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AB211113

Lysozyme Activity Assay Kit (Fluorometric)

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(4 Publications)

Lysozyme Activity Assay Kit (ab211113) provides a convenient method for quantifying Lysozyme activity in mammalian cell or tissue lysates, biological fluids such as tears or saliva.
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Functional Studies - Lysozyme Activity Assay Kit (Fluorometric) (AB211113)
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Supplier Data

Functional Studies - Lysozyme Activity Assay Kit (Fluorometric) (AB211113)

Measurement of Lysozyme activity in human fluids. Assays were performed following kit protocols.

Functional Studies - Lysozyme Activity Assay Kit (Fluorometric) (AB211113)
  • FuncS

Supplier Data

Functional Studies - Lysozyme Activity Assay Kit (Fluorometric) (AB211113)

Measurement of Lysozyme activity in human cultured cell lysates. Assays were performed following kit protocols.

Functional Studies - Lysozyme Activity Assay Kit (Fluorometric) (AB211113)
  • FuncS

Supplier Data

Functional Studies - Lysozyme Activity Assay Kit (Fluorometric) (AB211113)

Typical 4-Methylumbelliferon (4-MU) Standard Curve.

Other - Lysozyme Activity Assay Kit (Fluorometric) (AB211113)
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Supplier Data

Other - Lysozyme Activity Assay Kit (Fluorometric) (AB211113)

Representative image of Lysozyme Activity Assay Kit (Fluorometric) ab211113

Components shown from left to right :

- Lyzozyme Substrate

- 4-Methyumbelliferone Standard

- Lysozyme Positive Control

- Assay Buffer 21

- Lysozyme Stop Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Fluorescent

Sample types

Purified protein, Tissue Lysate, Saliva, Cell culture extracts, Tissue Homogenate, Serum, Cell Lysate

Assay type

Enzyme activity

Results type

Quantitative

Sensitivity

= 0.002 U/mL

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Lysozyme Activity Assay Kit (ab211113) provides a convenient method for quantifying Lysozyme activity in mammalian cell or tissue lysates, biological fluids such as tears or saliva. This product can also be used to measure lysozyme activity in yeast and bacteria. The assay is based on the ability of lysozyme to cleave a synthetic substrate and release a free fluorophore which can be easily quantified at Ex/Em= 360/445 nm in a fluorescent microplate reader.

This kit provides a simple, ultra-sensitive assay that can detect as low as 2 uU/mL of Lysozyme activity.

Other Notes
This product was previously called K236 Biovision Lysozyme Activity Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.

Lysozyme (EC 3.2.1.17), also known as muramidase or N-acetylmuramide glycanhydrolase, is a hydrolase acting on glycosidic bonds. It hydrolyzes the Beta-(1-4)-glucosidic linkage between N-acetyl-muraminic acid and N-acetyl-D-glucosamine residues present in the mucopolysaccharide cell wall. Lysozyme is ubiquitously found in a wide range of biological fluids such as tears, saliva and tissues. It exhibits antibacterial, antitumor and immune modulatory activities. Elevated concentrations of lysozyme in urine and serum have been reported in patients suffering leukemia, tuberculosis, megaloblastic anemias, acute bacterial infections, ulcerative colitis, severe renal insufficiency, pyelonephritis and nephritis.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab211113-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Lysozyme Positive Control", "number":"AB211113-CMP02", "productcode":"" }, { "size":"1 x 35 µL", "name":"4-Methylumbelliferone Standard", "number":"AB211113-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"Lysozyme Stop Buffer", "number":"AB211113-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 21", "number":"AB211113-CMP05", "productcode":"" }, { "size":"1 x 65 µL", "name":"Lyzozyme Substrate", "number":"AB211113-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lysozyme also known as muramidase is a protein with enzymatic activity that breaks down bacterial cell walls by hydrolyzing the glycosidic bonds of peptidoglycan. The molecular weight of lysozyme is approximately 14 kDa. This protein is expressed in various tissues and body fluids including egg whites human saliva tears and milk. Lysozyme acts as an antimicrobial agent and plays a significant role in the innate immune system.
Biological function summary

Lysozyme contributes to host defense by targeting bacteria. It is not part of a complex but functions as a single enzyme. By compromising the integrity of bacterial cell walls it clears pathogens swiftly from the body. Lysozyme activity assays measure its ability to hydrolyze bacterial peptidoglycan reflecting its role in immune defense. Many cells such as neutrophils and macrophages express lysozyme during the immune response aiding in pathogen clearance.

Pathways

Lysozyme is chiefly involved in the antibacterial humoral response pathway. Its action aligns with other antimicrobial proteins such as lactoferrin. Both lysozyme and lactoferrin are integral to the body's first line of defense against bacterial invasion. In addition lysozyme is relevant in the pathway managing mucosal immunity working in conjunction with proteins that enhance pathogen clearance like defensins.

Lysozyme has links to conditions such as sarcoidosis and hereditary renal amyloidosis. In sarcoidosis altered expression patterns of lysozyme may serve as a marker for disease activity. In hereditary renal amyloidosis mutations in the lysozyme gene result in amyloid fibril formation leading to renal dysfunction. Understanding these connections helps in the diagnosis and treatment of related conditions.
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Product protocols

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Target data

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Rapid communications in mass spectrometry : RCM 39:e10048 PubMed40255129

2025

Buffer 4-Ethylmorpholinium/Acetate: Exploring a New Alternative Buffer for Native Mass Spectrometry.

Applications

Unspecified application

Species

Unspecified reactive species

Darya Hadavi,Che Yee Ng,Yuandi Zhao,Anjusha Mathew,Ian G M Anthony,Berta Cillero-Pastor,Eva Cuypers,Tiffany Porta Siegel,Maarten Honing

Saudi journal of biological sciences 30:103806 PubMed37766887

2023

Interleukin-35 and Thymoquinone nanoparticle-based intervention for liver protection against paracetamol-induced liver injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Maisa Siddiq Abduh,Sultan Ayesh Mohammed Saghir,Naif Ahmed Al-Gabri,Ahmad Faheem Ahmeda,Mouaadh Abdelkarim,Saleh Mohammad Aldaqal,Mohammed Abdullah Alshawsh

Nature plants 9:420-432 PubMed36805038

2023

Tight genetic linkage of genes causing hybrid necrosis and pollinator isolation between young species.

Applications

Unspecified application

Species

Unspecified reactive species

Chaobin Li,Marta Binaghi,Vivien Pichon,Gina Cannarozzi,Loreta Brandão de Freitas,Mathieu Hanemian,Cris Kuhlemeier

Immunity 53:398-416.e8 PubMed32814028

2020

Paneth Cell-Derived Lysozyme Defines the Composition of Mucolytic Microbiota and the Inflammatory Tone of the Intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyan Yu,Iyshwarya Balasubramanian,Daniel Laubitz,Kevin Tong,Sheila Bandyopadhyay,Xiang Lin,Juan Flores,Rajbir Singh,Yue Liu,Carlos Macazana,Yanlin Zhao,Fabienne Béguet-Crespel,Karuna Patil,Monica T Midura-Kiela,Daniel Wang,George S Yap,Ronaldo P Ferraris,Zhi Wei,Edward M Bonder,Max M Häggblom,Lanjing Zhang,Veronique Douard,Michael P Verzi,Ken Cadwell,Pawel R Kiela,Nan Gao
View all publications
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