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AB284573

Lysyl Oxidase Activity Assay Kit (Fluorometric)

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Lysyl Oxidase Activity Fluorometric Assay Kit (ab284573) (K928) allows for quantitative evaluation of lysyl oxidase activity of purified enzyme and its inhibitors as well as secreted endogenous enzyme in cell media.

View Alternative Names

Protein-lysine 6-oxidase, Lysyl oxidase, LOX

2 Images
Functional Studies - Lysyl Oxidase Activity Assay Kit (Fluorometric) (AB284573)
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Supplier Data

Functional Studies - Lysyl Oxidase Activity Assay Kit (Fluorometric) (AB284573)

Activity curve of abactivity curve.

Representative activity curve for cell media sample and cell media spike standard at 37°C, where cell media is test sample, cell media and inhibitor is negative control, and 150 pmol spike standard is sample and spike standard.

Functional Studies - Lysyl Oxidase Activity Assay Kit (Fluorometric) (AB284573)
  • FuncS

Supplier Data

Functional Studies - Lysyl Oxidase Activity Assay Kit (Fluorometric) (AB284573)

Enzyme activity at varying concentrations of recombinant Lysyl Oxidase.

Key facts

Detection method

Fluorescent

Sample types

Purified protein, Cell culture media

Assay type

Enzyme activity

Results type

Quantitative

Sensitivity

>= 40 ng

Assay Platform

Microplate

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Primary Antibodies

AB260043

Anti-Collagen I antibody [EPR22894-89]

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Western blot - Anti-Collagen I antibody [EPR22894-89] (AB260043)

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Primary Antibodies

AB186733

Anti-Argonaute-2 antibody [EPR10411]

KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Argonaute-2 antibody [EPR10411] (AB186733)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Lysyl Oxidase Activity Fluorometric Assay Kit (ab284573) (K928) allows for quantitative evaluation of lysyl oxidase activity of purified enzyme and its inhibitors as well as secreted endogenous enzyme in cell media. Enzyme activity is detected upon oxidation of substrate, which results in release of reaction intermediates. Reaction intermediates are subsequently detected by LOX Probe, resulting in fluorescent signal that can be measured at Ex/Em= 535/587 nm.

Each kit includes a positive control as well as a specific lysyl oxidase inhibitor that allows the user to correct each sample for any non-specific signal. Lysyl Oxidase Activity Fluorometric Assay Kit can detect as little as 40 ng of recombinant enzyme in vitro.

This product is manufactured by BioVision, an Abcam company and was previously called K928 Lysyl Oxidase Activity Assay Kit (Fluorometric). K928-100 is the same size as the 100 test size of ab284573.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab284573-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Lysyl Oxidase Positive Control", "number":"AB284573-CMP01", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB284573-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"H2O2 Standard", "number":"AB284573-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution V", "number":"AB284573-CMP06", "productcode":"" }, { "size":"1 x 45 mL", "name":"Lysyl Oxidase Assay Buffer", "number":"AB284573-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lysyl Oxidase Inhibitor", "number":"AB284573-CMP07", "productcode":"" }, { "size":"1 x 100 µL", "name":"Lysyl Oxidase Substrate", "number":"AB284573-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin (PubMed : 26838787). Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture (By similarity).
See full target information Protein-lysine 6-oxidase
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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