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AB185912

m6A RNA Methylation Assay Kit (Colorimetric)

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(197 Publications)

m6A RNA Methylation Assay Kit (Colorimetric) ab185912 is an m6a ELISA kit with a complete set of optimized buffers and reagents to quantify methylated N6-methyladenosine (m6A) in RNA. Readout on any colorimetric (450 nm) plate reader.

- Sensitivity as low as 10 pg of m6A
- suitable for quantifying m6A from any species
- Cited in >130 publications

View Alternative Names

N6-methyladenosine, m6A

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ELISA - m6A RNA Methylation Assay Kit (Colorimetric) (AB185912)
  • ELISA

Supplier Data

ELISA - m6A RNA Methylation Assay Kit (Colorimetric) (AB185912)

Quantification of m6A RNA methylation in different samples. 200 ng of RNA isolated from different tissues or cells were added into the assay wells and the m6A contained in RNA was measured using the m6A RNA Methylation Quantification Kit (Colorimetric).

Functional Studies - m6A RNA Methylation Assay Kit (Colorimetric) (AB185912)
  • FuncS

Supplier Data

Functional Studies - m6A RNA Methylation Assay Kit (Colorimetric) (AB185912)

m6A Standard curve.

m6A standard control was added into the assay wells at different concentrations and then measured with the m6A RNA Methylation Quantification Kit (Colorimetric).

Key facts

Detection method

Colorimetric

Assay type

Quantitative

Sensitivity

= 0.01 ng

Range

10 - 500 ng/mL

Assay time

3h 45m

Assay Platform

Microplate reader

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Product details

m6A ELISA Kit / m6A RNA Methylation Assay Kit ab185912 is suitable for the direct detection of m6A RNA methylation status using total RNA isolated from any species such as mammals, plants, fungi, bacteria, and viruses.

Be aware that m6A is present in mRNA and ribosomal RNA, and this kit detects m6A in total RNA, ie. both mRNA and ribosomal RNA and any other RNA containing m6A.

The amount of RNA for each assay can be 100 ng - 300 ng. For optimal quantification, the input RNA amount should be 200 ng, as the abundance of m6A is generally less than 0.1% of total RNA.

m6A ELISA / m6A RNA methylation assay protocol summary

- Total RNA is bound to strip wells using a RNA high binding solution
- N6-methyladenosine (m6A) is detected using a specific capture N6-methyladenosine antibody and detection antibody
- The detected signal is enhanced and then quantified colorimetrically by reading the absorbance in a microplate spectrophotometer at a wavelength of 450 nm. The amount of m6A is proportional to the OD intensity measured.
- A standard curve can be performed (range: 0.02 to 1 ng of m6A) or a single quantity of m6A can be used as a positive control.

How other researchers are using

m6A RNA Methylation Quantification Kit (Colorimetric) has been used in a variety of sample type including:
- Tumor and control peritoneal tissues 1
- Human osteosarcoma cancer cell lines 143B and U2OS 2
- Human lung epithelial cell line BEAS-2B, Human bronchial epithelial cell line 16HBE 3

References:
1 - Reamon-Buettner S et al. 2024; 2 - Mei Z et al. 2024; 3 - Ma L et al. 2024.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N6-methyladenosine (m6A) is a chemical modification present in RNA molecules specifically marking the adenosine base with a methyl group at the nitrogen-6 position. This modification weighing approximately 14 Da occurs in diverse eukaryotic species and influences RNA metabolism. m6A methylation can be detected using techniques like m6A dots blot or m6A ELISA. The modification is abundant in tissues like the brain and testis reflecting its critical role in varying cell types. Also m6A is known as a dynamic and reversible mark with its levels continuously adjusted by enzymes called writers (methyltransferases) erasers (demethylases) and readers (RNA-binding proteins).
Biological function summary

M6A methylation affects mRNA processing stability translation and decay. It integrates into large multi-protein complexes where it influences gene expression outcomes by affecting the RNA's interaction with the cellular machinery. This methylation modification acts as a regulatory signal that influences essential processes such as cell differentiation and circadian rhythms. Elucidating the biological functions of m6A involves studying how it affects RNA fate and its downstream gene regulatory networks.

Pathways

M6A modification is central to the mRNA metabolic pathway and the PI3K-Akt signaling pathway. It interacts with various proteins such as METTL3 an m6A methyltransferase which is vital for mediating m6A modification. It also interacts with YTH domain-containing proteins that recognize m6A marks influencing transcript dynamics and gene expression. The interplay of m6A with proteins in these pathways underlines its role in fine-tuning cellular processes and responses.

