AB326115
m6A Methylase Activity/Inhibition Assay Kit (Colorimetric)
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The m6A Methylase Activity/Inhibition Assay Kit (Colorimetric) is a complete set of optimized buffers and reagents designed for measuring the activity/inhibition of total m6A methylases (methyltransferases) using nuclear extracts or purified m6A methylases like METTL3/METTL14 from a broad range of species such as mammalian, plant, fungal, and bacterial, in a variety of forms including, but not limited to, cultured cells and, fresh and frozen tissues.
2 Images
- Enzyme activity assay
Supplier Data
Enzyme activity assay - M6A Methylase Activity/Inhibition Assay Kit (Colorimetric) (AB326115)
m6A methylase activity achieved by using recombinant METTL3/14 with the m6A Methylase Activity/Inhibition Assay Kit (Colorimetric) (ab326115). Recombinant human METTL3/14 enzyme was added at different concentrations.
- Enzyme activity assay
Supplier Data
Enzyme activity assay - M6A Methylase Activity/Inhibition Assay Kit (Colorimetric) (AB326115)
Illustrated standard curve generated with the m6A methylase assay standard.
Reactivity data
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Product details
The m6A Methylase Activity/Inhibition Assay Kit (Colorimetric) is a complete set of optimized buffers and reagents designed for measuring the activity/inhibition of total m6A methylases (methyltransferases) using nuclear extracts or purified m6A methylases like METTL3/METTL14 from a broad range of species such as mammalian, plant, fungal, and bacterial, in a variety of forms including, but not limited to, cultured cells and, fresh and frozen tissues. This kit has the following advantages and features:
- Fast: Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours.
- Robust: Innovative kit composition enables background signals to be extremely low and allows the assay to be simple, accurate, reliable, and consistent.
- Convenient: Both cell/tissue nuclear extracts and purified proteins can be used, which allows detection of inhibitory effects of m6A methylase inhibitor in vivo and in vitro.
- Sensitive and Specific: Novel assay principle allows to detect the m6A methylase-converted end product, which makes the assay much more specific than that for by-product measurement. The assay also allows high sensitivity to be achieved. The activity can be detected from as low as 2 µg of nuclear extracts or 50 ng of purified enzymes.
- Quantitative: The assay standard is included, which allows the specific activity of m6A methylase to be quantified.
- Flexible: Strip-well microplate format makes the assay flexible for manual or high throughput analysis.
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
Product protocols
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