thumbnail

    Functional Studies - Lysosome Isolation Kit (ab234047)

    Acid Phosphatase (biomarker enzyme for lysosomes) specific activity (mU/mg protein) increases with each purification step.

    Data is normalized by the amount of protein used per well.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com