M6A modification has a significant impact on cancer and neurological disorders. In cancers alterations in m6A methylation patterns can promote oncogenic transformation and metastasis. m6A-related proteins such as FTO an m6A demethylase have shown connections to these pathways affecting cancer progression. In neurological disorders m6A impacts aspects of neural development and function and abnormalities in its regulation may contribute to diseases like Alzheimer's. Understanding the roles of m6A in diverse diseases can pave the way for novel therapeutic approaches.
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Product protocols

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Target data

See full target information N6-methyladenosine (m6A)
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Publications (197)

Recent publications for all applications. Explore the full list and refine your search

Genes & diseases 12:101519 PubMed40612667

2025

Restriction of YWHAB-mediated YAP cytoplasmic retention is a novel mechanism underlying stemness maintenance and chemoresistance in ovarian cancer peritoneal metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Lei Shi,Zijun Meng,Manlin Zhang,Zhiqi Zhang,Yunzhe Li,Kaiwen Du,Muyao Yang,Lin Qiu,Jing Feng,Yuchen He,Jiayun Liu,Hua Zhang,Hongbin Zhang,Tingyuan Lang,Zhuo Yang

Cell death discovery 11:294 PubMed40592832

2025

N6-methyladenosine modification of MEF2A weakens cetuximab sensitivity in colorectal cancer via PD-L1/SOX12 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Cao Gao,Jiajia He,Jiemin Zhao,Xuefeng Ni,Yanjie Xu

International journal of molecular sciences 26: PubMed40508042

2025

Betaine Alleviates Bisphosphonate-Related Osteonecrosis of the Jaw by Rescuing BMSCs Function in an m6A-METTL3-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhou Jin,Jiaxin Song,Zhanqiu Diao,Xiao Han,Zhipeng Fan

CytoJournal 22:38 PubMed40469707

2025

Methyltransferase 3-mediated m6A modification of Switch/sucrose non-fermenting-related matrix-associated actin-dependent regulator of chromatin subfamily a member 5 promotes mycobacterium tuberculosis-infected macrophage M1 polarization and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Chen,Hai Huang

Nature communications 16:4457 PubMed40368943

2025

Subcellular level spatial transcriptomics with PHOTON.

Applications

Unspecified application

Species

Unspecified reactive species

Shreya Rajachandran,Qianlan Xu,Qiqi Cao,Xin Zhang,Fei Chen,Sarah M Mangiameli,Haiqi Chen

Renal failure 47:2497492 PubMed40325507

2025

METTL3 promotes podocyte pyroptosis in diabetic nephropathy through N-methyladenosine modification of TRIM29 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Xu,Xiaolin Huang,Ce Zhang,Xia Mi,Chi Zhang,Fei Hua,Liexiang Zhang

Nature communications 16:4063 PubMed40307231

2025

The motor neuron m6A repertoire governs neuronal homeostasis and FTO inhibition mitigates ALS symptom manifestation.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ping Yen,Ting-Hsiang Lung,Ee Shan Liau,Chuan-Che Wu,Guan-Lin Huang,Fang-Yu Hsu,Mien Chang,Zheng-Dao Yang,Chia-Yi Huang,Zhong Zheng,Wei Zhao,Jui-Hung Hung,Chuan He,Qing Nie,Jun-An Chen

Cellular and molecular life sciences : CMLS 82:157 PubMed40220148

2025

m6A reader IGF2BP1 facilitates macrophage glycolytic metabolism and fibrotic phenotype by stabilizing THBS1 mRNA to promote pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Hu,Lulu Yang,Liting Huang,Chao Zeng,Siying Ren

PLoS pathogens 21:e1013064 PubMed40198675

2025

N6-methyladenosine modification of the subgroup J avian leukosis viral RNAs attenuates host innate immunity via MDA5 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Yu,Li Zhang,Ying Wang,Suyan Wang,Yongzhen Liu,Peng Liu,Yuntong Chen,Ru Guo,Lingzhai Meng,Tao Zhang,Wenrui Fan,Xiaole Qi,Yulu Duan,Yanping Zhang,Hongyu Cui,Yulong Gao

Molecular biomedicine 6:17 PubMed40097750

2025

M6A-modified BFSP1 induces aerobic glycolysis to promote liver cancer growth and metastasis through upregulating tropomodulin 4.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Li,Shunle Li,Lin Shen,Junhui Li,Di Zhang,Jinmin Yu,Lanxuan Huang,Na Liu,Hongwei Lu,Meng Xu
View all publications
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