Lysosome isolation kit (ab234047) provides a procedure for isolating enriched or purified lysosomal fractions from tissues and cultured cells by differential centrifugation, followed by density gradient centrifugation.
- Use lysosomal fractions for downstream applications including activity assays, SDS-PAGE, Western blot
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Proceedings of the National Academy of Sciences of the United States of America 119:e22074021192022Spatiotemporally mapping temperature dynamics of lysosomes and mitochondria using cascade organelle-targeting upconversion nanoparticles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangjun Di,Dejiang Wang,Qian Peter Su,Yongtao Liu,Jiayan Liao,Mahnaz Maddahfar,Jiajia Zhou,Dayong JinPubMed 36322752
- Nature communications 13:48042022Mannose ameliorates experimental colitis by protecting intestinal barrier integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijun Dong,Jingwen Xie,Youyi Wang,Honglian Jiang,Kai Chen,Dantong Li,Jing Wang,Yunzhi Liu,Jia He,Jia Zhou,Liyun Zhang,Xiao Lu,Xiaoming Zou,Xiang-Yang Wang,Qingqing Wang,Zhengliang Chen,Daming ZuoPubMed 35974017
- Journal of virology 96:e00767222022Endocytosed HIV-1 Envelope Glycoprotein Traffics to Rab14 Late Endosomes and Lysosomes to Regulate Surface Levels in T-Cell Lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuxley K Hoffman,Rebekah S Aguilar,Austin R Clark,Nicholas S Groves,Nairi Pezeshkian,Merissa M Bruns,Schuyler B van EngelenburgPubMed 35770989
- International journal of biological sciences 18:3859-38732022Inhibitory role of TRIP-Br1 oncoprotein in anticancer drug-mediated programmed cell death via mitophagy activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamil Jung,Davaajargal Myagmarjav,Taeyeon Jo,Soonduk Lee,Songyi Han,Nguyen Thi Ngoc Quynh,Nguyen Hai Anh,Son Hai Vu,Yeongseon Choi,Myeong-Sok LeePubMed 35813469
- eLife 11:2022Protein-lipid interaction at low pH induces oligomerization of the MakA cytotoxin from .Applications:Unspecified applicationReactive species:Unspecified reactive speciesAftab Nadeem,Alexandra Berg,Hudson Pace,Athar Alam,Eric Toh,Jörgen Ådén,Nikola Zlatkov,Si Lhyam Myint,Karina Persson,Gerhard Gröbner,Anders Sjöstedt,Marta Bally,Jonas Barandun,Bernt Eric Uhlin,Sun Nyunt WaiPubMed 35131030
- Autophagy 18:2036-20492021DCN released from ferroptotic cells ignites AGER-dependent immune responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiao Liu,Shan Zhu,Ling Zeng,Jingbo Li,Daniel J Klionsky,Guido Kroemer,Jianxin Jiang,Daolin Tang,Rui KangPubMed 34964698
- Nature 596:570-5752021Tonic prime-boost of STING signalling mediates Niemann-Pick disease type C.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing-Ting Chu,Xintao Tu,Kun Yang,Jianjun Wu,Joyce J Repa,Nan YanPubMed 34290407
- STAR protocols 2:1003492021Synthesis of an Hsp70 inhibitor and its assessment of lysosomal membrane permeabilization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSang-Hyun Park,Sookil Park,Injae ShinPubMed 33665629
- Science advances 7:2021Neurodevelopmental defects and neurodegenerative phenotypes in human brain organoids carrying Parkinson's disease-linked mutations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNoviana Wulansari,Wahyu Handoko Wibowo Darsono,Hye-Ji Woo,Mi-Yoon Chang,Jinil Kim,Eun-Jin Bae,Woong Sun,Ju-Hyun Lee,Il-Joo Cho,Hyogeun Shin,Seung-Jae Lee,Sang-Hun LeePubMed 33597231
Key facts
- Sample types
- Tissue, Suspension cells, Adherent cells
What's included?
Recommended products
Lysosome isolation kit (ab234047) provides a procedure for isolating enriched or purified lysosomal fractions from tissues and cultured cells by differential centrifugation, followed by density gradient centrifugation.
- Use lysosomal fractions for downstream applications including activity assays, SDS-PAGE, Western blot
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Sample types
- Tissue, Suspension cells, Adherent cells
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Lysosome Isolation Kit (ab234047) provides a procedure for isolating an enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation.
Lysosome Isolation protocol summary
- Isolate and homogenize cells or tissue
- Centrifuge homogenate and keep supernatent
- Carefully add supernatent to discontinuous density gradient
- Remove band containing enriched lysosome fraction
- Use for downstream applications e.g. activity assays, SDS-PAGE, Western blot
This product is manufactured by BioVision, an Abcam company and was previously called K235 Lysosome Purification Kit. K235-50 is the same size as the 50 test size of ab234047.
Lysosomes are membrane-bound cell organelles found in most animal cells (except red blood cells). Lysosomes are spherical vesicles containing hydrolytic enzymes capable of breaking down different kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris. They are known to contain over 50 different enzymes, which are active under acidic conditions. Thus, lysosomes act as the waste disposal system of the cells by digesting unwanted materials in the cytoplasm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Lysosome Isolation Kit (ab234047)
Acid Phosphatase (biomarker enzyme for lysosomes) specific activity (mU/mg protein) increases with each purification step.
Data is normalized by the amount of protein used per well.
Functional Studies - Lysosome Isolation Kit (ab234047)
Location of lysosome band in Discontinuous Density Gradient following ultracentrifugation.
Tentative Lysosome band location in gradient density.
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