Lysyl Oxidase Activity Fluorometric Assay Kit (ab284573) (K928) allows for quantitative evaluation of lysyl oxidase activity of purified enzyme and its inhibitors as well as secreted endogenous enzyme in cell media.
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Key facts
- Detection method
Fluorescent
- Sample types
Purified protein, Cell culture media
- Assay type
Enzyme activity (quantitative)
- Reactive species
Mammals
- Sensitivity
>= 40 ng
Target data
Function
Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin (PubMed:26838787). Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture (By similarity).
Alternative names
Protein-lysine 6-oxidase, Lysyl oxidase, LOX
What's included?
Recommended products
Lysyl Oxidase Activity Fluorometric Assay Kit (ab284573) (K928) allows for quantitative evaluation of lysyl oxidase activity of purified enzyme and its inhibitors as well as secreted endogenous enzyme in cell media.
Alternative names
Protein-lysine 6-oxidase, Lysyl oxidase, LOX
Key facts
- Detection method
Fluorescent
- Sample types
Purified protein, Cell culture media
- Assay type
Enzyme activity (quantitative)
- Reactive species
Mammals
- Assay Platform
Microplate
- Sensitivity
>= 40 ng
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Lysyl Oxidase Activity Fluorometric Assay Kit (ab284573) (K928) allows for quantitative evaluation of lysyl oxidase activity of purified enzyme and its inhibitors as well as secreted endogenous enzyme in cell media. Enzyme activity is detected upon oxidation of substrate, which results in release of reaction intermediates. Reaction intermediates are subsequently detected by LOX Probe, resulting in fluorescent signal that can be measured at Ex/Em= 535/587 nm.
Each kit includes a positive control as well as a specific lysyl oxidase inhibitor that allows the user to correct each sample for any non-specific signal. Lysyl Oxidase Activity Fluorometric Assay Kit can detect as little as 40 ng of recombinant enzyme in vitro.
This product is manufactured by BioVision, an Abcam company and was previously called K928 Lysyl Oxidase Activity Assay Kit (Fluorometric). K928-100 is the same size as the 100 test size of ab284573.
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2 product images
Functional Studies - Lysyl Oxidase Activity Assay Kit (Fluorometric) (ab284573)
Enzyme activity at varying concentrations of recombinant Lysyl Oxidase.
Functional Studies - Lysyl Oxidase Activity Assay Kit (Fluorometric) (ab284573)
Activity curve of abactivity curve.
Representative activity curve for cell media sample and cell media spike standard at 37°C, where cell media is test sample, cell media and inhibitor is negative control, and 150 pmol spike standard is sample and spike standard.
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